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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2017; 63(1): 3-9


PYRIDOXAL-5-PHOSPHATE RESTORES HYDROGEN SULFIDE SYNTHES AND REDOX STATE OF HEART AND BLOOD VESSELS TISSUE IN OLD ANIMALS

L.A. Mys, A.Yu. Budko, N.A. Strutynska, V.F. Sagach

    O.O. Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv.
DOI: https://doi.org/10.15407/fz63.01.003

Abstract

It was shown the alterations in hydrogen sulfide (H2S) metabolism and the development of oxidative and nitrozative stress in cardiovascular system by aging. The administration of pyridoxal-5-phosphate as cofactor of H2S synthesizing enzymes restored endogenous H2S level and redox state in the heart and aorta tissues. Under these conditions, the following indicators of oxidative stress were significantly decreased in heart and aorta tissues: superoxide generation rate (•О2 -) and hydroxyl (•OH) anion radicals, compared with significantly elevated levels of these parameters in old animals. We also found the reduction of non-enzymatic (diene conjugates and malonic dialdehyde) and enzymatic (uric acid, LTC4 and TxB2) lipid oxidation products levels in old rats under H2S synthesis stimulation that confirms the restriction of oxidative stress. An important consequence of endogenous synthesis stimulation of hydrogen sulfide during aging is a decrease of nitrozative stress, such as iNOS activity and nitrate reductase, as well as recovery of constitutive NO synthase activity, indicating the importance of this gas transmitter in cardiovascular system. Thus, stimulation of hydrogen sulfide endogenous synthesis contributed to reduced production of reactive oxygen species (oxidative stress) and nitrogen (nitrozative stress) in heart and aorta tissues with aging. The presence of a pronounced antioxidant effect and modulating influence of pyridoxal-5- phosphate in the redox state of heart tissue and blood vessels during aging suggests cardioprotective properties of the substance and prospects for future research. Key words: heart; aorta; hydrogen sulfide; oxidative and nitrozative stress; aging; rats.

Keywords: heart; aorta; hydrogen sulfide; oxidative and nitrozative stress; aging; rats

References

  1. Koltover VK. Free Radical Timer of Aging: from Chemistry of Free Radicals to Systems Theory of Reliability. Curr Aging Sci. 2016; 9(4).
  2. Schöttker B, Brenner H, Jansen EH, Gardiner J, Peasey A, Kubínová R, Pająk A, Topor-Madry R, Tamosiunas A, Saum KU, Holleczek B, Pikhart H, Bobak M. Evidence for the free radical/oxidative stress theory of ageing from the CHANCES consortium: a meta-analysis of individual participant data. BMC Med. 2015;13:300:1-15.
  3. CrossRef
  4. Dikalov SI, Dikalova AE. Contribution of mitochondrial oxidative stress to hypertension. Curr Opin Nephrol Hypertens. 2016;25(2):73-80. CrossRef
  5. Larousserie F, Bsiri L, Dumaine V, Dietrich C, Audebourg A, Radenen-Bussière B, Anract P, Vacher-Lavenu MC, Devergne O. Frontline Science: Human bone cells as a source of IL-27 under inflammatory conditions: role of TLRs and cytokines. J Leukoc Biol. 2016.3HI0616-280R:1-12.
  6. Ziegler DV, Wiley CD, Velarde MC. Mitochondrial effectors of cellular senescence: beyond the free radical theory of aging. Aging Cell. 2015;14(1):1-7. CrossRef
  7. Barja G. The mitochondrial free radical theory of aging. Prog Mol Biol Transl Sci. 2014;127:1-27. CrossRef
  8. Jenkins TA, Nguyen JC, Hart JL. Decreased vascular H2S production is associated with vascular oxidative stress in rats fed a high-fat western diet. Naunyn Schmiedebergs Arch Pharmacol. 2016;389(7):783-90. CrossRef
  9. Kimura H. Hydrogen polysulfide (H2Sn) signaling along with hydrogen sulfide (H2S) and nitric oxide (NO). J Neural Transm (Vienna). 2016; 123(11):1235-45. CrossRef
  10. Wang R. Physiological implication of hydrogen sulfide: a whiff exploration that blossomed. Physiol Rev. 2012; 92(2):791-896. CrossRef
  11. Kimura H. Hydrogen sulfide and polysulfides as signaling molecules. Proc Jpn Acad Ser B Phys Biol Sci. 2015; 91(4):131-59. CrossRef
  12. Strutynska NA, Semenykhina OM, Chorna SV, Vavilova GL, Sagach VF. Hydrogen sulfide Inhibits Ca2+-induced Mitochondrial Permeability Transition Pore Opening in Adultand Old Rat Heart. Fiziol Zh. 2011; 57(6):3-13. [Ukrainian].
  13. Jin S, Pu SX, Hou CL, Ma FF, Li N, Li XH, Tan B, Tao BB, Wang MJ, Zhu YC. Cardiac H2S Generation Is Reduced in Ageing Diabetic Mice. Oxid Med Cell Longev. 2015: 758358:1-14. CrossRef
  14. Drachuk K.O, Kotsjuruba A.V, Sagach V.F. Hydrogen sulfide donor, NaHS, recovers constitutive NO synthesis and endothelium-dependent relaxation of isolated aorta in old rats. Fiziol Zh. 2015; 61(6):3-10. [Ukrainian].CrossRef
  15. Zaichko N.V., Yoltukhivskiy M.M., Olhovskiy A.S., Palamarchuk I.V. Propargylglycine and Sodium Hydrogen Sulfide Age-Related Influence on Indices of Rats' Myocardium H2S Metabolism. Series problems of the biology and medicine. 2013; 4(2): 105-10. [Ukrainian].
  16. Sharipov R.R., Kotsiuruba A.V., Kop'iak B.S., Sahach V.F. Induction of nitrosative stress in mitochondria of rats hearts in experimental ischemia-reperfusion of the brain and its correction by ecdysterone. Fiziol Zh. 2014; 60(5):3-13. [Ukrainian].
  17. Sharipov R.R., Kotsiuruba A.V., Kop'iak B.S., Sahach V.F. Induction of oxidative stress in heart mitochondria in brain focal ischemia-reperfusion and protective effect of ecdysterone. Fiziol Zh. 2014; 60(3):11-7. [Ukrainian].
  18. Strutyns'kyĭ RB, Kotsiuruba AV, Rovenets' RA, Strutyns'ka NA, Iagupols'kyĭ IuL, Sagach VF, Moĭbenko OO. Biochemical mechanisms of the cardioprotective effect of the K(ATP) channels opener flocalin (medicinal form) in ischemia-reperfusion of myocardium. Fiziol Zh. 2013;59(4):16-27. [Ukrainian].
  19. Bachmann S. Mundel P. Nitric oxide in the kidney: synthesis, localization, and function. Amer J Kidney Diseases. 1994; 24:112–29. CrossRef

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