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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2017; 63(5): 55-61

Melatonin deficiency or excess and various-genesis stressful situations influence on liver functioning

O.I. Antonova, А.V. Pasenko

    Kremenchuk Mykhailo Ostrohradskyi National University, Ukraine


The paper deals with the research of the functional, pro- and antioxidant condition of the liver under the impact of stressful situation of various geneses. The markers of the oxidizing stress included: diethen conjugates, xanthine oxidase activity, cyclic adenosine monophosphate. The state of the liver pro- and antioxidant system was assessed by the increase of the peroxidation secondary oxoproducts mainly malondialdehyde (MDA) under the conditions of spontaneous and induced (ascorbate-depending and enzymatic) peroxidation and also by the activity of superoxide dismutase and catalase, content of restored and oxidized forms of glutathione in the liver. General proteolytic activity in the liver tissue was determined. Total bilirubin and its fractions were determined according to the standard method of Jendrashek with the use of diazoreagent. It was found out that the action of aggravators causes increase of non-enzymatic free-radical peroxide oxidation of biopolymers. It turned out that at the lack of melatonin the content of diene conjugate increases by 30% but there was a tendency to decrease of the content of MDA by 30%. Thus, it is observed that only the concentration of primary peroxidation products increase, which may certify the intensification of peroxidation in the liver. At the impact of increased doses of melatonin the peroxidation processes in the liver reduced, which is proved by half as big content of MDA, i.e. melatonin acted as an antioxidant. The research of the liver functional state under the conditions of the oxidizing stress made it possible to identify in the liver twice as high content of diethen conjugates and MDA in comparison with the control value, which characterize the peroxidation processes.Thus, a conclusion can be made that stressful situations cause intensification of peroxidation in the liver, which may contribute to various diseases. Key words:

Keywords: Stress; liver; pro- and antioxidant balance.


  1. Baraboi VA, Reznikov AG. The physiology, biochemistry and psychology of stress. Kyiv, Interservis. 2013; 314. [Ukrainian].
  3. Bellommo G. Mechanisms of oxidative cell injury. Actaphysiol. scand. Suppl. 1992; 146 (608): 28.
  5. Belenichev IF, Gubskii YuI, Levitskii EL, Kovalenko SI. The regulation of the antioxidant homeostasis and the system of the body detoxication by melatonin hormone. The role of the melatonin-dependent receptors in the realization of this function. Modern probl toxicol [electronic resource]. 2003; 2. st 2003/03_2_2/htm.
  7. Pashkov aN. Melatonin influence on the oxidative status, the content of citrate and the activity of aconitate hydratase in rats' liver at toxic hepatitis. Probl endocrinol. 2005; 48(6): 41-43.
  9. Rudyi-Trypolskyi VO. The morphological structure of white rats' liver under stressful condition on the background of the hypo- and hyper-function of the epiphysis. Morph conf. Dnipropetrovsk. 2006; 86. [Ukrainian].
  11. Popov SS. The clinical efficiency of melatonin and its impact on the free radical homeostatis at the toxic lesion of the liver. Med Sc Cand thesis. Voronezh. 2008; 25. [Russian].
  13. Petrishchev NN, Kvetnoi IM, Panchenko AV, Anisimov VN.  The length of the day light phase as a factor modulating the colon carcinogenesis in the experiment . Conf 50 years of melatonine: research results and prospects. St. Petersburg. 2008; 30-2. [Russian].
  15. Koroliuk Ma, Ivanova LI, Maiorova IG, Tokarev VE. A method for determination of catalase activity. Laboratory business. 1988; 1:16-19.
  17. Syrota TV. A new approach to the research of adrenaline autooxidation process and its use for measuring the activity of superoxide dismutase. Probl med chem. 1999; 45(3): 263-72.
  19. Kaidashev IP. Methods of clinical and experimental research in medicine. Poltava. Polymet. 2003; 319. [Ukrainian].
  21. Tsebrzhynskyi OI, Neporada KS, Denysenko SV. Sources of superoxide generation at an acute stress. Topical probl modern med. 2002; 2; 2(4): 42-4. [Ukrainian].
  23. Tsebrzhynskyi OI, Kostenko aH, Kostenko Va. Stress features under the conditions of intoxication and inflammation. Physiol jour. 1998; 44(4): 89. [Ukrainian].
  25. Shkurupii Va. Ultrastructure of the liver cells in stress. Novosibirsk. Nauka. 1989; 144. [Russian].
  27. Brechko VB, Yeromina OL, Tsebrzhynskyi OI. Influence of different types motion modes on the antioxidant status in the experiment depending on the season and the type of higher nervous activity. Experimental and clinical physiology and biochemistry. 1999; 2:7-10.
  29. Antonova OI. Influence on the liver of light and partial deprivation of sleep. J Med biol probl. 2006; 1:18-22. [Ukrainian].
  31. Antonova OI, Tsebrzhynskyi OI. Chronic hyper-melatonin condition influence on the state of rats' liver. J Luhansk Taras Shevchenko National Pedagogic University. 2006; 13(108): 6-10. [Ukrainian].

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