Changes in amplitudes of evoked inhibitory postsynaptic currents (eIPSCs) from rat cultured hippocampal neurons were studied using whole-cell patch-clamp technique in postsynaptic neuron and local extracellular electrical paired pulse stimulation of single presynaptic axon. Both paired pulse facilitation (PPF) and paired pulse depression (PPD) were observed in postsynaptic currents in response to paired pulse stimulation of single presynaptic axon with interstimulus interval (ISI) from 20 ms to 1200 ms. In most neurons stimulation induced depression (n = 12). The longer interstimulus interval, the less depression was observed. Maximum depression of second eIPSC was during the shortest ISI (20 ms) with paired pulse ration (PPR) equal 42 ± 6 %. Minimum depression of second eIPSC was during the longest interstimulus interval (1200 ms) and PPR was equal 98 ± 6 %. Rate recovery from PPD is well described by a single exponential function having time constant of 83 ms. PPF was maximal at an ISI of 150 ms (PPR equal 118 ± 4 %) and recovered within 500 ms (n = 6). Rate recovery from PPF has bell-shape form. In 7 neurons we observed depression of amplitude of eIPSC after consecutive stimulations of single presynaptic axon by four stimulus at ISI ranging from 50 ms to 200 ms. The relation between normalized amplitude and frequency of stimulation well fitted by exponential function with time constant ranged from 41 ms (ISI = 50 ms) to 119 ms (ISI = 200 ms).