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EFFECT OF GERMANIUM CITRATE ON THE IMMUNOPHYSIOLOGICAL ACTIVITY IN RATS
R. S. Fedoruk, M. I. Khrabko, O. P. Dolaychuk
Institute of Animal Biology NAAS, Lviv
DOI: https://doi.org/10.15407/fz63.02.065
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Abstract
We examined the effect of germanium citrate on immunophysiological
mechanisms of homeostatic reactions of rats
of different ages and physiological states. We show that daily
exposure of female rats to germanium citrate obtained by nanotechnology (groups 2-4) and chemical synthesis (groups
5-6) in the doses of 10 (group 2), 20 (group 3), 200 (group 4 and
5) and 2000 (group 6) mcg/kg resulted in immunophysiological
changes in animals of all research groups. In the blood
of female rats after 4 months of germanium intake, and
infant male rats of groups 5 and 6 at 60-65 days of life, the
concentration of immunoglobulins significantly increased by
1.73 – 2.45 times (females) and 1.05 – 1.1 times ( males). The
concentration of circulating immune complexes increased by
1.12 – 3.10 and 1.27– 2.02 times, sialic acids by 1.01 – 1.54
and 1.39 – 1.59 times; hexoses bound to proteins by 1.12 –
1.17 and 1.20 – 1.33 times in groups 5 and 6. In all rats, the
level of average molecular weight was reduced by 6 – 17
%, platelet count by 22 – 39 % in females and 6 – 23 % in
males, and phagocytic activity by 5.3 – 10.2% and 4.4 –
5.7%, respectively, which points to the same direction of the
immunobiological action of germanium citrate in adult and
young rats of both sexes. We also found a reduction in the
concentration of ceruloplasmin and the number of white blood
cell in the blood of female rats of research groups 3 – 4 and
their growth in males of groups 2– 4. Studying the state of the
immune system and content of glycoproteins in the blood, we
analyzed the immunophysiological characteristics of influence
of low and high doses of germanium citrate depending on the
duration of its intake, as well as systemic communication of
the mother and fetus. The data indicate for the sex and age
specific features of hepato- immunological communication
in the organism of these animals under prolonged germanium
citrate load. The biological effect is more pronounced in rats
treated with 20 and 200 mcg of germanium.
Keywords:
immunophysiology; rats; Germanium citrate; blood; glycoproteins; immunoglobulins; hematological data; nanomaterials
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© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2024.
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