EFFECT OF PEPTIDE SEMAX ON SYNAPTIC ACTIVITY AND SHORT-TERM PLASTICITY OF GLUTAMATERGIC SYNAPSES OF CO-CULTURED DORSAL ROOT GANGLION AND DORSAL HORN NEURONS
M.S. Shypshyna1, N.S.Veselovsky1, N.F. Myasoedov2, S.I. Shram2, S.A. Fedulova1
- O.O. Bogomoletz Institute of Physiology National Academy
of Sciences of Ukraine, Kyiv;
- Institute of Molecular Genetics RAS, Moscow, Russia
The influence of long-term culturing (12 days in vitro) of
dorsal root ganglion (DRG) and dorsal horn (DH) neurons
with peptide Semax on the level of synaptic activity at cocultures,
as well as short-term plasticity in sensory synapses
were studied. It has been shown that neuronal culturing with
peptide at concentrations of 10 and 100 µM led to increasing
the frequency of spontaneous glutamatergic postsynaptic
currents in DH neurons to 71.7±1,8% and 93.9±3,1% (n=6;
P<0.001). Semax has a not significant effect on the amplitude
and frequency of miniature glutamatergic currents, but causes
an increase of the amplitudes of spontaneous postsynaptic currents,
as well as elevates the quantum content. The data show
the increase of multivesicular glutamate release efficiency in
neural networks of co-cultures following incubation with the
peptide. Also Semax (10 and 100 µM) induces changes of the
basic parameters of short-term plasticity in sensory synapses:
(1) increasing the paired-pulse ratio from 0.53±0.028 (n=8)
to 0.91±0.072 (n=6, P<0.01) and 0.95±0.026 (n=7; P<0.001);
(2) reducing the ratio of the coefficients of variation (CV2/
CV1) from 1.49±0.11 (n=8) to 1.02±0.09 (n=6; P<0.05) and
1.11±0.13 (n=7; P<0.01) respectively. The results indicate a
stimulating effect of Semax on the activity of glutamatergic
synapses in neural networks of co-cultures, as well as the
ability of the peptide to effectively modulate the short-term
plasticity in sensory synapses.
dorsal root ganglion; dorsal horn of the spinal cord; glutamate; postsynaptic currents; synaptic activity; short-term plasticity; peptide Semax.
- Stavchansky VV, Yuzhakov VV, Botsina AY, Skvortsova VI, Bondurko LN, Tsyganova MG, Limborska SA, Myasoedov NF, Dergunova LV. The effect of Semax and its C-end peptide PGP on the morphology and proliferative activity of rat brain cells during experimental ischemia: a pilot study. J Mol Neurosci. 2011; 45(2):177-85.
- Polunin GS, Makarov IA, Shirshikov IK, Makashova NV. The efficacy of the antioxidant preparation Histochrome in the treatment of hemophthalmos in hypertension and diabetes mellitus. Vestn Oftalmol. 2000; 116(2):19-20.
- Kamkin AG, Kiseleva IS, Kositskiĭ GI. Role of ACTH fragments in regulating electrogenesis and electrotonic synaptic interaction of pond snail neurons. Fiziol Zh SSSR Im I M Sechenova. 1986; 72(7):908-20 [Russian].
- Dmitrieva VG, Povarova OV, Skvortsova VI, Limborska SA, Myasoedov NF, Dergunova LV. Semax and ProGly-Pro activate the transcription of neurotrophins and their receptor genes after cerebral ischemia. Cell Mol Neurobiol. 2010; 30(1):71-9.
- Shadrina M, Kolomin T, Agapova T, Shram S, Slominsky P, Lymborska S, Myasoedov N. Comparison of the temporary dynamics of NGF and BDNF gene expression in rat hippocampus, frontal cortex, and retina under Semax action. J Mol Neurosci. 2010; 41(1):30-5.
- Ivanova DM, Levitskaya NG, Andreeva LA, Kamenskii AA, Myasoedov NF. Comparative study of analgesic potency of ACTH4-10 fragment and its analog semax. Bull Exp Biol Med. 2007; 143(1):5-8.
- Manchenko DM, Glazova NI, Levitskaia NG, Andreeva LA, Kamenskiĭ AA, Miasoedov NF. Nootropic and analgesic effects of Semax following different routes of administration. Ross Fiziol Zh Im I M Sechenova. 2010; 96(10):1014-23 [Russian].
- Shypshyna MS, Veselovsky MS. Characteristics of sensory neurotransmission in co-culture of neurons from the dorsal root ganglion and dorsal horn spinal cord in rats. Fiziol Zh. 2010; 56(4):26-36 [Ukrainian].
- Gottmann K, Pfrieger FW, Lux HD, The formation of glutamatergic synapses in cultured central neurons: selective increase in miniature synaptic currents. Brain Res Dev Brain Res. 1994; 81(1):77-88.
- Edwards FA, Konnerth A, Sakmann B, Quantal analysis of inhibitory synaptic transmission in the dentate gyrus of rat hippocampal slices: a patch-clamp study. J. Physiol. 1990; 430:213-249.
- Shypshyna MS, Veselovsky MS. Properties of quantum release of glutamate and glycine in synapses between cocultured primary afferent and spinal dorsal horn neurons. Neurophysiology. 2013; 45(2):103-108.
- Shypshyna MS, Fedulova SA, Veselovsky NS. Induction of long-term depression of synaptic transmission in a coculture of DRG and spinal dorsal horn neurons of rats. Neurophysiology. 2011; 43(4):261-270.
- Wilcox KS, Dichter MA. Paired pulse depression in cultured hippocampal neurons is due to a presynaptic mechanism independent of GABAB autoreceptor activation. J Neurosci. 1994; 14(3): 1775-88.