AGE AND SEX FEATURES OF BONE MINERAL DENSITY IN RATS
N.V. Grygorieva1, V.V. Povoroznyuk1, I.V. Gopkalova2, T.V. Orlyk1
- State Institution «D. F. Chebotarev Institute of Gerontology
NAMS Ukraine», Kyiv;
- State Institution «V. Danilevsky Institute of Endocrine
Pathology Problems NAMS Ukraine», Kharkіv
In vivo assessment of bone mineral density (BMD) and bone
mineral content (BMC) indices gives an objective description
of the possible rates of bone loss during aging and various
diseases, as well as response to treatment by various drugs,
affecting bone metabolism. This study was conducted on 376
Wistar rats of both genders, divided into 12 age groups using
the method of dual-energy X-ray absorptiometry. Studying the
parameters of BMD and BMC depending on age and sex has
found no bone mass «peak» in rats of both sexes. Bone mineral
density indices were increased in both male and female rats
during growing, more pronounced in males. These differences
are noted in all age groups of animals. Moreover, we have
established age and gender particularities of BMC indices
in different parts of the skeleton. In female rats, a significant
increase of BMD and BMC parameters have been detected
in age from 1.5 to 2 months (33 %), from 2 to 3 months – by
12.5 %. In males, the increase of BMD indices was found to
happen 1 month later and it was less pronounced (from 2 to
3 months – 27 %, from 4 to 5 months – 5 %). In other age
periods, the dynamics of BMD indices had only an upward
trend, but significant differences different groups were not
established. Our results of BMD and BMC indices and their
age and gender features can be used for further research in
osteology as reference data for modeling of experimental
osteoporosis in rats and study of response to the use of different
rat bone mineral density; bone mineral content; bone area; age; sex.
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