BIOLOGICAL ACTIVITY OF LOW MOLECULAR WEIGHT FRACTION OBTAINED FROM CORD AND PERIPHERAL BLOOD IN COWS OF DIFFERENT AGES
A.K. Gulevsky, E.S. Abakumowa, I.I. Shenyavsky
Institute for problems of cryobiology and cryomedicine of
the NAS of Ukraine, Kharkov.
We investigate the biological activity of low molecular weight
(<5 kDa) fraction, obtained by ultrafiltration from cord and
peripheral blood from cows and dairy calves. It is shown that
the injection of fraction obtained from cows cord blood and
dairy calves blood significantly increased the resistance to
hypoxia and physical endurance in experimental mice. A higher
ratio of survival time and restitution time (respectively, 7.4±0.7
and 6.9±0.8 vs. 5.2±0.7 in control) demonstrates significant
(P<0.05) antihypoxic action of these fractions. The results
obtained after the preceding five-day course of injections
proved the antihypoxant properties of low molecular fraction
from cord blood and from dairy calves blood. The duration
of mice swimming with aggravating load, which before the
tests were given injections of low-molecular fraction from
cord blood and from dairy calves blood, was increased each
day and on the fourth day. The increase has already been
reliable not only in relation to the control group of animals
data’s (P<0.05), but also to the data’s of the relevant group on
the first day of the experiment (P<0.05). The low molecular
weight fraction obtained from blood оf adult cows did not show
positive effects in any tests. Based on the obtained results it is
concluded that being high in the early stages of ontogenesis
the biological activity of blood low-molecular fraction (up to
5 kDa) decreases with age.
fraction to 5 kDa of hemodialysates; cord blood; аctovegin; biological activity; ontogenesis; resistance to hypoxia; physical endurance
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