Українська Русский English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2017; 63(4): 24-29


INFLUENCE OF EXPERIMENTAL CHRONIC SOCIAL STRESS ON BILE ACIDS CONTENT IN THE BILE OF MALE RATS

A.M. Liashevych, I. I. Tubalceva, Ye.M. Reshetnik, O.V. Bondarenko, S.P. Veselsky, M.Yu. Makarchuk

    Kyiv National Taras Shevchenko University, Ukraine
DOI: https://doi.org/10.15407/fz63.04.024

Abstract

The male rats’ bile acids compositions of bile under the experimental chronic social stress conditions were investigated. It was found that the concentration of conjugated bile acids in rat bile was significantly lower in stressed animals than in the control group. Taurocholic acid concentration in the bile of stressed animals were 23,4 – 23,6 %) lower than in the control group. Chenodeoxycholic and deoxycholic taurine conjugates concentration in stressed animals bile was 18,9 – 23,14 % lower than in the control group. The concentration of the bile acids conjugated with glycine in stressed male rats’ bile was lower than in the control group too (glycocholic acid – 32,9 – 51,3 % lower than the control values, and the concentration of glycochenodeoxycholic and glycodeoxycholic acids – 33,8 – 55,7 % lower than the control values). The concentration of free cholic acid in stressed male rats’ bile was also lower than the control values 33,7 – 48,2 %. But in stressed animals bile samples chenodeoxycholic and deoxycholic acids concentrations was 19,8 – 25 % higher than in control samples of bile. The most significant difference in bile acids content of the male rats with chronic social stress bile and in the bile of the control group rats was in the emergence of the lithocholic and taurolithocholic acids in the hepatic secret of stressed animal. We can propose that in the chronic social stress conditions in the male rat liver processes of synthesis, biotransformation and transport of bile acids into the bile were inhibited. The role of chronic social stress in disorders of bile formation and the role of bile acids as bioactive regulatory compounds in occurrence and progression of stress-induced pathologies are require a fundamental research.

Keywords: liver; bile; bile acid; chronic social stress

References

  1. Baraboi VA, Reznikov OH. Physiology, biochemistry and psychology of stress. Monograph. 2013. [Ukrainian]. PubMed PubMedCentral
  2.  
  3. Harasymiv IM. Effect of cold stress on liver function zhovchoutvoryuvalnu experiment. J Res. 2014;1:96-7. [Ukrainian].
  4.  
  5. Shkurashivska SV, Ersteniuk HM. Dynamics of lipid metabolism in tissues and organs of experimental animals under conditions of stress adrenaline. Med Clin Chem. 2015;17(4):34-7. [Ukrainian]. CrossRef  
  6. Zahaiko AL, Voronina LM, Kaliman PA, Strelchenko KV. Influence of chronic social stress on lipid metabolism in golden Syrian hamsters. Ukr Biochem J. 2008;80(4):120-9. [Ukrainian].
  7.  
  8. Chuang J-C, Cui H, Mason L. Chronic social defeat stress disrupts regulation of lipid synthesis. J Lipid Res. 2010.1344-53.
  9.  
  10. Scott KA, Melhorn SJ, Sakai RR. Effects of Chronic Social Stress on Obesity. Curr Obes Rep. 2012;1(1):16-25. CrossRef PubMed PubMedCentral
  11.  
  12. Chiang JY. Bile Acid Metabolism and Signaling. Compr Physiol. 2013;3(3):1191–1212. CrossRef  
  13. Zhao C, Dahlman-Wright C. Liver X receptor in cholesterol metabolism. J Endocrinol. 2010;204:233-240. CrossRef PubMed
  14.  
  15. Vasheka IP, Veselskyi SP, Horenko ZA, Hrinchenko OA, Karbovska LS, Makarchuk MYu. Impact on amilinu range holativ bile in rats. Fiziol Zh. 2014;60(3):46-53. [Ukrainian]. PubMed
  16.  
  17. Norlin M, Wikvall K. Enzymes in the conversion of cholesterol into bile acids. Curr Mol Med. 2007;7(2):199-218. CrossRef PubMed
  18.  
  19. Trauner M, Claudel T, Fickert P, Moustafa T, Wagner M. Bile acids as regulators of hepatic lipid and glucose metabolism. Dig Dis. 2010;28(1):220-224. CrossRef PubMed
  20.  
  21. Fuchs C, Claudel T, Trauner M. Bile acid-mediated control of liver triglycerides. Semin Liver Dis. 2013;33(4):330-42. CrossRef PubMed
  22.  
  23. Zhou H, Hylemon PB. Bile acids are nutrient signaling hormones. Steroids. 2014;86:62-68. CrossRef PubMed PubMedCentral
  24.  
  25. Pierre JF, Martinez KB, Ye H. Activation of bile acid signaling improves metabolic phenotypes in high-fat dietinduced obese mice. Physiol Gastrointest Liver Physiol. 2016;311(2):286-304. CrossRef PubMed PubMedCentral
  26.  
  27. Maleszka A, Dumnicka P, Matuszyk A. The Diagnostic Usefulness of Serum Total Bile Acid Concentrations in the Early Phase of Acute Pancreatitis of Varied Etiologies. Int J Mol Sci. 2017;18(1). CrossRef  
  28. European convention for protestion of vertebrate animals used for experimental and other scientific purpose. Book European convention for protestion of vertebrate animals used for experimental and other scientific purpose. Editor Strasbourg, 18.03.1986.
  29.  
  30. The Law of Ukraine "On protection of animals from cruelty" from 21.02.2006 number 3447-IV. Supreme Council of Ukraine. 2006;27:230.
  31.  
  32. Kudryavtseva N. A sensory contact model for the study of aggressive and submissive behavior in male mice. Aggressive Behavior. 1991;17(5):285-91. CrossRef  
  33. Method for determining zhelchnuh acids in the fluid Biology: A.s.4411066 /14USSR, MBY G01 N33/50/SP Veselskyi, PS Liashchenko, YA Lukianenko. N1624322.
  34. Filimonova NB, Fil IO, Mykhailova TS. Statistical analysis of the data according to the principles of science-based medicine. Initial analysis of quantitative data, experimental results presentation. Medcine Railway Transport of Ukraine. 2004;4:30-8. [Ukrainian].
  35. Filimonova NB, Fil IO. Statistical analysis of the data according to the principles of science-based medicine. Comparison groups in quantitative terms. Med Transport Ukr. 2005;4:86-93. [Ukrainian].
  36.  
  37. Boyer JL. Bile Formation and Secretion. Comp. Physiol. 2013;3(3):1035-78. CrossRef  
  38. Sjöberg BG. Studies on cholesterol and bile acid metabolism in relation to plasma lipoproteins. THESIS FOR DOCTORAL DEGREE (Ph.D.). Stockholm, 2016. ISBN978-91-7676-299-8.
  39.  
  40. Matsubara T, Tanaka N, Patterson AD, Cho J-Y, Krausz KW, Gonzalez FJ. Lithocholic acid disrupts phospholipid and sphingolipid homeostasis leading to cholestasis. Hepatology. 2011;53(4):1282-93. . CrossRef PubMed PubMedCentral

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2019.