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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2017; 63(2): 73-79


A.K. Gulevsky, E.S. Abakumowa, I.I. Shenyavsky

    Institute for problems of cryobiology and cryomedicine of the NAS of Ukraine, Kharkov.


We investigate the biological activity of low molecular weight (<5 kDa) fraction, obtained by ultrafiltration from cord and peripheral blood from cows and dairy calves. It is shown that the injection of fraction obtained from cows cord blood and dairy calves blood significantly increased the resistance to hypoxia and physical endurance in experimental mice. A higher ratio of survival time and restitution time (respectively, 7.4±0.7 and 6.9±0.8 vs. 5.2±0.7 in control) demonstrates significant (P<0.05) antihypoxic action of these fractions. The results obtained after the preceding five-day course of injections proved the antihypoxant properties of low molecular fraction from cord blood and from dairy calves blood. The duration of mice swimming with aggravating load, which before the tests were given injections of low-molecular fraction from cord blood and from dairy calves blood, was increased each day and on the fourth day. The increase has already been reliable not only in relation to the control group of animals data’s (P<0.05), but also to the data’s of the relevant group on the first day of the experiment (P<0.05). The low molecular weight fraction obtained from blood оf adult cows did not show positive effects in any tests. Based on the obtained results it is concluded that being high in the early stages of ontogenesis the biological activity of blood low-molecular fraction (up to 5 kDa) decreases with age.

Keywords: fraction to 5 kDa of hemodialysates; cord blood; аctovegin; biological activity; ontogenesis; resistance to hypoxia; physical endurance


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