ENDOTHELIAL MONOCYTE-ACTIVATING POLYPEPTIDE-II IMPROVES DIASTOLIC HEART FUNCTION AND VASCULAR RELAXATION IN STREPTOZOTOCIN-INDUCED EXPERIMENTAL DIABETES
N.A. Dorofeyeva1, G.G. Vorobyov1, I.Y. Cherepaha1, A.I. Kornelyuk2, V.F. Sagach1
- Bogomoletz Institute of Physiology, National Academy of
Sciences of Ukraine, Kyiv
- The Institute of Molecular Biology and Genetics, National
Academy of Sciences of Ukraine, Kyiv
DOI: https://doi.org/10.15407/fz63.06.003
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Abstract
To investigate the effects of EMAP II on cardiac function and
endothelium-dependent relaxation of smooth muscles of the
aorta in diabetes mellitus, studies were conducted on 6 monthsold
male streptozotocin-induced diabetic rats and control rats.
In experiments in vivo, we have shown that EMAP II improves
diastolic heart function in streptozotocin-induced diabetic rats.
The End-diastolic pressure in streptozotocin-induced diabetic
rats was decreased by 25,4% (P<0,05), the end-diastolic myocardial
stiffness decreased by 25,5% (P<0,05). After EMAP II
the end-systolic pressure decreased by 13,8% and the arterial
stiffness decreased by 26,3% (P<0,05). In vitro experiments on II promotes the improvement and restoration of endotheliumdependent
relaxation in streptozotocin-induced diabetic rats.
Inhibition of NOS activity with L-NAME abolished EMAP
II-mediated vasodilatation in the endothelium-intact aorta that
indicates the NOS-dependent mechanism of action of EMAP
II. Thus, EMAP II had a positive effect on the heart and vessels
of rats with streptozotocin-induced diabetes, improving
diastolic heart function and vasorelaxation in diabetes.
Keywords:
Endothelial monocyte-activating polypeptide II (EMAP II); diabetes; endothelium-dependent relaxation of the aorta; heart
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