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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2001; 47(5): 41-46


The mechanism of nitric oxide effects on the smooth musclecontractile apparatus ca2+-sensitivity of force development

O.Y. Andruchov, V.F. Sagach

    A.A.Bogomoletz Institute Physiology UkrainianAcademy of Sciences, Kiev


Abstract

It was investigated whether the SH-groups of con- tractile proteins are involved in NO-induced relaxation of saponin-skinned smooth muscle strips. Both, the thiol-specific alkylating agent N-ethylmaleamide (NEM), and thiol-reducig agent dithiotreitol (DTT) induced relaxation of maximally activated skinned rat portal vein preparations. The relaxing effects of DTT and nitric oxide donor sodium nitroprusside were not addіtive. After the relaxation, induced by one of this agent, the effect of the other one was negligible. We suggest that NO-induced smooth muscle relaxation be due to decreasing of force sensitivity to Ca2+. This effect of nitric oxide is realized by its interaction with critical thiol groups of smooth muscle contractile proteins.

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