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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2016; 62(5): 76-82


I.O. Belkina1, N.P. Smolenko1, V.K. Klochkov2, Yu.V. Malukin2, Е.E. Chistyakova1, N.A. Karpenko1, Yu.I. Karachentsev1

  1. SI «V. Danilevsky Institute for Endocrine Pathology Problems of NAMS of Ukraine», Kharkov;
  2. Institute for Scintillation Materials NAS of Ukraine, Kharkov


For the purpose to develop of method for treatment of male hypofertility the efficiency of gadolinium orthovanadate nanoparticles (NP GdVO4) in rats with neonatal induced reproductive disease, has been investigated. The progeny of intact rats parents were exposed to emotional stress («Maternal separation stress») and received an excessive amount of the blend of phytoestrogens (Ph) with mother’s milk (1st group) from 3 to 22 day life. At the age of 10 months males received NP GdVO4 (0.33 mg/kg; 2nd group) or speman (158 mg/kg; 3rd group) with feed over the 70 days. Sexual behavior and fertility of males were investigated; the integrated indicator of reproductive potential (Fì) was calculate and was estimated with the data of animals from group Control who were given vehicle (water). In the 1st group sexual behavior of males was characterized by accelerating sexual responses on female (double shortening of mounting and intromission latency) and a reduced ability to achieve ejaculation and to start the second round of copulations. The less females have been inseminated by these males (67% vs. 91% in Control group), but almost all became pregnant. As a result of the neonatal induction deterioration in the quality of male gametes intrauterine pregnancy loss increased more than twice, and the total number of foetus decreased (on 21%). These changes have led to a reduction of the male integrated indicator of reproductive potential (Fì) in 2.6 times compared with control animals. Using a NP GdVO4 rehabilitated male sexual behaviour indicators to Control group level, resulting in an increase in the number of fertilized females (up to 95%). In pregnant females decreased fetal losses (up to 15.2% vs. 18.1% in control, р<0,05) and increase in the number of fetuses (on 25%, р<0,05) that shows an improvement in the quality of sperm. The integrated indicator of reproductive potential (Fì) increased to (8.3 ± 0.5) vs. (3.0 ± 0.3) u. in the 1st group (without treatment), that statistically higher than in groups Control and 3rd. Application of NP GdVO4 in rats with neonatal inductive pathology of the reproductive function and metabolic disorders normalizes sexual behaviour, the quality of sperm that restores male fertility and improves reproductive potential much more efficiently than the reference drug speman.

Keywords: neonatal stress, phytoestrogens, rear-earth-based nanoparticles, male rats, reproductive potential.


  1. Gorpynchenko I, Nikitin O. Marriages infertility. New reality. Man Health. 2010; (3):184-90. [Russian].
  3. Yatskiv O, Tarnovska A. Causes and forms of male infertility and methods of diagnosis ejaculate, as the main indicator of male health. Visn Lviv Univ Ser Biol. 2012; 60:4-20. [Ukrainian].
  5. Social Medicine and Health Organization. Voronenko YuV, Moskalenko VF, editor. Ternopil: Ukrmedkniga: 2000. [Ukrainian].
  7. The health status of Ukraine's population and the results of the health. Kiev: 2003. [Ukrainian].
  9. Eberhardt P. Distribution and dynamics of rural population in Central Eastern Europe in the 20th century. Geogr Pol. 1994. (63):75-94.
  11. Tiktinskiy OL, Mihaylichenko VV, Andrology. SanktPeterburg: Mediapress: 1999. [Russian].
  13. Kulakov VI. Infertile marriage. Current approaches to diagnosis and treatment. Eds VI Kulakov. Moskow: GAOTAP-Media: 2005. [Russian].
  15. Baccetti B, LaMarca A, Piomboni P, et al. Insulin-dependent diabetes in men is associated with hypothalamo-pituitary derangement and with impairment in semen quality. Hum Reprod. 2002;17:2673-7. CrossRef PubMed
  17. Shrivastav P, Swann J, Jeremy JY, Thompson C, Shaw RW, Dandona P. Sperm function and structure and seminal plasma prostanoid concentrations in men with IDDM. Diabetes Care. 1989;12:742-4. CrossRef PubMed
  19. Schoeller EL, Schon S, Moley KHM. The effects of type 1 diabetes on the hypothalamic, pituitary and testes axis. Cell Tissue Res. 2012;349(3):839-47. CrossRef PubMed PubMedCentral
  21. Lampiao F, Du Plessis SS. Insulin and leptin enhance human sperm motility, acrosome reaction and nitric oxide production. Asian J Androl. 2008;10:799-07. CrossRef PubMed
  23. Luchitskij YeV, Tronko ND. Androdgen deficiency states in men with metabolic syndrome and type 2 diabetes mellitus: pathogenetic role and the possibility of correcting. In: Luchitskij YeV, Bondarenko VA, editor. Selected lectures on clinical andrology. Kiev, Harkiv: Nova Soft: 2010. p. 115-43. [Ukrainian].
  25. Domingo JL. Vanadium and tungsten derivatives as antidiabetic agents: a review of their toxic effects. Biol Trace Elem Res. 2002;88(2):97-112. CrossRef  
  26. Morgan AM, El-Tawil OS. Effects of ammonium metavanadate on fertility and reproductive performance of adult male and female rats. Pharmacol Res. 2003; 47(1):75-85. CrossRef  
  27. Crebelli R, Leopardi P. Long-term risks of metal contaminants in drinking water: a critical appraisal of guideline values for arsenic and vanadium. Ann Ist Super Sanita. 2012;48(4):354-61. CrossRef PubMed
  29. Karpenko NA, Malukin YV, Koreneva EM, Klochkov VK, Kavok NS, Smolenko NP, Pochernyaeva SS. The Effects of Chronic Intake of Cerium Dioxide or Gadolinium Ortovanadate Nanoparticles in Aging Male Rats: 3-rd Proceedings of the international conference nanomaterials «Applications and properties»; 2013; Sept. 16-21; Alushta the Crimea, Ukraine. p. 04NABM28-1 – 04NABM28-4.
  31. Reznikov AG. General ethical principles of animal experimentation. Endocrinology. 2003; 8(1):142-5. [Ukrainian].
  33. Karpenko N, Chistyakova E, Korenyeva Ye. Velichko N. Method simulation induced neonatal males hipofertylnosti. Ukraine patent US 95758. 2015.
  35. Kuzmenko VV, Alferov KV. Our experience with Speman drug: Proceedings of Scientific Conference «Men's Health»; 2003; Novem. 19-21; Moscow. p. 16. [Russian].
  37. Ribolovlyev YuR. Prediction of xenobiotics on human action. Pharmacol Toxicol. 1991; (1): P. 110-4. [Russian].
  39. Bishovets TF. Experimental study embryotoxical action of drugs guidance: methodical recommendations. In: OV Stefanov, editor. Preclinical studies of drugs. Kiev: Avitsena: 2001. p. 115-38. [Ukrainian].
  41. Karpenko NO, Talko VV, Omelchuk ST, Lapta SS. Integral assessment of the reproductive function of male laboratory animals. Ukr Biopharm Zh. 2011; 13(2): 64-8. [Ukrainian].
  43. Zapadnyuk IP. Laboratory animals. Breeding, maintenance, use in experiments. Kiev: Vischa Skola: 1983. [Russian].
  45. Ingel FN, Gevorkyan NM, Ilyushina NA,. Long psychoemotional stress as an inducer of mutations in mammals and modifier mutagenesis. Bull Experim Biol Med. 1993; 116 (9):307-9. [Russian].
  47. Seli E, Gardner DK, Schoolcraft WB. Extent of nuclear DNA damage in ejaculated spermatozoa impacts on blastocyst development after in vitro fertilization. Fertil Steril. 2004; (82):378-83.
  49. Karpenko NO, Smolenko NP, Korenyeva YeM, Chistyakova EYe, Velichko NF. Dynamics and spoluchenist age involution of some components of male reproductive function. Probl Endocrin Patol. 2014; (1):49-54. [Ukrainian].

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