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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2015; 61(4): 48-55


M.S. Shypshyna1, N.S.Veselovsky1, N.F. Myasoedov2, S.I. Shram2, S.A. Fedulova1

  1. O.O. Bogomoletz Institute of Physiology National Academy of Sciences of Ukraine, Kyiv;
  2. Institute of Molecular Genetics RAS, Moscow, Russia


The influence of long-term culturing (12 days in vitro) of dorsal root ganglion (DRG) and dorsal horn (DH) neurons with peptide Semax on the level of synaptic activity at cocultures, as well as short-term plasticity in sensory synapses were studied. It has been shown that neuronal culturing with peptide at concentrations of 10 and 100 µM led to increasing the frequency of spontaneous glutamatergic postsynaptic currents in DH neurons to 71.7±1,8% and 93.9±3,1% (n=6; P<0.001). Semax has a not significant effect on the amplitude and frequency of miniature glutamatergic currents, but causes an increase of the amplitudes of spontaneous postsynaptic currents, as well as elevates the quantum content. The data show the increase of multivesicular glutamate release efficiency in neural networks of co-cultures following incubation with the peptide. Also Semax (10 and 100 µM) induces changes of the basic parameters of short-term plasticity in sensory synapses: (1) increasing the paired-pulse ratio from 0.53±0.028 (n=8) to 0.91±0.072 (n=6, P<0.01) and 0.95±0.026 (n=7; P<0.001); (2) reducing the ratio of the coefficients of variation (CV2/ CV1) from 1.49±0.11 (n=8) to 1.02±0.09 (n=6; P<0.05) and 1.11±0.13 (n=7; P<0.01) respectively. The results indicate a stimulating effect of Semax on the activity of glutamatergic synapses in neural networks of co-cultures, as well as the ability of the peptide to effectively modulate the short-term plasticity in sensory synapses.

Keywords: dorsal root ganglion; dorsal horn of the spinal cord; glutamate; postsynaptic currents; synaptic activity; short-term plasticity; peptide Semax.


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