Українська English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2017; 63(1): 17-25

Probiotic strains of lactobacilli and bifidobacteria alter pro- and anti-inflammatory cytokines production in rats with monosodium glutamate-induced obesity

T.M. Falalyeyeva1, I.V. Leschenko1, T.V. Beregova1, L.M. Lazarenko1,2, O.M. Savchuk1, L.M. Sichel2, O.I. Tsyryuk1, T.B. Vovk1, M.Ya. Spivak2,3

  1. Taras Shevchenko National University of Kyiv, Kyiv, Ukraine;
  2. D.K. Zabolotny Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Kyiv, Ukraine;
  3. LCL «Diaprof», Kyiv, Ukraine


The aim of this study was to investigate the effect of probiotic strains of Lactobacillus casei IMV B-7280, Bifidobacterium animalis VKL, B. animalis VKB on the pro- and anti-inflammatory cytokines production in Wistar male rats with monosodium glutamate (MSG)-induced obesity. It was established that neonatal administration of MSG to rats leads to increasing levels of the interleukin (IL)-1β and IL-12, and to decreasing of the IL-4, IL-10 and tumor growth factor (TGF)-β levels in the blood serum. After administration of the B. animalis VKL - B. animalis VKB - L. casei IMV B-7280 composition to obese rats the level of the IL-1β in blood serum wasn’t differ from that in the obese rats, that didn’t receive of the probiotic bacteria. But there was no statistically significant difference comparing with intact rats. The level of the IL-12B p40 in blood serum was decreased under influence of the B. animalis VKL - B. animalis VKB - L. casei IMV B-7280 composition (18.9 %, p < 0.05) and B. animalis VKL (10.5 %, p < 0.05) compared with obese rats, not receiving probiotic bacteria, but remained higher than in intact animals. After administration to obese rats of the B. animalis VKL - B. animalis VKB - L. casei IMV B-7280 composition the levels of the IL-4, IL- 10 and TGF- β increased in blood serum comparing with obese rats, not receiving probiotic bacteria. The level of the IL-10 also increased under influence of the B. animalis VKB, and IL-4 – under influence of the L. casei IMV B-7280. Our results suggest that these probiotic bacteria and probiotic composition are able to down-regulation the inflammation in rats with MSG-induced obesity but the strongest anti-inflammatory effects have probiotic composition. The ability of lactobacilli and bifidobacteria to alter the pro- and antiinflammatory cytokines production, opens perspectives to create new treatments for obesity and metabolic syndrome based on probiotics.

Keywords: obesity; monosodium glutamate; Lactobacilli; Bifidobacteria; cytokines; rats.


  1. Alberti KG, Zimmet P, Shaw J. The metabolic syndrome – a new worldwide definition. Lancet. 2005; 366 (9491): 1059–62
  2. CrossRef
  3. Boulangé CL, Neves AL, Chilloux J, Nicholson JK, Dumas ME. Impact of the gut microbiota on inflammation, obesity, and metabolic disease. Genome Med. 2016; 8 (1): 42.
  4. CrossRef
  5. Esposito Emanuela, Anna Iacono, Giuseppe Bianco, Autore G, Cuzzocrea S, Vajro P, et al. Probiotics reduce the inflammatory response induced by a high-fat diet in the liver of young rats. J. Nutr. 2009; 139 (5): 905–11.
  6. CrossRef
  7. Bastard JP, Maachi M, Lagathu C, Kim MJ, Caron M, Vidal H, et al. Recent advances in the relationship between obesity, inflammation, and insulin resistance. Eur Cytok Network. 2006; 17 (1): 4–12.
  8. Velloso LA, Araújo EP, de Souza CT. Diet-induced inflammation of the hypothalamus in obesity. Neuroimmunomodulation. 2008; 15 (3):189–93.
  9. CrossRef
  10. Dallman MF, la Fleur SE, Pecoraro NC, Gomez F, Houshyar H, Akana SF. Minireview: glucocorticoids-food intake, abdominal obesity, and wealthy nations in 2004. Endocrinology. 2004; 145 (6): 2633–38.
  11. CrossRef
  12. Deng T, Lyon CJ, Minze LJ, Lin J, Zou J, Liu JZ, et al. Class II major histocompatibility complex plays an essential role in obesity-induced adipose inflammation. Cell Metab. 2013; 17 (3): 411–22.
  13. CrossRef
  14. Glantz Stanton A., Primer of Biostatistics. 4th ed., Mc- Graw-Hill Inc., New York; 1997. Lee B-C, Lee J. Cellular and molecular players in adipose tissue inflammation in the development of obesity-induced insulin resistance. Biochim Biophys Acta. 2014; 1842 (3): 446–62.
  15. CrossRef
  16. Esser N, Legrand-Poels S, Piette J, Scheen AJ, Paquot N. Inflammation as a link between obesity, metabolic syndrome and type 2 diabetes. Diabet Res Clinical Pract. 2014; 105 (2): 141–50.
  17. CrossRef
  18. Jung SH, Park HS, Kim KS, Choi WH, Ahn CW, Kim BT, et al. Effect of weight loss on some serum cytokines in human obesity: increase in IL-10 after weight loss. J Nutr Biochem. 2008; 19 (6): 371–75.
  19. CrossRef
  20. Musso G, Gambino R, Cassader M. Obesity, diabetes, and gut microbiota: the hygiene hypothesis expanded? Diabet Care. 2010; 33 (10): 2277–84.
  21. CrossRef
  22. Gomez-Merino D., Drogou C., Guezennec C.Y., Chennaoui M. Effects of chronic exercise on cytokine production in white adipose tissue and skeletal muscle of rats. Cytokine. 2007; 40 (1): 23–9.
  23. CrossRef
  24. Guijarro A, Laviano A, Meguid MM. Hypothalamic integration of immune function and metabolism. Prog Brain Res. 2006; 153: 367–405.
  25. CrossRef
  26. Aggarwal J, Swami G, Kumar M. Probiotics and their Effects on Metabolic Diseases: An Update. J Clin Diagn Res. 2013; 7(1): 173–77.
  27. CrossRef
  28. Lazarenko L, Babenko L, Sichel LS, Pidgorskyi V, Mokrozub V, Voronkova O, et al. Аntagonistic action of Lactobacilli and Bifidobacteria in relation to Staphylococcus aureus and their influence on the immune response in cases of intravaginal staphylococcosis in mice. Probiot Antimicr Proteins. 2012; 4 (2):78–89
  29. CrossRef
  30. Lim SM, Jeong JJ, Woo KH, Han MJ, Kim DH. Lactobacillus sakei OK67 ameliorates high-fat dietinduced blood glucose intolerance and obesity in mice by inhibiting gut microbiota lipopolysaccharide production and inducing colon tight junction protein expression. Nutr Res. 2016; 36 (4): 337-48.
  31. CrossRef
  32. Glantz Stanton A., Primer of Biostatistics. 4th ed., McGraw-Hill Inc., New York; 1997.
  33. Cao L, Qin X, Peterson MR, Haller SE, Wilson KA, Hu N, et al. CARD9 knockout ameliorates myocardial dysfunction associated with high fat diet-inducedobesity. J Mol Cell Cardiol. 2016; 92:185-95.
  34. CrossRef
  35. Rodríguez-Hernández H, Simental-Mendía LE, Rodríguez- Ramírez G, Reyes-Romero MA. Obesity and inflammation: epidemiology, risk factors, and markers of inflammation. Int J Endocrinol. 2013; 2013:678159.
  36. CrossRef
  37. Masters SL, Dunne A, Subramanian SL. Hull RL, Tannahill GM, Sharp FA, et al. Activation of the NLRP3 inflammasome by islet amyloid polypeptide provides a Immunol. 2010; 11 (10): 897–904.
  38. Stienstra R, Joosten LA, Koenen T, van Tits B, van Diepen JA, van den Berg SA, et al. The inflammasome-mediated caspase-1 activation controls adipocyte differentiation and insulin sensitivity. Cell Metab. 2010; 12 (6): 593–605.
  39. CrossRef
  40. Kaya MS, Bayıroglu F, Mis L, Kilinc D, Comba B. In case of obesity, longevity-related mechanisms lead to anti-inflammation. Age (Dordr). 2014; 36 (2): 677–87.
  41. CrossRef
  42. Savcheniuk OA, Virchenko OV, Falalyeyeva TM, Babenko LP, Lazarenko LM et al. The efficacy of probiotics for monosodium glutamate-induced obesity: dietology concerns and opportunities for prevention. EPMA J. 2014; 5:2.
  43. CrossRef
  44. Yang W, Cao M, Mao X, Wei X, Li X, Chen G, et al. Alternate-day fasting protects the livers of mice against high-fat diet-induced inflammation associated with the suppression of Toll-like receptor 4/nuclear factor κB signaling. Nutr Res. 2016; 36 (6): 586–93.
  45. CrossRef
  46. Vandanmagsar B, Youm YH, Ravussin A, Galgani JE, Stadler K, Mynatt RL, et al. The NLRP3 inflammasome instigates obesity-induced inflammation and insulin resistance. Nat Med. 2011; 17 (2): 179–88.
  47. CrossRef
  48. Finucane OM, Lyons CL, Murphy AM, Reynolds CM, Klinger R, Healy NP, et al. Monounsaturated fatty acid-enriched high-fat diets impede adipose NLRP3 inflammasome-mediated IL-1β secretion and insulin resistance despite obesity. Diabetes. 2015; 64 (6): 2116–28.
  49. CrossRef
  50. Lalitha V, Pal GK, Pal P, Babu MS. Neuroimmunomodulation in obesity. Internat J Clin Exp Physiol. 2015; 2(2): 97-102.
  51. CrossRef
  52. Wen H, Gris D, Lei Y, Jha S, Zhang L, Huang MT, et al. Fatty acid-induced NLRP3-ASC inflammasome activation interferes with insulin signaling. Nat Immunol. 2011; 12 (5): 408–15.
  53. CrossRef
  54. Salah Ben R, Trabelsi I, Hamden K, Chouayekh H, Bejar S. Lactobacillus plantarum TN8 exhibits protective effects on lipid, hepatic and renal profiles in obese rat. Anaerobe. 2013; 23: 55-61.
  55. CrossRef

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2024.