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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2015; 61(1): 56-62


EFFECT OF REDUCED OXYGEN CONCENTRATIONS AND HYDROGEN SULFIDE ON THE AMINO ACID METABOLISM AND MESENCHYMAL CELLS PROLIFERATION

L.N. Plotnikova1, V.A. Berezovskiі1, S.P. Veselskiі2

  1. O.O. Bogomolets Institute of Physiology NAS of Ukraine, Kyiv;
  2. Peter Bogach Institute of Physiology ESC «Institute of biology» T.Shevchenko Kyiv National University
DOI: https://doi.org/10.15407/fz61.01.056

Abstract

We investigated the effect of hydrogen sulfide donor (10-12 mol/l NaHS – I group) alone and together with the reduced oxygen concentrations (5% O2 – II group, 3% O2 – III group, 24 h) on the biological processes of human stem cells culture. It was shown that the cells proliferation by the third day of cultivation in I, II and III group decreased 1,7; 2,8 and 4,2 times. On the 4th day of culture proliferation inhibited in I, II and III group by 29; 33 and 54% compared to the control. Thus, adverse effects NaHS enhanced by reducing the oxygen concentration. It was established that in all experimental versions rapidly absorbed from the culture medium amino acids: cysteine and cystine, serine and aspartic acid, valine and tryptophan, proline and hydroxyproline, which are involved in the synthesis of proteins, in particular collagen. In the culture medium increased the concentration of free amino acids of the three factions: arginine, histidine and taurine; glycine and methionine; alanine and glutamine. We believe that in the applied concentration of hydrogen sulfide donor in conditions of low oxygen in a gaseous medium incubation inhibits the proliferation and alters the amino acid metabolism of human cells line 4BL.

Keywords: mesenchymal stem cells; hydrogen sulphide; amino acid composition; proliferation.

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