ACTIVATION OF TRPV1 BY NITRIC OXIDE DONORS REQUIRES CO-APPLICATION OF SULFHYDRIL-CONTAINING REAGENT
B.R. Sharopov, Y.M. Shuba
O.O. Bogomoletz Institute of Physiology, National Academy
of Sciences of Ukraine, Kyiv
DOI: https://doi.org/10.15407/fz63.02.003
Abstract
Previous studies suggested that polymodal capsaicin-sensitive
TRPV1 ion channel could also be a receptor for nitric oxide
(NO). However, the evidence for this notion is based on
rather indirect experiments, which questions its physiological
relevance. The present paper provides an improved method
of application of NO donors such as sodium nitroprusside
(SNP) in an electrophysiological experiment, implying the
simultaneous delivery of sulfhydryl-containing reagents such
as L-cysteine (L-Cys) that facilitates the release of NO from
a donor substance and allows inducing macroscopic TRPV1-
mediated current. We found that external application of 100 μM
SNP per se onto dorsal root ganglia (DRG) neurons responsive
to capsaicin cannot activate TRPV1 ion channel. In contrast,
the application of SNP simultaneously with 100 μM L-Cys
induces transmembrane current with features characteristic
of TRPV1-mediated one, i.e., inward at command membrane
potential (Vcomm) +50 mV, outward at Vcomm -100 mV, and
susceptible to blockade with capsazepine, a selective TRPV1
antagonist. At the same time, the application of L-Cys itself
evoked no response in conditions tested. To summarize, our
work confirms the assumption about the sensitivity of TRPV1
to NO and provides a simple method for further studies of
their interaction.
Keywords:
TRPV1; nitric oxide; NO donors; L-cysteine; patch clamp
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