Українська Русский English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2015; 61(5): 21-27


Influence of single nucleotide polymorphisms of vitamin D receptor-gene on the level of osteoassociated hormones linkage with postmenopausal osteoporosis

D.S. Ziablitsev, O.S. Larin

    Ukrainian Science-Practical Centre of Endocrine Surgery, Transplantation of Endocrine Organs and Tissues Ministry of Healthcare of Ukraine, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz61.05.021

Abstract

The levels of osteoassociated hormones, macroelements, interleukines, cyclic nucleotides and activity of alkaline phosphatase were being detected in the blood plasma of 74 postmenopausal women with and without osteoporosis (OP). Bone mineral density (BMD) and the metacarpal index were being assessed according to the results of densitometry. The allele polymorphisms vitamin D receptor gene (VDR) Cdx2 (rs11568820) and TaqI (rs731236) were being identified with the help of the polymerase chain reaction. The major genotype CC TaqI was found in the group without OP 3.4 times more often than with OP, while the minor genotype TT was found 3.4 times more often with the presence of OP. The presence of the genotype CC TaqI decreased the risk of OP progress by 5.5 times, and the genotype TT TaqI increased by 5.4 times. The presence of the minor allele T was associated with the higher levels of interleukin 1β, BMD and lower testosterone blood level. The major homozygote AA Cdx2 was found in women without OP 2.9 times more often, while the minor genotype GG – 5.4 times more often with the presence of OP. The presence of the major homozygote decreased the risk of OP progress by more than 5.3 times, while the minor homozygote increased the risk by 8 times. The presence of the minor allele G in the genotype was associated with the increase of parathyroid hormone, phosphorus, magnesium, alkaline phosphatase activity in blood, BMD and with the decrease of testosterone, progesterone and calcium blood levels.

Keywords: postmenopausal osteoporosis: hormones: VDR: polymorphism Cdx2 (rs11568820): polymorphism TaqI (rs731236)

References

  1. Kulakov VI, Manukhin IB, Soloveva Gynecology: National Guidness. 2011: 848. [Russian].
  2.  
  3. Yakushevskaya OV, Yureneva SV. Pathogenetic bases of the sharp phase development of the response to intravenous administration of nitrogen-containing bisfosfonat. Obstetrics and Gynecol. 2013;5: 19-23. [Russian].
  4.  
  5. Mishchenko EB. Importance assessment of the external and internal risk factors of osteoporosis development. Intern Endocrinol J. 2011. 1 (33): 15-21. [Russian].
  6.  
  7. Bischoff L., Derk CT. Premenopausal osteoporosis. Minerva Medica J. 2008: 99 (1): 55-63.
  8.  
  9. Povoroznyuk VV, Masik NP, Pirogova NI, 2015. Bone tissue markers and their clinical value at a chronic obstructive pulmonary disease. Health of Kazakhstan 202/33:50-8. [Russian].
  10.  
  11. Haussler MR, Whitfield GK, Kaneko I. Molecular mechanisms of vitamin D action. Calcified Tissue Internat. 2013. 92 (2): 77-98.
  12.  
  13. Schunack W. Vitamin D3 a prodrug of different D3- hormones. 101 Suppl 1: Medizinische Klinik (Munich, Germani). 2006, 20-24: 20-4.
  14.  
  15. Li WF, Hou SX, Yu B, Li MM. Genetics of osteoporosis: accelerating pace in gene identification and validation. Human Genetics. 2010. 127 (3): 249-285.
  16.  
  17. Casado-Díaz A, Cuenca-Acevedo R, Navarro-Valverde C. Vitamin D status and the Cdx-2 polymorphism of the vitamin D receptor gene are determining factors of bone mineral density in young healthy postmenopausal women. Molecul Biol. 2012: 0960 (12): 192–6.
  18.  
  19. Baig L, Mansuri FA, Karim SA. Association of menopause with osteopenia and osteoporosis: results from population based study done in Karachi. J Coll Physic and Surg Pakistan. 2009; 19 (4): 240-4.
  20.  
  21. Jia F, Sun RF, Li QH, Wang DX, Zhao F, Li JM, Pu Q, Zhang ZZ, Jin Y, Liu BL, Xiong Y. Genetical Testing of Molecular Biomarkers. 2015; 30-34.
  22.  
  23. Xu H, Li S, Qiu JQ, Gao XL, Zhang P, Yang YX. The VDR gene Fok I polymorphism and ovarian cancer risk. Tumour Biol: 2013; 3309-16.
  24.  
  25. Ziablitsev DS, Pishchulina SV, Kishenya MS, Chernobrivtsev AP. Value of vitamin D receptor gene polymorphisms in the pathogenesis of osteodeficiency of postmenopausal period women. Morph Bull. 2014; 2 (20): 432-5. [Russian].

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2019.