Українська English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2017; 63(4): 17-23

NGF - INDUCED CHANGES IN ADRENERGIC MODULATION OF CALCIUM CHANNELS OF TRIGEMINAL GANGLION NEURONS

M.V. Telka, O.V. Rikhalsky, N.S. Veselovsky

    O.O. Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz63.04.017


Abstract

We investigated the influence of nerve growth factors (NGF) on adrenergetic modulation voltage dependent calcium currents (VDCC) in trigeminal ganglion neurons. Noradrenalin had no effect on low VDCC but inhibited the high VDCC. Application of noradrenalin (100 µM) resulted in decrease amplitude of high VDCC by (30±5)% and (13±8)% under with or without addition of NGF in the culture medium respectively. When NGF was administered in the TG cell cultures, noradrenalin depressed fast component of high VDCC in majority of neurons (11 of 13).Only in three units of 15 were observed changes in the kinetics of high VDCC under condition without NGF. The changes in the kinetics of high VDCC were accompanied by shifting steady-state inactivation curves to more negative values. These data showed that high voltage dependent calcium channels are involved in adrenergic modulation in TG neurons. In the presence of NGF this effect is enhanced. Our researches were suggest the increase levels of NGF contributed to effect on adrenergic modulation.

Keywords: trigeminal ganglion neurons; voltage dependent calcium channels; noradrenalin; nerve growth factor.

Full text: (PDF, in Ukrainian)

References

  1. Devor M, Janig W, Michaelis M. Modulation of activity in dorsal root ganglion neurons by sympathetic activation in nerve-injured rats. J Neurophysiol. 1994; 71: 37-47. CrossRef PubMed
    2.  
  2. Sato J, Perl ER. Adrenergic excitation of cutaneous pain receptors induced by peripheral nerve injury. Science. 1991; 251:1608-10. CrossRef PubMed
    3.  
  3. Marchetti C, Carbone E, Lux HD. Effect of dopamine and noradrenaline on Ca channels of cultured sensory and sympathetic neurons of chick. Pfluger Arch. 1986; 406 :104-11. CrossRef  
  4. Yagi J, Sumino R. Inhibition of a hyperpolarizationactivated current by clodine in rat dorsal root ganglion neurons. J Neurophysiol. 1998 Sep; 80(3): 1094-104. CrossRef PubMed
    5.  
  5. Takeda M, Ikeda M, Tanimoto T, Lipski J and Matsumoto S. Changes of the excitability of rat trigeminal root ganglion neurons evoked by alpha2-adrenoreceptors. Neurosci. 2002; 115(3):731-41. CrossRef  
  6. Soeda H, Tatsumi H, Katayama Y. Neurotransmitter release from groth cones of rat dorsal root ganglion neurons in culture. Neurosci. 1997; 77:1187-99. CrossRef  
  7. Thayer SA, Miler RJ. Regulation of the intracellular free calcium concentration in single rat dorsal root ganglion neurons in vitro. J Physiol. 1990; 425: 85-115. CrossRef  
  8. Abdulla FD, Smith PA. Ectopic alpha2-adrenoceptors couple to N-Type Ca channels in axotomized rat sensory neurons. J Neurosci. 1997 March; 17(5): 1633-41. PubMed
    9.  
  9. Honma Y, Yamakage M, Ninomiya T. Effects of adrenergic stimulus on the activities of Ca 2 and K+ channels of dorsal root ganglion neurons in neuropathic pain model. Brain Research. 1999; 832: 195-206. CrossRef  
  10. Levi-Montacini R, Angelett PU. Nerve growth factor. Physiol Rev. 1968; 48: 534-69. CrossRef PubMed
    11.  
  11. Ritter AM, Lewin GR, Kremer NE, Mendell LM. Requirement for nerve growth factors in the development of myelinated nociceptors in vivo. Nature. 1991; 350: 500-2. CrossRef PubMed
    12.  
  12. Bennet DL, Averill S, Clary DO, Priestley JV, Mcmahon SB. Postnatal changes in the expression of the trkA highaffinity NGF receptors in primary sensory neurons. Eur J Neurosci. 1996; 8:2204-8. CrossRef  
  13. Fang X, Djouhri L, McMullan S, Berry C, Okuse K, Waxman SG et al. TrkA is expressed in nociceptive neurons and influences electrophysiological properties via Nav1.8 expression in rapidly conducting nociceptors. J Neurosci. 2005 May 11; 25(19):4868-78. CrossRef PubMed
    14.  
  14. Ueda M, Hirose M, Takei N, Ibuki T, Naruse Y, Amaya F et al. Nerve growth factor induces systemic hyperalalgesia after thoracic burn injury in the rat. Neurosci Lett. 2002; 328:97-100. CrossRef  
  15. Woolf CJ, Safieh-Garabedian B, Ma QP, Crilly P, Winter J. Nerve growth factor contributes to the generation of inflammatory sensory hypersensitivity. Neurosci. 1994; 62:327-31. CrossRef  
  16. Shu X, Mendell LM. Acute sensitization by NGF of the response of small-diameter sensory neurons to capsaicin. J Neurophysiol. 2001 Dec; 86:2931-8. CrossRef PubMed
    17.  
  17. Zhu W,Galoyan SM, Petruska JC, Oxford GS, Mendell LM. A developmental switch in acute sensitization of small dorsal root ganglion neurons to capsaicin or noxious heating by NGF. J Neurophysiol. 2004 Nov; 92: 3148-52. CrossRef PubMed
    18.  
  18. Kitamura N, Konno A, Kuwahara T, Komagiri Y. Nerve growth factor induced hyperexcitability of rat sensory neuron in culture. Biomedical Research. 2005; 26(3): 123-30. CrossRef PubMed
    19.  
  19. Djouhri L, Dawbarn D, Robertson A, Newton R, Lawson SN. Time course and nerve growth factor dependence of inflamation-induced alteratios in electrophysiological membrane properties in nociceptive primary afferent neurons. J Neurosci. 2005 Nov 15; 21(22): 8722-33.
    20.  
  20. Zhang Q, Tan Y. Nerve growth factor augments neuronal responsiveness to noradrenaline in cultured dorsal root ganglion neurons of rats. Neurosci. 2011;193:72-9. CrossRef PubMed
    21.  
  21. Tal M, Devor M. Ectopic discharge in injured nerves: comparison of trigeminal and somatic afferents. Brain Research. 1992; 579:148-51. CrossRef  
  22. Veselovsky NS, Engert F, Lux HD. Fast local superfusion technique. Pfluger Arch. 1996; 432: 351-4. CrossRef  
  23. Telka MV, Rikhalsky OV, Veselovsky NS. Excitability properties of trigeminal ganglion neurons, Fiziol. Zh. 2016; 62(2): 24-34. CrossRef

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2024.