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Melatonin deficiency or excess and various-genesis stressful situations influence on liver functioning
O.I. Antonova, А.V. Pasenko
Kremenchuk Mykhailo Ostrohradskyi National University, Ukraine
DOI: https://doi.org/10.15407/fz63.05.055
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Abstract
The paper deals with the research of the functional, pro- and antioxidant condition of the liver under the
impact of stressful situation of various geneses. The markers of the oxidizing stress included: diethen conjugates,
xanthine oxidase activity, cyclic adenosine monophosphate. The state of the liver pro- and antioxidant
system was assessed by the increase of the peroxidation secondary oxoproducts mainly malondialdehyde
(MDA) under the conditions of spontaneous and induced (ascorbate-depending and enzymatic) peroxidation
and also by the activity of superoxide dismutase and catalase, content of restored and oxidized forms of
glutathione in the liver. General proteolytic activity in the liver tissue was determined. Total bilirubin and
its fractions were determined according to the standard method of Jendrashek with the use of diazoreagent.
It was found out that the action of aggravators causes increase of non-enzymatic free-radical peroxide oxidation
of biopolymers. It turned out that at the lack of melatonin the content of diene conjugate increases
by 30% but there was a tendency to decrease of the content of MDA by 30%. Thus, it is observed that
only the concentration of primary peroxidation products increase, which may certify the intensification of
peroxidation in the liver. At the impact of increased doses of melatonin the peroxidation processes in the
liver reduced, which is proved by half as big content of MDA, i.e. melatonin acted as an antioxidant. The
research of the liver functional state under the conditions of the oxidizing stress made it possible to identify
in the liver twice as high content of diethen conjugates and MDA in comparison with the control value,
which characterize the peroxidation processes.Thus, a conclusion can be made that stressful situations cause
intensification of peroxidation in the liver, which may contribute to various diseases.
Key words:
Keywords:
Stress; liver; pro- and antioxidant balance.
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