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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2017; 63(5): 28-33


INFLUENCE OF MELANINE ON MORPHOFUNCTIONAL INDICES OF RAT SKIN WITH CHEMICAL BURNER AND FULL-THICKNESS WOUND

O.V. Taburets, V.V. Vereschaka, T.V. Beregova, L.I. Ostapchenko

    Education and Research center «Institute of Biology» Taras Shevchenko National University of Kyiv
DOI: https://doi.org/10.15407/fz63.05.028

Abstract

On white nonlinear male rats (200-250 g), we investigated the content of moisture, collagen, melted gelatin and the temperature of welding in the skin of animals with full-thickness wounds and chemical burn, without treatment and under the treatment by a new pharmacological melanin-containing composition. The physical and chemical indices of the skin were examined on white nonlinear male rats (the full-thickness wound was simulated with a stencil and surgical tweezers, chemical burn, by subcutaneous injection with 0.1 ml (10%) of CaCl2. Moisture content was reduced by 36% in a wound, during chemical burn by 48%. In addition, we observed an increase in the collagen content by 40 and 52%, respectively. When correcting the pathology by melanin, the normalization of the investigated parameters was detected. As tissue scarring does not occur, the gelatin yield decreases in the cut wounds by 40 %, and the skin welding temperature decreases. Тhe melanin composition that we created can be recommended as a dermatotropic agent for the treatment of skin wounds of various origins.

Keywords: full-thickness wound; chemical burn; collagen; melanin.

References

  1. Guo S, Dipietro L. Factors affecting wound healing. J Dent Res. 2010 Mar; 89 (3):219-29. CrossRef PubMed PubMedCentral
  2.  
  3. Cheret J, Lebonvallet N, Carre J, Misery L. Influence of sensory neuropeptides on human cutaneous wound healing process. J Dermatol Sci. 2014; Jun 74(3):193-203. CrossRef PubMed
  4.      
  5. Hamed S, Bennett C.L, Demiot C, Desmouliere A. Erythropoietin, a novel repurposed drug: an innovative treatment for wound healing in patients with diabetes mellitus. Wound Repair Regen. 2014;22(1):23-33. CrossRef PubMed
  6.      
  7. Cetin EO, Yesil-Celiktas O, Cavusoglu T. Incision wound healing activity of pine bark extract containing topical formulations: A study with histopathological and biochemical analyses in albino rats. Pharmazie. 2013;68:75-80. PubMed
  8.  
  9. Sun BK, Siprashvili Z, Khavari PA. Advances in skin grafting and . treatment of cutaneous wounds. Science. 2014;346:941-5. CrossRef PubMed
  10.  
  11. Richmond NA, Lamel SA, Davidson JM, Martins-Green M, Sen CK, Tomic-Canic M, Vivas AC, Braun LR, Kirsner RS. US–National Institutes of Health-funded research for cutaneous wounds in 2012. Wound Repair Regen. 2013;21:789-92. [PubMed]. CrossRef PubMed
  12.  
  13. Nayak BS, Anderson M, Pinto Pereira LM: Evaluation of wound-healing potential of Catharanthus roseus leaf extract in rats. Fitoterapia. 2007;78:540-4. CrossRef PubMed
  14.  
  15. Taburets OV, Morgaienko OO, Kondratiuk TO, Beregova TV, Ostapchenko LI. The Effect of «Melanin-Gel» on the Wound Healing. RJPBCS. 2016;7(3):2031-8.
  16.  
  17. Garg VK, Paliwal SK. Wound-healing activity of ethanolic and aqueous ex- tracts of Ficus benghalensis. J Adv Pharm Technol Res. 2011;2:110-4. CrossRef PubMed PubMedCentral
  18.      
  19. Maxson S, Lopez EA, Yoo D et al. Concise review: Role of mesenchymal stem cells in wound repair. Stem Cells Transl Med. 2012;1:142-9. CrossRef PubMed PubMedCentral
  20.      
  21. Bilyayeva O, Neshta V, Golub A, Sams-Dodd F. Effects of SertaSil on wound healing in the rat. J Wound Care. 2014 Aug;23(8):140-6. CrossRef PubMed
  22.  
  23. Okhmat OA, Plavan VP, Mokrousova OP, Danilkovich AG. Chemical analysis and physical and mechanical tests of skin and fur: methodological instructions for laboratory work on the discipline «Analytical control in fur and leather production''. KNUTD. Kiev. 2006; 60-4. [Ukrainian].
  24.  
  25. Vereshchaka VV. Physico-chemical properties of the collagen of the skin of healthy people of all ages and people with symptoms of senile sagging. Dermatol and Venereol. 2007; 4 (38):12-8. [Ukrainian].
  26.  
  27. Mavrova DI. Metall maintenance and physical-chemical indices of unaffected skin site under chronic dermatosis. Dermatol and Venereol. 2009;3(45):62-6.
  28.  
  29. Marik PE, Flemmer M. The immune response to surgery and trauma: Implications for treatment. J Trauma Acute Care Surg. 2012;73(4):801-8. CrossRef PubMed
  30.  

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