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Fiziologichnyi Zhurnal - <a id="lj1_ret_bk_to_jl0_href_id" href="?list0=10">Fiziol. Zh. 2008;</a> <a id="lj1_ret_bk_to_jl0_href_id" href="?list1=49">54(4):</a> 47-54
Українська Русский English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2008; 54(4): 47-54


Effect of cord blood cells on interferonogenesis and effector cells of immune system

L.M. Lazarenko, O.M. Demchenko, L.T. Rachkova, L.D. Kryvokhats’ka, Z.M. Olevins’ka, M.Ya. Spivak

    D.K. Zabolotny Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Kyiv, Ukraine


Abstract

Human cord blood cells were shown to produce interferon-α- and -γ in vitro, both spontaneously and following adequate induction. Transplantation of human blood cord cells to mice led to endogenous interferonogenesis activation, serum interferon levels as well as interferon-α and interferon-γ synthesis by activated peripheral blood leucocytes becoming higher. The maximal interferon accumulation in murine blood sera following transplantation did not coincide with interferonogenesis dynamics observed due to the effect of standard early and late interferon inducers poly I:C and ridostin, respectively. Interferonogenesis activation was accompanied by increased functional activty of phagocytic system cells.

Keywords: Blood cells, smmune system

References

    1. Гулевський О.К., Грищенко В.І., Нікольченко А.М., Моісєєва Н.М. Властивості і перспективи викорис- тання кордової крові в клінічній практиці // Укр. журн. гематології та трасфузології. 2005. № 5. С.5-14. 2. Кузовкова Н.А. Оценка активности естественных киллеров колориметрическим методом // Иммуно- логия. 1991. № 4. С. 59-61. 3. Кравчук Б.О., Спивак Н.Я., Стинич О.А и др. Состояние системы интерферона при обструктивном бронхите у детей раннего возраста с синдромом гиперплазии тимуса // Совр. педиатрия. 2004. L.M. L 4. Кухарчук А.Л., Радченко В.В., Сирман В.М. Стволовые клетки: эксперимент, теория, клиника. К: КРС-Медицинские Технологии, 2004. 505 с. 5. Современные методы диагностики вирусных респираторных инфекций и их терапии с исполь- зованием препаратов интерферона (методические рекомендации) / Под. ред. Модзолевского А. Ф., Дяченко Н. С., Спивака Н. Я. К., 1994. 18 с. 6. Стинич О.А. Порушення показників системи імунітету та інтерферону при гіперплазії тимусу у дітей з обструктивним бронхітом: Атореф. дис. ... канд.мед.наук. К., 2003. 19 с. 7. Деклараційний патент UA62221U на корисну модель Спосіб виділення «стовбурових» та клітин-попоред- ників гемопоезу пуповинної крові людини (Гладких Ю.В., Любимцева Г.С., Калиниченко Т.О.) 15.04. 2005, Бюл. винаходів №4. 2005 р. 8. Deng W., Han Q., Liao L. et al. Effects of allogeneic bone marrow-derived mesenchymal stem cells on T and B lymphocytes from BXSB mice // Cell. Biol. 2005. 24, № 7. P. 458-463. 9. Gur H., Krauthgamer R., Bachar-Lusting E. et al. Immune regulatory activity of CD34+ progenitor cells: evidence for a deletion-based mechanism mediated by TNF-alpha // Blood. 2005. 105, № 6. P. 2585-2593. 10. Gotherstrom C., Ringder O., Tammik C. et al. Immunologic properties of human fetal mesenchymal stem cells // Amer. J. Obstet. Gynecol. 2004. 190, № 1. P. 239-245. 11. Huang Y.J., Sun Q.Y., Liu L.H. et al. Kinetic study of various cytokine mRNA expression in rhesus treated with haploidentical peripheral blood stem cell transplantation // Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2006. 14, № 3. P.571-576. 12. Jin Y. H., Rhee Y.G., Su Y.S. et al. Cotransplantation of cord blood hematopoietic ctem cells and culture-expanded and GM-CSF-/SCF-transfected mesenchymal stem cells in SCID mice // J. Koren. Med. Sci. 2007. 22, № 2. P. 242-247. 13. Krampera M., Cosmi L., Angeli R. et al. Role for interferon-gamma in the immunomodulatory activity of human bone marrow mesenchymal stem cells // Stem Cells. 2006. 24, № 2. P. 386-398. 14. Klyushnenkova E., Mosca J.D., Zernetkina V. et al. T cell responses to allogeneic human mesenchymal stem cells: immunogeniciyu, tolerance, and suppression // J. Biomed. Sci. 2005. 12, № 1. P. 47-57. 15. Kim J.A., Hong S., Lee B. et al. The inhibition of Tcells proliferation by mouse mesenchymal stem cells through the induction of p161NK4A-cyclin D1/cdk and p21 walf, p27kip1-cyclin E/cdk2 pathways // Cell Immunol. 2007. 245, № 1. P. 16-23. 16. Kang H.S., Habib M., Chan J. et al. A paradoxical role for IFN-gamma in the immune properties of mesenchymal stem cells during viral challenge // Exp. Hematol. 2005. 33, № 7. P. 796-803. 17. Lin C., Chen S., Yang L. et al. Evaluation of TCR V beta subfamily T cell expansion in NOD/SCID mice transplanted with human cord blood hematopoietic stem cells // Hematology. 2007. 12, № 4. P. 235-230. 18. Li H., Guo Z.K., Li X.S. et al. Functional and phenotypic alteration of intrasplenic lymphocytes affected by mesenchymal stem cells in a murine allosplenocyte transfusion model // Cell Transplantant. 2007. 16, № 1. P. 85-95. 19. Oikawa A., Ito K., Seguchi H. et al. Development of immunocompetent lymphocytes in vivo from murine umbilical cord blood cells// Transplantation. 2007. 84, № 1. P. 23-30. 20. Pommey S., Galipeau J. The use of mesenchymal stromal cells in oncology and cell // Bull Cancer. 2006. 93, № 9. P. 901-907. 21. Spaggiari G.M., Capobianco A., Becchetti S., Mingari M.C., Moretta L. Mesenchymal stem cell-natural killer cell interaction: evidence that activated NK cells are capable of killing MSCs, whereas MSCs can inhibit IL- 2-induced NK-cell proliferation // Blood. 2006. 107, № 4. P. 1484-1490. 22. Wang Y., Chen X., Armstrong M.A., Li G. Survival of bone marrow-derived mesenchymal stem cells in xenotransplantation model // J. Orthop. Res. 2007. 25, № 7. P. 926-932.

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