EFFECT OF REDUCED OXYGEN CONCENTRATIONS AND HYDROGEN SULFIDE ON THE AMINO ACID METABOLISM AND MESENCHYMAL CELLS PROLIFERATION
L.N. Plotnikova1, V.A. Berezovskiі1, S.P. Veselskiі2
- O.O. Bogomolets Institute of Physiology NAS of Ukraine,
Kyiv;
- Peter Bogach Institute of Physiology ESC «Institute of
biology» T.Shevchenko Kyiv National University
DOI: https://doi.org/10.15407/fz61.01.056
Abstract
We investigated the effect of hydrogen sulfide donor (10-12
mol/l NaHS – I group) alone and together with the reduced
oxygen concentrations (5% O2 – II group, 3% O2 – III group,
24 h) on the biological processes of human stem cells culture.
It was shown that the cells proliferation by the third day of
cultivation in I, II and III group decreased 1,7; 2,8 and 4,2
times. On the 4th day of culture proliferation inhibited in I,
II and III group by 29; 33 and 54% compared to the control.
Thus, adverse effects NaHS enhanced by reducing the oxygen
concentration. It was established that in all experimental
versions rapidly absorbed from the culture medium amino
acids: cysteine and cystine, serine and aspartic acid, valine and
tryptophan, proline and hydroxyproline, which are involved in
the synthesis of proteins, in particular collagen. In the culture
medium increased the concentration of free amino acids of
the three factions: arginine, histidine and taurine; glycine and
methionine; alanine and glutamine. We believe that in the
applied concentration of hydrogen sulfide donor in conditions
of low oxygen in a gaseous medium incubation inhibits the
proliferation and alters the amino acid metabolism of human
cells line 4BL.
Keywords:
mesenchymal stem cells; hydrogen sulphide; amino acid composition; proliferation.
References
- Lisianyi NI. Mesenchymal stem cells and immunological properties. Fiziol Zh. 2013;59(3):126-34.
- Margini C, Vukotic R, Brodosi L, Bernardi M, Andreone P. Bone marrow derived stem cells for the treatment of end-stage liver disease. World J Gastroenterol. 2014;20(27):9098-105.
- Wang X, Zhang H, Nie L, Xu L, Chen M, Ding Z. Myogenic differentiation and reparative activity of stromal cells derived from pericardial adipose in comparison to subcutaneous origin. Stem Cell Res Ther. 2014;5(4):92.
CrossRef
PubMed PubMedCentral
- Buravkova LB, Grinakovskaya OS, Andreeva ER, Zhambalova AP, Kozionova MP. Characteristics of human lipoaspirate-isolated mesenchymal stromal cells cultivated under a lower oxygen tension. Sytology. 2009;51(1):5-11.
CrossRef
- Rylova YuV, Buravkova LB. Long-term expansion of multipotent mesenchymal stromal cells under reduced oxygen tension. Cytology. 2013;55(12):852-60.
- Lavrentieva A, Majore I, Kasper C, Hass R. Effects of hypoxic culture conditions on umbilical cord-derived human mesenchymal stem cels. Cell Commun Signal. 2010;8:18-27.
CrossRef
PubMed PubMedCentral
- Liu D, Wang Z, Zhan J, Zhang Q, Wang J, Zhang Q, Xian X, Luan Q, Hao A. Hydrogen sulfide promotes proliferation and neuronal differentiation of neural stem cells and protects hypoxia-induced decrease in hippocampal neurogenesis. Pharmacol Biochem Behav. 2014;116:55-63.
CrossRef
PubMed
- Sheng J, Shim W, Wei H, Lim S, Liew R, Lim T, Ong B, Chua Y, Wong Ph. Hydrogen sulfide suppresses human atrial fibroblast proliferation and transformation to myofibroblasts. J Cell Mol Med. 2013;17(10):1345-54.
CrossRef
PubMed PubMedCentral
- Lukash L, Yatsishina A, Kushniruk V, Pidpala O. Reprogramming of somatic cells in the adult human in vitro. Factors Exp Evol Organisms. 2011;11:493-8.
- Adams R. Methods of cell culture for biochemists. Moscow: Mir. 1983:264p.
- Kaznacheeva A, Zlydnev N. The content of free amino acids in healthy blood plasma, erythrocytes and urine. Lab Work. 1976;8:479-80.
- Korobeinikova E, Meshcheriakova G. Determination of free amino acids in the serum and urine of healthy children. Lab Work. 1981;4:221-4.
- Kimura H. Hydrogen sulfide: its production, release and functions. Amino acids. 2010;45:56-61.
- Kimura Y, Kimura H. Hydrogen sulfide protects neurons from oxidative stress. FASEB J. 2004;18(10):1165-7.
CrossRef
- Strutynska N, Semenykhina S, Vavilova G, Sagach V. Hydrogen sulfide inhibits Ca2+-induced mitochondrial permeability transition pore opening in adult and old rat heart. Fiziol Zh. 2011;57(6):3-14.
- Liu Y, Yang R, Liu X, Zhou Y, Qu C, Kikuiri T, Wang S, Zandi E, Du J, Ambudkar IS, Shi S. Hydrogen sulfide maintains mesenchymal stem cell function and bone homeostasis via regulation of Ca(2+) channel sulfhydration. Cell Stem Cell. 2014;15(1):66-78.
CrossRef
PubMed PubMedCentral
- Okada M, Ishkitiev N, Yaegaki K, Imai1 T, Tanaka T, Fukuda M, Ono S, Haapasalo M. Hydrogen sulphide increases hepatic differentiation of human tooth pulp stem cells compared with human bone marrow stem cells. Article first published online: 20 MAR 2014. DOI: 10.1111/iej.12262.
CrossRef
- Chen G, Wang J. Threonine metabolism and embryonic stem cell self-renewal. Current Opinion in Clinical Nutrition & Metabolic Care. 2014;17(1):80-5.
- Aniskina A, Chalisova N, Zakutskiy A, Komashnya A, Philippov S, Zezyulin P. Effect of amino acids on cell proliferation and apoptosis in organotypic tissue culture of rats of various age. Adv Gerontol. 2006;19:55-9.
- Gaspar JA, Doss MX, Hengstler JG, Cadenas C, Hescheler J, Sachinidis A. Unique metabolic features of stem cells, cardiomyocytes, and their progenitors. Circulation Research. 2014;114:1346-60.
CrossRef
PubMed
- Guoyao WU, Sidney M. Arginine metabolism: nitric oxide and beyond. Biochem. J. 1998;336:1-17.
- Higuera GA, Schop D, Spitters TW, van DijkhuizenRadersma R, Bracke M, de Bruijn JD, Martens D, Karperien M, van Boxtel A, van Blitterswijk CA. Patterns of amino acid metabolism by proliferating human mesenchymal stem cells. Tissue Eng Part A. 2012;18(5-6):654-64.
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