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THE EFFECT OF OF POLY(ADP-RIBOSE) POLYMERASE INHIBITOR 4-HYDROXYQUINAZOLINE ON DEATH OF IMMUNE CELLS UNDER IMMUNE COMPLEX-MEDIATED INJURY IN MICE
N.G. Grushka
О.О. Bogomoletz Institute of Physiology of the National
Academy of Sciences of Ukraine, Kyiv
DOI: https://doi.org/10.15407/fz63.01.043
Abstract
The influence of poly(ADP-ribose) polymerase (PARP) inhibitor
4-hydroxyquinazoline (4-HQ) on the level of DNA
damage and on the death of thymic and lymph node cells in
mouse model of immune complex injury was investigated to
reveal its possible cytoprotective effect. As shown by comet
assay, DNA damage index of immune cells was increased
4,0 times in mice with immune complex-mediated pathology
induced by a long-term immunization of CBA mice with
bovine serum albumin (BSA), Р<0,001. The percentage of
thymic cells with strong DNA damage was increased to 77 %
under immunization (compared to 1,5 % in control mice) and
the percentage of such cells from lymph nodes was increased
to 80 % (compared to 0 % in control), in both cases P< 0,001.
Genotoxic stress was reduced by treatment of immunized mice
with 4-HQ: the percentage of lymphocytes with strong DNA
damage was significantly decreased that promoted increase
in the amount of cells having intact DNA. PARP inhibition
exerted a strong cytoprotective effect: viability of thymus and
lymph node cells was increased mainly due to reduced level of
necrosis. So, our results suggest that PARP may be involved
in thymic and lymph node cell damage in immune complex
mediated pathology and give evidence that inhibition of this
enzyme may constitute a perspective target in immune complex
diseases prevention and therapy.
Keywords:
https://10.15407/fz63.01.043
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