EFFECT OF AGMATINE ON ACTIN POLYMERIZATION DYNAMICS IN WGA-STIMULATED LEUKOCYTES UNDER DIABETES MELLITUS IN RATS
I.V. Brodyak, I.I. Bila, N.O. Sybirna
Ivan Franko National University of Lviv, Ukraine
DOI: https://doi.org/10.15407/fz63.04.048
Abstract
We examined the effect of agmatine on the aggregation ability
of white blood cells induced by sialоspecific lectin of wheat
germ (WGA) and the process of polymerization of actin
cytoskeleton of leukocytes in normal conditions and under
conditions of experimental diabetes mellitus (DM) after 0.5, 1
and 3 min of preincubation of these cells with the WGA. Our
studies revealed that agmatine administration to animals with
DM leading to a 2.7- and 3.2-fold increase of the aggregate
size and aggregation velocity of leukocytes, respectively,
compared to diabetic rats who did not receive this polyamine
injections. The achieved results can testify to the increase of
sialoglycoconjugates and N-acetyl-D-glucosaminecontaining
glycans on leukocyte surface. The leukocytes of animals with
DM introduced on the background of agmatine insertion that
were activated by WGA-stimulatory signals contain more
polymerized actin in comparison with the cells of control
animals on the background of this polyamine insertion as
with leukocytes of animals with DM. Our data suggest
that under conditions of DM the agmatine insertion makes
the recovery of affinity binding of complementary ligands
with sialoglycoconjugates with subsequent intracellular
transduction of lectin-induced signal which causes the
reorganization of the actin cytoskeleton in leukocytes.
Keywords:
polymerised actin; WGA-induced aggregation; sialic acid; leukocytes; agmatine; experimental diabetes mellitus.
References
- Badr G, Bashandy S, Ebaid H, Mohany M, Sayed D. Vitamin C supplementation reconstitutes polyfunctional T cellsin streptozotocin-induced diabetic rats. Eur J Nutr. 2012;51:623-33.
CrossRef
PubMed
- Lehuen A, Diana J, Zaccone P, Cooke A. Immune cell crosstalk in type 1 diabetes. Nature Rev Immun. 2010;10:501-13. doi:10.1038/nri2787.
CrossRef
- Ma J, Hart GW. Protein O-GlcNAcylation in diabetes and diabetic complications. Expert Rev Proteomics. 2013; 10(4): 365-80.
CrossRef
PubMed PubMedCentral
- Ferents IV, Brodyak IV, Lyuta MYa, Burda V A, Gavrylyshyn GS, Sybirna NO. Effect of agmatine on the blood system parameters of rats under the condition of experimental diabetes mellitus. Studia Biologica. 2012;6(3):65-72. [Ukrainian].
- Hwang SL, Liu IM, Tzeng TF, Cheng JT. Activation of imidazoline receptors in adrenal gland to lower plasma glucose in streptozotocin-induced diabetic rats. Diabetologia. 2005;48(4):767-75.
CrossRef
PubMed
- Piletz JE, Aricioglu F, Cheng J-T, Fairbanks CA, Gilad VH, Haenisch B, et al. Agmatine: clinical applications after 100 years in translation. Drug Discov Today. 2013;18(17- 18):880-893.
- Lee C-L, Chiu PCN, Pang P-C, Chu IK, Lee K-F, Koistinen R, et al. Glycosylation Failure Extends to Glycoproteins in Gestational Diabetes Mellitus. Diabetes. 2011;60:909-17.
CrossRef
PubMed PubMedCentral
- Gloster TM, Vocadlo DJ. Developing inhibitors of glycan processing enzymes as tools for enabling glycobiology. Nature Chem Biol. 2012;8:683-94.
CrossRef
PubMed
- Liu K, Liu HY, Ye W, Jiang JH, Xu X. The initial investigation of the expression of glycosyltransferases in the retina of streptomycin diabetic rats. Zhonghua Yan Ke Za Zhi. 2010;46(7):580-4.
PubMed
- Sybirna NO, Shevtsova a?, Ushakova GO, Brodyak IV, Pismenetzka ?Y. Fundamentals of glycobiology. Monograph; Lviv: Ivan Franko National University of Lviv; 2015. [Ukrainian].
- Mocsai A, Walzog B, Lowell CA. Intracellular signalling during neutrophil recruitment. Cardiovasc Res. 2015; 107(3):373-85.
CrossRef
PubMed PubMedCentral
- Scott DW, Patel RP. Endothelial heterogeneity and adhesion molecules N-glycosylation: Implications in leukocyte trafficking in inflammation. Glycobiology. 2013;23(6):622-33.
CrossRef
PubMed
- Vestweber D. How leukocytes cross the vascular endothelium. Nature. 2015;15:692-704.
CrossRef
- Green CE, Pearson DN, Camphausen RT, Staunton DE, Simon SI. Shear-dependent capping of L-selectin and P-selectin glycoprotein ligand 1 by E-selectin signals activation of high-avidity 2-integrin on neutrophils. J Immunol. 2004;172:7780-90.
CrossRef
PubMed
- Oliveira S, Rosowski EE, Huttenlocher A. Neutrophil migration in infection and wound repair: going forward in reverse. Nature. 2016;16:378-91.
CrossRef
- Luo J, Xu T, Wang X, Ba X, Feng X, Deepak V, et al. PI3K is involved in L-selectin- and PSGL-1-mediated neutrophil rolling on E-selectin via F-actin redistribution and assembly. J Cell Biochem. 2010;110(4):910-9.
CrossRef
PubMed
- Fenteany G, Glogauer M. Cytoskeletal remodeling in leukocyte function. Curr Opin Hematol. 2003;11:15-24.
CrossRef
- Alonso-Lebrero JL, Serrador JM, Domínguez-Jiménez C, Barreiro O, Luque A, del Pozo MA, et al. Polarization and interaction of adhesion molecules P-selectin glycoprotein ligand 1 and intercellular adhesion molecule 3 with moesin and ezrin in myeloid cells. Blood. 2000;95(7):2413-9.
PubMed
- Lin W-L, Guu S-Y, Tsai C-C, Prakash E, Viswaraman M, Chen H-B, et al. Derivation of cinnamon blocks leukocyte attachment by interacting with sialosides. PLoS ONE. 2015; 10(6):e0130389.
CrossRef
PubMed PubMedCentral
- Lapovets LE, Lutsyk BD. Handbook of Laboratory Immunology, Lviv, 2002. [Ukrainian].
- Ferents I, Brodyak I, Lyuta M, Klymyshyn N, Burda V, Sybirna N. Sialylation status of leukocyte cell-surface glycoconjugates in streptozotocin-induced diabetic rats and after treatment with agmatine. Curr Issues Pharmacy and Med Scien. 2013;26(4):390-2.
CrossRef
- Brodyak IV, Bila II, Sybirna NO. Influence of WGAstimulatory signals on actin polymerization in leukocytes of streptozotocin-induced diabetic rats. Visnyk of Lviv University. Biol ser. 2016; 73:35-40. [Ukrainian].
- Carulli G, Mattii L, Azzarà A, Brizzi S, Galimberti S, Zucca A, et al. Actin polymerization in neutrophils from donors of peripheral blood stem cells: divergent effects of glycosylated and nonglycosylated recombinant human granulocyte colony-stimulating factor. Am J Hematol. 2006;81(5):318-23.
CrossRef
PubMed
- Brodyak IV, Bila II, Overchuk M, Sybirna NO. Effect of agmatine on actin polymerization in leukocytes of streptozotocin-induced diabetic rats. Studia Biologica. 2014;8(3-4):17-30.
|