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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2017; 63(6): 3-8


ENDOTHELIAL MONOCYTE-ACTIVATING POLYPEPTIDE-II IMPROVES DIASTOLIC HEART FUNCTION AND VASCULAR RELAXATION IN STREPTOZOTOCIN-INDUCED EXPERIMENTAL DIABETES

N.A. Dorofeyeva1, G.G. Vorobyov1, I.Y. Cherepaha1, A.I. Kornelyuk2, V.F. Sagach1

  1. Bogomoletz Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv
  2. The Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kyiv
DOI: https://doi.org/10.15407/fz63.06.003

Abstract

To investigate the effects of EMAP II on cardiac function and endothelium-dependent relaxation of smooth muscles of the aorta in diabetes mellitus, studies were conducted on 6 monthsold male streptozotocin-induced diabetic rats and control rats. In experiments in vivo, we have shown that EMAP II improves diastolic heart function in streptozotocin-induced diabetic rats. The End-diastolic pressure in streptozotocin-induced diabetic rats was decreased by 25,4% (P<0,05), the end-diastolic myocardial stiffness decreased by 25,5% (P<0,05). After EMAP II the end-systolic pressure decreased by 13,8% and the arterial stiffness decreased by 26,3% (P<0,05). In vitro experiments on II promotes the improvement and restoration of endotheliumdependent relaxation in streptozotocin-induced diabetic rats. Inhibition of NOS activity with L-NAME abolished EMAP II-mediated vasodilatation in the endothelium-intact aorta that indicates the NOS-dependent mechanism of action of EMAP II. Thus, EMAP II had a positive effect on the heart and vessels of rats with streptozotocin-induced diabetes, improving diastolic heart function and vasorelaxation in diabetes.

Keywords: Endothelial monocyte-activating polypeptide II (EMAP II); diabetes; endothelium-dependent relaxation of the aorta; heart

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