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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2015; 61(4): 41-47


A.G. Reznikov, N.D. Nosenko, L.V. Tarasenko, A.A. Limareva

    State Institution “V.P. Komissarenko Institute of Endocrinology and Metabolism of National Academy of Medical Sciences of Ukraine”, Kyiv


The changes of aromatase and 5α-reductase activities were studied in preoptic area (POA) and medial basal hypothalamus of 10-days-old and sexual behavior in 3-month-old male offsprings of rats exposed daily to noradrenaline antagonist methyldopa (400 mg/kg per os) 30 minutes prior to 1-hour immobilization during the last week of pregnancy (from 15th to 21st day). Prenatal stress caused aromatase activity lowering in the POA of developing brain and feminization (appearance of lordosis) and demasculinization of sexual behavior (prolongation of latent periods to the first mounting and first intromission as well as of the first ejaculation and postejaculation refractory period) in young male offspring. Oral methyldopa used prior to pregnant females stressing prevented early effect of prenatal stress on aromatase activity in the POA and normalized the male sexual behavior in young male rats by shortening both latent period to the first ejaculation and postejaculation refractory period, and an increase of numbers of ejaculation. The data obtained indicate that brain noradrenergic system plays significant role in the mechanisms of metabolic and behavioral disturbances developing in male rats exposed to prenatal stress.

Keywords: prenatal stress; methyldopa; testosterone metabolism; sexual behavior; male rats.


  1. Reznikov AG, Pyshak VP, Nosenko ND, Tkachuk SS, Myslitsky VF. Prenatal stress and neuroendocrine pathology. Tchernovtsy:Medakademia Publishers. 2004; 320 p. [Ukrainian].
  3. Ward I. Prenatal stress feminizes and demasculinizes the behavior of males. Science.1972;175:82-4. CrossRef PubMed
  5. Rohde W, Ohkawa T, Dobashi K, Arai K, Okinaga S, Dorner G. Acute effects of maternal stress on fetal blood catecholamines and hypothalamic LH-RH content. Exp Endocr. 1983;82(3):268-74. CrossRef  
  6. Ward I, Weisz J. Maternal stress alters plasma testosterone in fetal males. Science.1980;207:328-9. CrossRef PubMed
  8. Ward IL, Weisz J. Differential effects of maternal stress on circulating levels of corticosterone, progesterone and testosterone in male and female rat fetuses and their mothers. Endocrinology. 1984;114:1635-43. CrossRef PubMed
  10. Reznikov AG, Nosenko ND, Tarasenko LV, Sinitsyn PV, Polyakova LI, Mishunina TM. Neuroendocrine effects of prenatal exposure to exogenous glucocorticoids. Russ Physiol J. 2006;92(2):238–48. [Ukrainian].
  12. Reznikov AG, Nosenko ND, Tarasenko LV. Opioids are responsible for neurochemical feminization of the brain in prenatally stressed males. Neuroendocrinol Letts. 2005;26(1): 35–8. [Ukrainian].
  14. Reznikov AG, Nosenko ND, Tarasenko LV, Sinitsyn PV, Lymareva AA. Prenatal dexamethasone prevents early and long-lasting neuroendocrine and behavioral effects of maternal stress on male offspring. Fisiol. J. 2008;54(5):28–39. [Ukrainian].
  16. Nosenko ND, Limareva AA, Reznikov AG. Preventive effect of nimodipine on early postnatal modifications of the protein spectrum in the brain of rats subjected to prenatal stress. Neurophysiology. 2012;44(1):20–5. [Ukrainian].
  18. Nosenko ND, Sinitsyn PV, Reznikov AG. Role of calcium signaling in the development of prenatal stress-induced functional modifications of the hypothalamo-pituitary-adrenal axis. Neurophysiology. 2010;42(4):251-7. [Ukrainian].
  20. MacLusky ND, Philip A, Hulburt C, Naftolin F. Estrogen formation in the developing rat brain: sex differences in aromatase activity during early postnatal life. Psychoneuroendocrinology. 1985;77(3):355–61. CrossRef  
  21. Holson RR, Gough B, Sullivan P, Badger T, Sheehan DM. Prenatal dexamethasone or stress but not ACTH or corticosterone alter sexual behavior in male rats. Neurotoxicol Teratol. 1995;17:393–401. CrossRef  
  22. Weisz J, Brown Bl, Ward Il. [Maternal stress decreases steroid aromatase activity in brains of male and female rat fetuses]. Neuroendocrinology.1982;35:374-9. CrossRef PubMed
  24. Murase J. The effect of maternal stress on aromatase activity in the perinatal rat brain. Nippon Naibunpi Gakkai Zasshi. 1994;70:95-104.
  26. Dorner G, Gotz F, Docke WD. Prevention of demasculinization and feminization of the brain in prenatally stressed male rats by perinatal androgen treatment. Exp Clin Endocrinol. 1983;81:88-90. CrossRef PubMed
  28. Rohde W, Ohkawa T, Gotz F, Stahl F, Tonjes R, Takeshita S, Arakawa S, Kambegawa A, Arai K, Okinaga S, Dorner G. Sex-specific effects on the fetal neuroendocrine system during acute stress in late pregnancy of rat and the influence of a simultaneous treatment by tyrosine. Exp Clin Endocrinol. 1989;94(1):23-42. CrossRef  
  29. Douglas AJ. Central noradrenergic mechanisms underlying acute stress responses on the hypothalamo-pituitaryadrenal axis: adaptations through pregnancy and lactation. Stress. 2005;8(1):5-19. CrossRef PubMed
  31. Rivier C, Rivest S. Effect of stress on the activity of the hypothalamic-pituitary-gonadal axis: peripheral and central mechanisms. Biol Reprod.1991;45:523-32. CrossRef PubMed
  33. Reznikov AG, Nosenko ND. It is possible that noradrenaline is the biogenic monoamine responsible for androgen-dependent sexual brain differentiation. Exp Clin Endocrinol. 1983;81:91-3. [Ukrainian].
  35. Ohkawa T, Rohde W, Gotz F, Tonjes R, Stahl F, Arai K, Okinaga S, Dorner G. The effect of an acute maternal stress on endorphin and growth hormone releasing factor in the rat fetus. Exp Clin Endocrinol. 1988;91(1):35-42. CrossRef PubMed
  37. Kashon ML, Ward OB, Grisham W, Ward IL. Prenatal ß-endorphin can modulate some aspects of sexual differentiation in rats. Behav Neurosci. 1992;106:555-62. CrossRef PubMed
  39. Ward OB, Orth JM, Weisz J. A possible role of opiates in modifying sexual differentiation. Monogr Neural Sci. 1983;9:194-200.
  41. Keshet GI, Weinstock M. Maternal naltrexone prevents morphological and behavioral alterations induced in rats by prenatal stress. Pharmac Biochem Behav.1995;50:413-9. CrossRef  
  42. Ward OB, Monaghan EP, Ward IL. Naltrexone blocks the effects of prenatal stress on sexual behavior differentiation in male rats. Pharmac Biochem Behav.1986;25:573-6. CrossRef

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