PYRIDOXAL-5-PHOSPHATE RESTORES HYDROGEN SULFIDE SYNTHES AND REDOX STATE OF HEART AND BLOOD VESSELS TISSUE IN OLD ANIMALS
L.A. Mys, A.Yu. Budko, N.A. Strutynska, V.F. Sagach
O.O. Bogomolets Institute of Physiology, National Academy
of Sciences of Ukraine, Kyiv.
It was shown the alterations in hydrogen sulfide (H2S)
metabolism and the development of oxidative and nitrozative
stress in cardiovascular system by aging. The administration
of pyridoxal-5-phosphate as cofactor of H2S synthesizing
enzymes restored endogenous H2S level and redox state in the
heart and aorta tissues. Under these conditions, the following
indicators of oxidative stress were significantly decreased in
heart and aorta tissues: superoxide generation rate (•О2
hydroxyl (•OH) anion radicals, compared with significantly
elevated levels of these parameters in old animals. We also
found the reduction of non-enzymatic (diene conjugates and
malonic dialdehyde) and enzymatic (uric acid, LTC4 and TxB2)
lipid oxidation products levels in old rats under H2S synthesis
stimulation that confirms the restriction of oxidative stress. An
important consequence of endogenous synthesis stimulation
of hydrogen sulfide during aging is a decrease of nitrozative
stress, such as iNOS activity and nitrate reductase, as well as
recovery of constitutive NO synthase activity, indicating the
importance of this gas transmitter in cardiovascular system.
Thus, stimulation of hydrogen sulfide endogenous synthesis
contributed to reduced production of reactive oxygen species
(oxidative stress) and nitrogen (nitrozative stress) in heart
and aorta tissues with aging. The presence of a pronounced
antioxidant effect and modulating influence of pyridoxal-5-
phosphate in the redox state of heart tissue and blood vessels
during aging suggests cardioprotective properties of the
substance and prospects for future research.
Key words: heart; aorta; hydrogen sulfide; oxidative and
nitrozative stress; aging; rats.
heart; aorta; hydrogen sulfide; oxidative and nitrozative stress; aging; rats
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