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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2017; 63(5): 55-61

Melatonin deficiency or excess and various-genesis stressful situations influence on liver functioning

O.I. Antonova, А.V. Pasenko

    Kremenchuk Mykhailo Ostrohradskyi National University, Ukraine


The paper deals with the research of the functional, pro- and antioxidant condition of the liver under the impact of stressful situation of various geneses. The markers of the oxidizing stress included: diethen conjugates, xanthine oxidase activity, cyclic adenosine monophosphate. The state of the liver pro- and antioxidant system was assessed by the increase of the peroxidation secondary oxoproducts mainly malondialdehyde (MDA) under the conditions of spontaneous and induced (ascorbate-depending and enzymatic) peroxidation and also by the activity of superoxide dismutase and catalase, content of restored and oxidized forms of glutathione in the liver. General proteolytic activity in the liver tissue was determined. Total bilirubin and its fractions were determined according to the standard method of Jendrashek with the use of diazoreagent. It was found out that the action of aggravators causes increase of non-enzymatic free-radical peroxide oxidation of biopolymers. It turned out that at the lack of melatonin the content of diene conjugate increases by 30% but there was a tendency to decrease of the content of MDA by 30%. Thus, it is observed that only the concentration of primary peroxidation products increase, which may certify the intensification of peroxidation in the liver. At the impact of increased doses of melatonin the peroxidation processes in the liver reduced, which is proved by half as big content of MDA, i.e. melatonin acted as an antioxidant. The research of the liver functional state under the conditions of the oxidizing stress made it possible to identify in the liver twice as high content of diethen conjugates and MDA in comparison with the control value, which characterize the peroxidation processes.Thus, a conclusion can be made that stressful situations cause intensification of peroxidation in the liver, which may contribute to various diseases. Key words:

Keywords: Stress; liver; pro- and antioxidant balance.


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