The male rats’ bile acids compositions of bile under the experimental chronic social stress conditions were investigated. It was found that the concentration of conjugated bile acids in rat bile was significantly lower in stressed animals than in the control group. Taurocholic acid concentration in the bile of stressed animals were 23,4 – 23,6 %) lower than in the control group. Chenodeoxycholic and deoxycholic taurine conjugates concentration in stressed animals bile was 18,9 – 23,14 % lower than in the control group. The concentration of the bile acids conjugated with glycine in stressed male rats’ bile was lower than in the control group too (glycocholic acid – 32,9 – 51,3 % lower than the control values, and the concentration of glycochenodeoxycholic and glycodeoxycholic acids – 33,8 – 55,7 % lower than the control values). The concentration of free cholic acid in stressed male rats’ bile was also lower than the control values 33,7 – 48,2 %. But in stressed animals bile samples chenodeoxycholic and deoxycholic acids concentrations was 19,8 – 25 % higher than in control samples of bile. The most significant difference in bile acids content of the male rats with chronic social stress bile and in the bile of the control group rats was in the emergence of the lithocholic and taurolithocholic acids in the hepatic secret of stressed animal. We can propose that in the chronic social stress conditions in the male rat liver processes of synthesis, biotransformation and transport of bile acids into the bile were inhibited. The role of chronic social stress in disorders of bile formation and the role of bile acids as bioactive regulatory compounds in occurrence and progression of stress-induced pathologies are require a fundamental research.