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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 1999; 45(5): 55-59

The in-vitro resumption of meiotic maturation and of the first polar body by mouse oocytes at different stages of the estrous cycle

Aleksieieva IM, Voznesens'ka TIu, Blashkiv TV, Ianchiĭ RI.

    A. A. Bogomolets Institute of Physiology, National Academy of Ukraine, Kiev.


Resumption of the meiotic maturation and extruded the first polar body of the murine oocytes was registered at 2 and 14 hours under culturing in the medium containing 1.7 mM Ca2+. There were differences between the groups in the percentage of GV oocytes with zona pellucida that acquired competence to undergo germinal vesicle breakdown (GVBD) at 2 hr time point 22.2% of "small" and 32.4% of "large" follicles of mouse at dioestrus and, respectively, 28.1% and 41.8% follicles of mouse at oestrous underwent GVBD. At 14 time point 21.4% of "small" and 30.3% of "large" follicles of mice at mice at dioestrus and, respectively, 26.1% and 43.6% follicles of mouse at oestrous underwent GVBD and (then) extruded the first polar body (FPB). It may be suggested, that acquisition of competence of the oocytes to resume meiosis in vitro depends on the size of follicles and the stage of murine oestrous cycle. Thus, the largest percentage of GV oocytes resuming meiosis was from "largest" follicles of mouse at oestrous.


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