Українська English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2016; 62(5): 69-75

STATUS OF GLUTATHIONE-DEPENDENT LINK OF BLOOD ANTIOXIDANT SYSTEM UNDER APPLICATION OF FENUGREEK SEED POWDER IN ANIMALS WITH EXPERIMENTAL OBESITY

V.V. Konopelnyuk, L.І. Коt, Т.V. Koval, T.D. Khilko, I.V. Yakubtsova, T.D. Preobrazhenska, L.I. Ostapchenko

    Taras Shevchenko National University of Kyiv
DOI: https://doi.org/10.15407/fz62.05.069


Abstract

The content of reduced glutathione (GSH) and activity of glutathione-dependent enzymes: glutathione peroxidase (GP) and glutathione-S-transferase (GT) in blood serum of rats in the diet supplemented of fine powder seeds Trigonella foenum graecum L. on the background of experimental obesity. It was established that the development of obesity in rats was accompanied by violation of homeostasis in the glutathionedependent system of blood serum. It has been shown that animals on high-calorie diet along 14 weeks had a decrease (1,2) in GSH concentration, a decline (1,7) in GP activity and the increase (1,7) of GT activity compared with control values. Addition to high-calorie diet 2% fine powder seeds Trigonella foenum graecum L. reduced the body weight gain by 21%, carried positive modulating effect on the content of GSH and GP, GT activity in animals with experimental obesity.

Keywords: diet-induced obesity; reduced glutathione; glutathione peroxidase; glutathione transferase; fine powder seeds Trigonella foenum graecum L.

Full text: (PDF, in Ukrainian)

References

  1. Caballero B. The global epidemic of obesity: an overview. Epidemiol Rev. 2007; 29: 1-5. CrossRef PubMed
    2.  
  2. Markhon NA, Mamchur VI, Zhylyuk VI, Lievykh AE. Comparative analysis of experimental approaches in reproducing of metabolic syndrome. J Med Biol Problems. 2015; 1(17): 156-62. [Ukrainian].
    3.  
  3. Pasiyeshvili LM, Zheleznyakova NM, Pasiyeshvili TM. Obesity as a social problem. Stages of the disease formation in subjects with biliary diseases. Contemp Gastroenterol. 2008; 6: 6-9.
    4.  
  4. Patil S, Jain G. Holistic approach of Trigonella foenumgraecum in Phytochemistry and Pharmacology. Curr Trends Technol Sci. 2014; 3: 34-48.
    5.  
  5. Moradi Kor N.,•Moradi K. Physiological and pharmaceutical effects of fenugreek (Trigonella foenum-graecum L.) as a multipurpose and valuable medicinal plant. Global J Med Plant Res. 2013; 1(2): 199-206.
    6.  
  6. Kaur J, Singh H, Khan MU. Multifarious therapeutic potential of fenugreek: a comprehensive review. Int J Res Pharm Biomed. Sci. 2011; 2(3): 863-71.
    7.  
  7. Fernández-Sánchez A, Madrigal-Santillán E, Bautista M et al. Inflammation, Oxidative Stress, and Obesity. Int J Mol Sci. 2011; 12: 3117-32. CrossRef PubMed PubMedCentral
    8.  
  8. Kalinina EV, Chernov NN, Novichkova MD. Role of glutathione, glutathione transferase, and glutaredoxin in regulation of redox-dependent processes. Biochemistry. 2014; 79(13): 1562-83. CrossRef  
  9. Shen X.-H, Tang Q.-Y, Huang J, Cai W. Vitamin E regulates adipocytokine expression in a rat model of dietary-induced obesity. Experim. Biol Med. 2010; 235: 47-51.
    10.  
  10. Mokrasch LC, Teschke EJ. Glutathione content of cultured cells and rodent brain regions: a specific fluorometric assay. Anal Biochem. 1984; 140(2): 506-9. CrossRef  
  11. Hissin PJ, Hilf RA. Fluorometric method for determination of oxidized and reduced glutathione in tissues. Anal Biochem. 1976; 74(1): 214-26. CrossRef  
  12. Razygraev AV. Method of determination of glutathione peroxidase activity using hydrogen peroxide and 5,5′-dithiobis(2-nitrobenzoic acid). Klin-Lab Konsilium. 2004; 4: 19-22. [Russian].
    13.  
  13. Vlasova SN, Shabunina EI, Pereslyagina IA. Activity of glutathione dependent enzymes in red blood cells liver chronic disease in children. Lab Delo. 1990; 8: 19-21. [Russian].
    14.  
  14. Ghorbani A, Hadjzadeh M, Rajaei Z, Zendehbad SB. Effects of fenugreek seeds on adipogenesis and lipolysis in normal and diabetic rats. Pakistan J Biol Sci. 2014; 17: 523-8. CrossRef  
  15. Choudhary D; Chandra D; Choudhary S; Kale R.K. Modulation of glyoxalase, glutathione S-transferase and antioxidant enzymes in the liver, spleen and erythrocytes of mice by dietary administration of fenugreek seeds. Food Chem. 2001; 39(10): 989-97. CrossRef  
  16. Reznikov OH, Polumbryk OM, Balion YH, Polumbryk MO. Pro- and antioxidant systems and pathological processes in humans. Bull NAS Ukraine. 2014; 10: 17- 29. [Ukrainian].
    17.  
  17. Ballatori N, Krance SM, Notenboom S et al. Glutathione dysregulation and the etiology and progression of human diseases. Biol Chem. 2009; 390(3): 191-214. CrossRef PubMed PubMedCentral
    18.  
  18. Iskra R. Condition of glutathione link of antioxidant system in different organs and tissues of rats at chromium nanoaquarcitate action. Eksperim Clіn Physiol Bіochem. 2011; 3: 28-33. [Ukrainian].
    19.  
  19. Denisenko YK, Novgorodtseva TP. Effect of prolonged high-fat diet on thiol-disulfide homeostasis in rats. Int J BioMed. 2013; 3(3): 197-200.
© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2025.