Українська English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2015; 61(1): 42-49

ROLE OF CYCLOOXYGENASE IN MODIFICATION OF INTESTINAL MICROFLORA UNDER STRESS CONDITION

I.S. Fomenko, O.P. Korniychuk, A.R. Hural’, R.G. Shykula, I.I. Ilkiv, A.Ya. Sklyarov

    D. Halytskyi National Medical University, Lviv, Ukraine
DOI: https://doi.org/10.15407/fz61.01.042


Abstract

Stress and nonsteroidal anti-inflammatory drugs, which act as nonselective inhibitors of cyclooxygenase, are the main factors of ulcerogenesis in digestive system. However, the peculiarities of their combined action upon the status of intestinal microflora and the parameters of NO-synthase system are still poorly understood. In experiments with rats we show that water-restrained stress was accompanied by a considerable increase of iNOS activity and intensity of lipoperoxidation processes. The increase of Escherichia coli content and the decrease in Enterococcus spp. concentration in the small intestine with their simultaneous rise in the large intestine were noticed under these conditions. Cyclooxygenese blockage with naproxen prior to induction of water-restrained stress was accompanied by the decease of iNOS in small and large intestines, with the synchronous rise of cNOS activity in the large intestine as compared with indexes in stress. The moderate increase in Enterococcus spp. content in duodenum with the rise of Escherichia coli concentration in the ileum was shown. The Escherichia coli content decreased in the proximal part of the large intestine and decreased in its distal part. Disbiosis, intensification of lipoperoxidation processes and changes in NO-synthase system parameters under condition of simultaneous action of stress and cyclooxygenase blockage can create preconditions for the development of destructive changes and enteropathias.

Keywords: stress; NSAIDs; nitric oxide; microflora; small intestine; large intestine.

Full text: (PDF, in Ukrainian)

References

  1. Konturek PC, Brzozowski T, Konturek SJ. Stress and the gut: phathophysiology, clinical consequences, diagnostic approach and treatment options. J Physiol Pharmacol. 2011. 62(6): 591-599.
    2.  
  2. Collins SM, Bercik P. The Relationship Between Intestinal Microbiota and the Central Nervous System in Normal Gastrointestinal Function and Disease. Gastroenterology. 2009. 136 (6): 2003–2014.
    3.  
  3. Lim YJ, Chun HJ. Recent advances in NSAIDs-induced enteropathy therapeutics: new options, new challenges. Gastroenterol Resd Pract. 2013. 2013: 1-7.
    4.  
  4. Watanabe T. Higuchi K, Kobata A, Nishio H, Tanigawa T, Shiba M, Tominaga K, Fujiwara Y, Oshitani N, Asahara T, Nomoto K, Takeuchi K, Arakawa T. Non-steroidal anti-inflammatory drug-induced small intestinal damage is Toll-like receptor 4 dependent. Gut. 2008. 57 (2): 181-187.
    5.  
  5. Uejima M, Kinouchi T, Kataoka K, Hiraoka I, Ohnishi Y. Role of intestinal bacteria in ileal ulcer formation in rats treated with a nonsteroidal antiinflammatory drug. Microbiol Immunol. 1996. 40 (8): 553-560.
    6.  
  6. Vento P, Kiviluoto T, Jarvinen HJ Soinila S. Scand J Gastroenterol. 2001. 36 (2): 180–189.
    7.  
  7. Lundberg JO, Weitzberg E. Biology of nitrogen oxides in the gastrointestinal tract. Gut. 2013. 62(4): 616-626.
    8.  
  8. Mollace V, Muscoli C, Masini E, Cuzzocrea S, Salvemini D. Modulation of prostaglandin biosynthesis by nitric oxide and nitric oxide donors. Pharmacol Res. 2005. 57 (2): 217–252.
    9.  
  9. Sklyarov AYa, Panasyuk NB, Fomenko IS. Role of nitric oxide-synthase and cyclooxygenase/lipooxygenase systems in development of experimental ulcerative colitis. J Physiol Pharmacol. 2011. 62 (1): 65-73.
    10.  
  10. Takagi KY, Kayuya Y, Watanabe K. Studies on drugs for peptic ulcer. A reliable method for producing stress ulcers in rats. Chem Pharm Bull. 1964. 12: 465-472.
    11.  
  11. Klymnjuk SI, Sytnyk IO, Tvorko MS, Shyrobokov VP. Practical microbiology. Ternopil: Ukrmedknyga; 2004: 438 p.
    12.  
  12. Sumbajev VV, Jasinskaja IM. The influence of DDT on the activity of nitric oxide synthase in liver, lungs and brain of rats. Modern problems of toxicology. 2000. 3: 3-7.
    13.  
  13. Green LC, Wagner DA, Glogowski J, Skipper PL, Wishnok JS, Tannenbaum SR. Analysis of nitrate, nitrite, and [15N] nitrate in biological fluids. Anal Biochem. 1982. 126(1): 131-138.
    14.  
  14. Geyer JW, Dabich D. Rapid method for determination of arginase activity in tissue homogenates. Anal Biochem. 1971. 39(2): 412-417.
    15.  
  15. Timirbulatov RA, Seleznev EI. Method for increasing the intensity of free radical oxidation of lipid-containing components of the blood and its diagnostic significance. Lab Delo. 1981. 4: 209-117.
    16.  
  16. Amanov AN. Quantitative relationships of distinct types of gastrointestinal microflora of experimental animals (rats) in norm and under condition of imuran immunosupression. Zh Mikrobiol Epidemiol Immunobiol. 1983; 6: 77-81.
    17.  
  17. Larauche M, Kiank C, Tache Y. Corticotropin releasing factor signaling in colon and ileum: regulation by stress and pathophysiologycal implications. J Physiol Pharmacol. 2009. 60 (7): 33-46.
    18.  
  18. Basso N, Materia A, Forlini A, Jaffe BM. Prostaglandin generation in the gastric mucosa of rats with stress ulcer. Surgery. 1983. 94 (1): 104-108.
    19.  
  19. Galley JD, Bailey MT. Impact of stressor exposure on the interplay between commensal microbiota and host inflammation. Gut Microbes. 2014. 5(3): 390-396.
    20.  
  20. Shamro NR, Panasyuk NB, Fomenko IS, Sklyarov O Ya. Influence of vitamin C on mechanisms of cytoprotection, lipid peroxidation and NO-synthases under stress and experimental colitis in rats. Biology and medicine Issues Review. 2011. 3 (86): 159-163.
    21.  
  21. Jorge E, Vergata P, Martin MT. Ileal inducible nitric oxide synthase mRNA expression in response to stress modified in Sprague-Dawley rats exposed to a previous intestinal inflammation. Stress. 2012. 15(1): 62-73.
    22.  
  22. Fomenko IS, Bondarchuk TI, Biletska LP, Panasyuk NB, Sklyarov AYa. Peculiarities of influence of nonsteroidal anti-inflammatory drugs on parameters NO-synthase system in gastric mucosa of rats under stress conditions. Fiziol Zh. 2014; 60(2): 51-56.
    23.  
  23. Ling JJ, Sun YJ, Zhu DY, Chen Q, Han X. Potential role of NO in modulation of COX-2 expression and PGE2 production in pancreatic beta-cells. Acta Biochim Biophys Sin (Shanghai). 37 (2): 139-146.
    24.  
  24. Wallace JL. NSAID gastropathy and enteropathy: distinct pathogenesis likely necessitates distinct prevention strategies. Br J Pharmacol. 2012. 165 (1): 67-74.
    25.  

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2024.