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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2015; 61(1): 83-90


MODIFICATION OF EXPERIMENTAL ROTENONE MODEL OF PARKINSON’S DISEASE

L.P. Voitenko, A.G. Nikonenko

    O.O.Bogomoletz Institute of Physiology of National Academy of Sciences of Ukraine, Kyiv
DOI: https://doi.org/10.15407/fz61.01.083

Abstract

A modification of experimental model of Parkinson’s disease is proposed presuming the stereotaxic infusion of rotenone solution using a special device into the cental part of substantia nigra (SN) pars compacta of adult Wistar rats. It was shown that 10 days after infusion of the neurotoxin the density of dopaminergic (DA) neurons in the infusion area drops nearly six-fold, to 20.2±3.2 neurons/mm2, with respect to the corresponding value in non-affected controlateral SN, 119.0±3.3 neurons/mm2. Electron microscopy has shown ultrastructural impairments in mitochondria of SN neurons in the infusion area displayed mainly as a cristae disarray. The absence of overall toxicity and selectivity of the brain tissue impairments provide an evidence that the proposed rotenone model modification is adequate and can be used to study the effects of DA neuronal degeneration typical of Parkinson’s disease.

Keywords: rotenone; in vivo model; Parkinson’s disease.

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