The correlation between endocrine function, morphology and volume changes in the structure of ovarian tissue, as well as intensity of lipid peroxidation at different temperature and time parameters after ischemia modeling were investigated. It was shown that the morphological transformations after 2 hours of ischemia at 37°C and 24 at 4°C are reversible. In this case, a significant increase in the concentration of sex hormones in animals-recipients relative to the castrated animals after heterotopic transplantation was observed. An increase in time of ischemia up to 4 hours at 37°C and 48 hours at 4°C led to morphological transformation and irreversible failure of endocrine function. This was not observed when the tissue was incubated (4°C, 48 hours) in a mannitol-containing solution, where the level of products of the thiobarbituric acid accumulation was 2.5-3 times lower, and correlated with a significant increase in the estradiol and progesterone concentrations in recipient animals. Thus, the combined protective effect of hypothermia and mannitol at the ischemia conditions was demonstrated.