THE PECULIARITIES OF ARGINASE PATHWAY OF L-ARGININE IN SPERMATOZOA IN MEN WITH DIFFERENT FORMS OF PATHOSPERMIA
R.V. Fafula, O.K. Onufrovych, U.P. Iefremova, D.Z. Vorobets, Z.D. Vorobets
Danylo Halytsky Lviv National Medical University.
The changes in arginase activity of spermatozoa and hormonal
profile of peripheral blood of infertile men with various
forms pathospermia have been studied. It has been found that
arginase activity in the sperm cells of men with oligozoo-, antenozoo-,
oligoastenozoo- and leucocytospermia is decreased
in 2.1, 2.3, 2.4 and 3.3 times respectively. This indicates about
inhibition of arginase pathway of L-arginine metabolism,
which is not significantly dependent on the type of disruption
of spermatogenesis. The most significant changes have been
observed in infertile men with leucocytospermia since white
blood cells stimulate the formation of reactive oxygen species,
induction and development of oxidative and nitrative stress
in spermatozoa. Inhibition of arginase pathway of L-arginine
metabolism has adaptive role, which is to limit bioavailability
of L-arginine and to prevent excessive formation of NO
in cytotoxic concentrations to sperm cells. It has been noted
changes in serum concentrations of gonadotropin and sex
hormones in men with various forms of pathospermia. The
most expressed significant changes were in levels of follicle
stimulating hormone and testosterone. The concentration of
follicle stimulating hormone in patients with oligozoospermia
caused by hypogonadism is twice higher and in patients with
leucocytospermia in 1.8 times higher than in fertile men. In
patients with astenozoospermia this value is in 2.2 times lower
than in normozoospermic samples but within the physiological
norm. The testosterone level in men with oligozoospermia is in
1.6 times lower than in fertile men but within the physiological
norm. It has been found that arginase inhibition of spermatozoa
positively correlated with a decrease in their concentration in
the ejaculate of infertile men with oligozoospermia (r = 0.68).
arginase; nitric oxide; infertility in men.
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