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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2017; 63(4): 3-9


А.Yu. Luchkova, Yu.V. Hoshovska, R.A. Fedichkina, N.А. Strutynska, V.F. Sagach

    O.O. Bogomoletz Institute of Physiology of the National Academy of Sciences of Ukraine, Kyiv, Ukraine


The present study was aimed to investigate the effect of О-carboxymethyl hydroxylamine (O-CMH) on heart function in conditions of Ca2+ loads and mitochondrial permeability transition pore (MPTP) opening in cardiac mitochondria. OCMH (50 mg per kg) was dissolved in physiological solution and injected intraperitoneally 30 min before the experiment. Rat isolated hearts were Langendorf-perfused and subjected to increased concentration of Ca2+ in perfusion solution ranged from 1.7 to 12.5 mmoles/L. The heart function was assessed by measuring the LVDP, dP/dt, the coronary flow, the heart rate. The opening of MPTP was estimated by monitoring Ca2+ induced mitochondria swelling at 520 nm. The results showed that pretreatment with O-CMH significantly depressed the initial contractile activity of the isolated rat hearts and the coronary flow. Further modeling of Ca2+ loads was accompanied with lower increment of LVDP and dP/dt comparing to control rats indicating decreased functional reserves and low effectiveness of Ca2+ management in O-CMH pretreated rats. Additionally, cardiac mitochondria in O-CMH group were more sensitive to Ca2+ showing maximum swelling at 10-5 moles/L in the incubation medium vs 10-4 moles/L in control group (p<0.05). Pre-incubation of cardiac mitochondria with O-CMH in concentration of 10-5, 10-4 and 10-3 moles/L leaded to increased Ca2+ induced swelling by 17.8, 20 and 44% respectively. Thus, we have found that inhibition of mitochondrial H2S synthesis pathway increase sensitivity of cardiac mitochondria to Ca2+ that lead to mitochondrial swelling and decreased ability of myocardium to manage Ca2+ homeostasis by cardiomyocytes in conditions of Ca2+ load. This might be proposed as the physiological role of H2S synthesized in mitochondria by 3-MPST. Key words: .

Keywords: Hydrogen sulfide; calcium; MPTP; MPST; mitochondria; heart


  1. Guo W, Kan J, Cheng Z, Chen J, Shen Y, Xu J, Wu D, Zhu Y. Hydrogen Sulfide as an Endogenous Modulator in Mitochondria and Mitochondria Dysfunction. Ox Med and Cell Long. 2012; 1-9.
  3. Olson KR. The therapeutic potential of hydrogen sulfide: separating hype from hope. Am J Physiol Regul Integr Comp Physiol. 2011; 301:297-312. CrossRef PubMed
  5. Kuo MM, Kim DH, Jandu S, Bergman Y. Tan S, Wang H, Pandey DR, Abraham TP, Shoukas AA, Berkowitz DE, Santhanam L. MPST but not CSE is the primary regulator of hydrogen sulfide production and function in the coronary artery. Am J Physiol Heart Circ Physiol. 2016; 310:H71-9 CrossRef PubMed PubMedCentral
  7. Dedkova EN, Blatter LA. Mitochondrial Ca2+ and the heart. Cell Calcium. 2008; 44:77-91. CrossRef PubMed
  9. Glancy B, Balaban RS. Role of Mitochondrial Ca2+ in the Regulation of Cellular Energetics. Biochemistry. 2012; 51:2959-73. CrossRef PubMed PubMedCentral
  11. Halestrap AP, Pasdois Ph. The role of the mitochondrial permeability transition pore in heart disease. Biochimica et Biophysica Acta. 2009; 1787:1402-15. CrossRef PubMed
  13. Sagach VF, Shimanskaya TV, Nadtochiy SM. Factor that is released during the reperfusion of ischemic heart can be a marker of mitochondrial pore opening. Fiziol Zh. 2003; 49(4):6-12. [Ukrainian].
  15. Sagach VF, Vavilova GL, Strutyns'ka NA, Rudyk OV. The aging increase in the sensitivity of the mitochondrial permeability transition pore opening to inductors in rat heart. Fiziol Zh. 2004; 50(2): 49-63. [Ukrainian].
  17. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; (1):265-75. PubMed
  19. Shymans'ka TV, Hoshovs'ka IuV, Semenikhina OM, Sahach VF. Effect of hydrogen sulfide on isolated rat heart reaction under volume load and ischemia-reperfusion]. Fiziol Zh. 2012; 58(6):57-66. [Ukrainian]. PubMed
  21. Shimanskaia TV, Strutinskaia NA, Vavilova GL, Goshovskaia IuV, Semenikhina EN, SagachVF. Cyclosporin A-sensitive mitochondrial pore as a target of cardioprotective action of hydrogen sulfide donor. Rus J of Physiol. 2013 Feb; 99(2):261-72. [Russian].
  23. Vassalle M, Lin Ch-I. Calcium Overload and Cardiac Function. J Biomed Sci. 2004; 11:542-565. CrossRef PubMed
  25. Strutynska NA, Kotsiuruba AV, Budko AYu, Mys LA, Sagach VF. Mitichondrial dysfunction in the aging heart is accompanied by constitutive NO-synthases uncoupling on the background of oxidative and nitrosative stress. Fiziol Zh. 2016; 62(2):3-11. CrossRef  
  26. Baumgartner HK, Gerasimenko JV, Thorne Ch, Ferdek P, at all. Calcium Elevation in Mitochondria Is the Main Ca2+ Requirement for Mitochondrial Permeability Transition Pore (mPTP) Opening. J of Biol Chem. 2009; 284(31):20796-803. CrossRef PubMed PubMedCentral
  28. Strutynska NA, Semenykhina OM, Chorna SV, Vavilova GL, Sagach VF. Hydrogen sulfide inhibits Ca2+-induced mitochondrial permeability transition pore opening in adult and old rat heart. Fiziol Zh. 2011; 57(6):3-14. [Ukrainian].

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