Українська English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2015; 61(4): 30-34


S.A. Rykov1, Y.Y. Byts1, S.V. Goncharov2, V.E. Dosenko2

  1. “Eye Microsurgery Center”, Kyiv;
  2. O.O. Bogomoletz Institute of Physiology National Academy of Sciences of Ukraine, Kyiv


To investigate the genetical precursors of cataract development the next groups were included: patients suffering from cataract (96) and 96 healthy persons. The determination of γ-crystallin polymorphism (G-47→A) (rs2289917) was provided using PCR method and further analyses of restriction fragment length polymorphism. These allelic variants have the significant different: G/G - 35,37 %, G/A - 53,66 %, A/A - 10,98 %, and G/G - 55,06 %, G/A - 35,96 %, A/A - 8,99 % comparing with the control group (P=0,03, by χ2-test). While investigating the level of expression of γ-crystallin gene (CRYGB) in platelets, showed that the quantity of mRNA in homozygotes G/G in 3,9 times (P<0,05) higher than in carriers of A allele (genotype G/A and A/A). This paper shows the significant difference in distribution of CRYGB promoter (G-47→A) genotypes in patients with cataract compared to the control group. Furthermore, here we provide the data concerning its functional meaning: level of mRNA expression of crystallin is different in carriers of various CRYGB promoter (G-47→A) genotypes.

Keywords: crystallin; single nucleotide polymorphism; cataract.


  1. Lin Q, Zhou N, Zhang N, Zhu B, Hu S, Zhou Z, Qi Y. Genetic variations and polymorphisms in the ezrin gene are associated with age-related cataract. Mol Vis. 2013 Jul 20;19:1572-9.
  3. Su S, Yao Y, Zhu R, Liang C, Jiang S, Hu N, Zhou J, Yang M, Xing Q, Guan H. The associations between single nucleotide polymorphisms of DNA repair genes, DNA damage, and age-related cataract: Jiangsu Eye Study. Invest Ophthalmol Vis Sci. 2013 Feb 1;54(2):1201-7.
  5. Zuercher J, Neidhardt J, Magyar I, Labs S, Moore AT, Tanner FC, Waseem N, Schorderet DF, Munier FL, Bhattacharya S, Berger W, Kloeckener-Gruissem B. Alterations of the 5'untranslated region of SLC16A12 lead to age-related cataract. Invest Ophthalmol Vis Sci. 2010 Jul;51(7):3354-61.
  7. Barreiro LB, Laval G, Quach H, Patin E, Quintana-Murci L. Natural selection has driven population differentiation in modern humans. Nat Genet. 2008 Mar;40(3):340-5.
  9. Kapur S, Mehra S, Gajjar D, Vasavada A, Kapoor M, Sharad S, Alapure B, Rajkumar S. Analysis of single nucleotide polymorphisms of CRYGA and CRYGB genes in control population of western Indian origin. Indian J Ophthalmol. 2009 May-Jun;57(3):197-201.
  11. Santhiya ST, Manisastry SM, Rawlley D, Malathi R, Anishetty S, Gopinath PM, Vijayalakshmi P, Namperumalsamy P, Adamski J, Graw J. Mutation analysis of congenital cataracts in Indian families: identification of SNPS and a new causative allele in CRYBB2 gene. Invest Ophthalmol Vis Sci. 2004 Oct;45(10):3599-607.
  13. Graw J. Genetics of crystallins: cataract and beyond. Exp Eye Res. 2009 Feb;88(2):173-89.
  15. VanderVeen DK, Andrews C, Nihalani BR, Engle EC. Crystalline cataract caused by a heterozygous missense mutation in γD-crystallin (CRYGD). Mol Vis. 2011;17:3333-8.
  17. Weisschuh N, Aisenbrey S, Wissinger B, Riess A. Identification of a novel CRYBB2 missense mutation causing congenital autosomal dominant cataract. Mol Vis. 2012;18:174-80.
  19. Mehra S, Kapur S, Vasavada AR. Polymorphisms of the gamma crystallin A and B genes among Indian patients with pediatric cataract. J Postgrad Med. 2011;57:201-5. CrossRef PubMed

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2024.