Українська English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2017; 63(6): 66-71


L.M. Plotnikova, V.Y. Berezovskyi, M.I. Levashov, O.G. Chaka, R.V. Yanko

    O.O. Bogomoletz Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine


We investigated the effect of intermittent normobaric hypoxia (INH) of sanogenic level and melatonin (5 mg/kg) on locomotor and emotional activity of young Wistar rats in the test «open field». The animals were divided into two groups: with low and high level of energy metabolism (LEM). Total locomotor activity in rats with high LEM control group was significantly lower by 17 % relatively low LEM rats. It was shown that joint ingestion of INH and melatonin increased horizontal motor activity by 22-38 %, decreased emotionality of rats in 2,5-2,7 times irrespective of their level of energy metabolism. In animals with low LEM, hypoxia, both separately and together with melatonin, increased the frequency (by 4,0 and 8,7 times, respectively) and the duration of the grooming (in 1,7 and 4,9 times, respectively) compared with the intact group. After 28 sessions of INH in them, the weight of the body significantly decreased by 10 % compared to the control. The combined action of INH and melatonin activated the orienting and research behavior of rats, reduced the level of anxiety, which may indicate an anti-stress effect of such influence. Key words:

Keywords: Intermittent normobaric hypoxia; melatonin; level of energy metabolism; behavioral response


  1. Hill R, McKernan L, Wang L, Coronado R. Changes in psychosocial well-being after mindfulness-based stress reduction: a prospective cohort study. J Man Manip Ther. 2017; 25(3):128-36. CrossRef PubMed
  3. Kaidashev I. Changing lifestyles, the violation of energy metabolism and systemic inflammation as factors of civilization diseases. Ukr Med Magazine. 2013;5(97):103-8. [Russian].
  5. Gostyukhina A, Zamoshchina T, Svetlik M, Zhukova O, Zaitsev K, Abdulkina N. Behavioral activity of rats in the «open field» after the light and dark deprivation and physical exhaustion. Bulletin of Siberian Medicine. 2016;15(3):16-23. [Russian]. CrossRef  
  6. Shi L, Li N, Bo L, Xu Z. Melatonin and hypothalamic-pituitary-gonadal axis. Cur Med Chem. 2013; 20(15):2017-31. CrossRef  
  7. Arushanyan E, Naumov S. Anti-inflammatory potential of melatonin. Clinical medicine. 2013; 91(7):18-22.
  9. Rodriguez C, Mayo J, Sainz R, Antolnn I, Herrera F, Martnn V, Reiter R. Regulation of antioxidant enzymes: a significant role for melatonin. Jour Pin Res. 2004;36(1):1-9. CrossRef  
  10. Tsebrzhynskyy O, Frenkel Y. Features of testing «open field» rats that had hyper- and hipomelatoninemiyi. The World of Medicine and Biology. 2006; 3:47-52. [Ukrainian].
  12. Frankel Y. Assessment of behavioral laboratory rats in the test «open field» when playing the experimentally hyper- and hipomelatoninemii. Modern health problems, radiation and environmental medicine: Coll Scientific articles. 2014;4: 306-10. [Russian].
  14. Berezovsky V. Natural and instrumental oroterapiya. Donetsk: Publisher Zaslavsky AJ. 2012: 304 p. [Russian].
  16. Serebrovskaya T, Shatilo V. Experience of using interval hypoxia in prevention and treatment of cardiovascular diseases: Review. Blood Circulation and Hemostasis. 2014;1-2:16-33. [Russian].
  18. Balabekova M, Ryspekova N, Zhukesheva M, Askarova A, Nurmuhambetov A. Study of behavioral reactions of old rats treated with hypoxia. Medical Sciences. 2014; 7:662-6. [Russian].
  20. Ol'nyanskaya R, Isaakyan L. Research methods gas exchange in humans and animals. Moscow: Medgiz. 1959:180 p. [Russian].
  22. Buresh J, Bureshova O, Houston P. The methodology and basic experiments for the study of brain and behavior. Moscow: High school. 1991: 299 p. [Russian]. PubMedCentral
  24. Kulikov V. Individual features of spontaneous locomotor activity and adaptive behavior in rats. Zh Higher Nerve Activity. 1990; 40(1):85-92. [Russian].
  26. Vlizlo V, Fedoruk R, Ratych I. Laboratory methods of research in biology, veterinary medicine. Lviv. 2012: 762 p. [Ukrainian].
  28. Shevereva V. Influence of chronical emotional stress on the behaviour in open field of rets with different level of motional activity. Fiziol. Zh. 2011;57(1):94-105. [Ukrainian].
  30. Gridin L. Modern understanding of the physiological and therapeutic and prophylactic effects of actions hypoxia and hypercapnia. Journal of Medicine. 2016;3:45-68. [Russian].
  32. Chen C, Tsa Y, Kao C, Lee S, Wu M, Mallikarjuna K, Liao Y, Ivy J, Kuo C. Effect of mild intermittent hypoxia on glucose tolerance, muscle morphology and AMPKPGC-1alpha signaling. Chin J Physiol. 2010; 53(1):62-71. CrossRef PubMed
  34. Sun H, Gusdon A, Qu S. Effects of melatonin on cardiovascular diseases: progress in the past year. Curr Opin Lipidol. 2016; 27(4):408-13. CrossRef PubMed PubMedCentral

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2024.