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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2015; 61(5): 11-20


Proteasomal activity in brain tissue following ischemic stroke in Wistar rats

O.I. Savchuk1, M.O. Orlovsky1, Ie.S. Iarmoliuk2, S.V. Goncharov1, V.E. Dosenko1, G.G. Skibo1

  1. О.О. Bogomoletz Institute of Physiology of National Academy of Science of Ukraine, Kyiv, Ukraine
  2. О.О. Bogomolets National Medical University, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz61.05.011

Abstract

Functional as well as structural reorganization of brain tissues takes place in the surrounding and remotes brain areas after focal ischemic lesions. In particular, reactive or regenerative processes have been described to occur in the infarction areas and the contralateral hemisphere. Experiments were performed on 63 rats, divided into 3 groups (each consisted of 21 animals): sham operated, short-term occlusion of the right middle cerebral artery (MCAO) group, and long-term MCAO group. We have studied changes in proteasome proteolysis during transient occlusion of the middle cerebral artery using method of Koizumi J., duration 2 and 60 min and made the comparison between changes in different types of proteasome activity and severity of ischemic injury and showed three types of decrease in proteolytic activity (trypsin-, chymotrypsin-like, peptidylglutamyl peptide-hydrolyzing) in the brain tissues. Chymotrypsin-like activity of ischemic areas of the brain for short-term MCAO decreased 4.1 times compared with controls (P>0.05), for long-term MCAO decreased 5.8 times compared with controls (P<0.05). Trypsin-like activity of ischemic areas of brain for short-term MCAO decreased 7.1 times compared with controls (P>0.05), for long-term MCAO decreased 12.5 times compared with controls (P<0.05). PGPH activity of ischemic areas for shortterm MCAO decreased 8 times compared with controls (P>0.05), for long-term MCAO decreased 2.8 times compared with controls (P<0.05). The similar dynamics was observed also in the penumbra and the core zone of the brain at 6 h of reperfusion, in the long run there is no significant difference between the core and contralateral zones. Our results suggest that proteasome activity may play also a role in contralateral cortical plasticity occurring after focal cerebral ischemia.

Keywords: ischemic stroke; focal cerebral ischemia; proteasome

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