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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2017; 63(2): 46-55


DEPENDENCE OF THE ENDOCRINE FUNCTION OF OVARIAN TISSUE TRANSPLANT AFTER ISCHEMIA ON THE INTENSITY OF LIPID PEROXIDATION

V.V. Kiroshka1, Yu.O. Bozhkova1, I.A. Trutaieva1, A.A. Gawas2

  1. Institute for Problems of Cryobiology and Cryomedicine, Kharkiv;
  2. V.N. Karazin Kharkiv National University
DOI: https://doi.org/10.15407/fz63.02.046

Abstract

The correlation between endocrine function, morphology and volume changes in the structure of ovarian tissue, as well as intensity of lipid peroxidation at different temperature and time parameters after ischemia modeling were investigated. It was shown that the morphological transformations after 2 hours of ischemia at 37°C and 24 at 4°C are reversible. In this case, a significant increase in the concentration of sex hormones in animals-recipients relative to the castrated animals after heterotopic transplantation was observed. An increase in time of ischemia up to 4 hours at 37°C and 48 hours at 4°C led to morphological transformation and irreversible failure of endocrine function. This was not observed when the tissue was incubated (4°C, 48 hours) in a mannitol-containing solution, where the level of products of the thiobarbituric acid accumulation was 2.5-3 times lower, and correlated with a significant increase in the estradiol and progesterone concentrations in recipient animals. Thus, the combined protective effect of hypothermia and mannitol at the ischemia conditions was demonstrated.

Keywords: ovarian tissue; ischemic damage; endocrine function; the volume of oocytes.

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