ROLE OF CYCLOOXYGENASE IN MODIFICATION OF INTESTINAL MICROFLORA UNDER STRESS CONDITION
I.S. Fomenko, O.P. Korniychuk, A.R. Hural’, R.G. Shykula, I.I. Ilkiv, A.Ya. Sklyarov
D. Halytskyi National Medical University, Lviv, Ukraine
Stress and nonsteroidal anti-inflammatory drugs, which act
as nonselective inhibitors of cyclooxygenase, are the main
factors of ulcerogenesis in digestive system. However, the
peculiarities of their combined action upon the status of
intestinal microflora and the parameters of NO-synthase
system are still poorly understood. In experiments with rats
we show that water-restrained stress was accompanied by
a considerable increase of iNOS activity and intensity of
lipoperoxidation processes. The increase of Escherichia
coli content and the decrease in Enterococcus spp. concentration
in the small intestine with their simultaneous
rise in the large intestine were noticed under these conditions.
Cyclooxygenese blockage with naproxen prior to
induction of water-restrained stress was accompanied by
the decease of iNOS in small and large intestines, with the
synchronous rise of cNOS activity in the large intestine as
compared with indexes in stress. The moderate increase
in Enterococcus spp. content in duodenum with the rise
of Escherichia coli concentration in the ileum was shown.
The Escherichia coli content decreased in the proximal
part of the large intestine and decreased in its distal part.
Disbiosis, intensification of lipoperoxidation processes and
changes in NO-synthase system parameters under condition
of simultaneous action of stress and cyclooxygenase
blockage can create preconditions for the development of
destructive changes and enteropathias.
stress; NSAIDs; nitric oxide; microflora; small intestine; large intestine.
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