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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2015; 61(4): 30-34


ALLELIC VARIANT FREQUENCY OF PROMOTER (G-47→A) γ-CRYSTALLIN GENE AFFECTS THE LEVEL OF ITS EXPRESSION IN PLATELETS

S.A. Rykov1, Y.Y. Byts1, S.V. Goncharov2, V.E. Dosenko2

  1. “Eye Microsurgery Center”, Kyiv;
  2. O.O. Bogomoletz Institute of Physiology National Academy of Sciences of Ukraine, Kyiv
DOI: https://doi.org/10.15407/fz61.04.030

Abstract

To investigate the genetical precursors of cataract development the next groups were included: patients suffering from cataract (96) and 96 healthy persons. The determination of γ-crystallin polymorphism (G-47→A) (rs2289917) was provided using PCR method and further analyses of restriction fragment length polymorphism. These allelic variants have the significant different: G/G - 35,37 %, G/A - 53,66 %, A/A - 10,98 %, and G/G - 55,06 %, G/A - 35,96 %, A/A - 8,99 % comparing with the control group (P=0,03, by χ2-test). While investigating the level of expression of γ-crystallin gene (CRYGB) in platelets, showed that the quantity of mRNA in homozygotes G/G in 3,9 times (P<0,05) higher than in carriers of A allele (genotype G/A and A/A). This paper shows the significant difference in distribution of CRYGB promoter (G-47→A) genotypes in patients with cataract compared to the control group. Furthermore, here we provide the data concerning its functional meaning: level of mRNA expression of crystallin is different in carriers of various CRYGB promoter (G-47→A) genotypes.

Keywords: crystallin; single nucleotide polymorphism; cataract.

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