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Fiziologichnyi Zhurnal - <a id="lj1_ret_bk_to_jl0_href_id" href="?list0=18">Fiziol. Zh. 2016;</a> <a id="lj1_ret_bk_to_jl0_href_id" href="?list1=96">62(5):</a> 50-56
Українська Русский English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2016; 62(5): 50-56


Flavonoid quercetin reduces gliosis after repetitive mild traumatic brain injury in mice

Y.Y. Zabenko, T.A. Pivneva

    Bogomoletz Institute of Physiology of the National Academy of Sciences of Ukraine, Kyiv
DOI: https://doi.org/10.15407/fz62.05.050

Abstract

The effect of water-soluble form of quercetin on the structural changes of glial cells in hippocampus was investigated in mice after repetitive mild traumatic brain injury. Reactive astro- and microgliosis in hippocampus were observed after brain injury. Iba-1 and GFAP immunohistochemistry was used to visualize astrocytes and microglia cells. Immunopositive cells were counted in hippocampal CA1-area at 5th, 10th and 30th days since the first injury and at 5th, 10th and 30th days since the first quercetin injection. Administration of quercetin leaded to the decrease in number of activated glial cells. Thus, our study demonstrates the following: repetitive mild traumatic brain injury in mice is associated with reactive gliosis; quercetin showed neuroprotective effects by reducing this gliosis. In view of the described, use of quercetin is appropriate for pharmacological correction of cerebrovascular disorders after traumatic brain injury.

Keywords: repetitive mild traumatic brain injury; hippocampus; microgliosis; astrogliosis; quercetin.

References

  1. Centers for Disease Control and Prevention. TBI: Get the Facts. 2016 [updated 2016 Jan 22; cited 2016 Mar 15]; Available from: www.cdc.gov/traumaticbraininjury/get_the_facts.html
  2.  
  3. Frey WF, Savage RC. The road to rehabilitation: Part 8 – Journey Toward Understanding: Concussion & Mild Brain Injury. Vienna: Brain Injury Association of America; 2001.
  4.  
  5. Blyth BJ, Bazarian JJ. Traumatic alterations in consciousness: traumatic brain injury. Emerg Med Clin North Am. 2010;28(3):571-94. CrossRef PubMed PubMedCentral
  6.  
  7. Kay T, Harrington D, Adams R, Anderson T, Berrol S, Cicerone K et al. Definition of mild traumatic brain injury. J Head Traum Rehab. 1993;8(3):86-7. CrossRef  
  8. Blennow K, Hardy J, Zetterberg H. The neuropathology and neurobiology of traumatic brain injury. Neuron. 2012;76(5):886-99. CrossRef PubMed
  9.  
  10. Gourley MM, Valovich McLeod TC, Bay RC. Awareness and recognition of concussion by youth athletes and their parents. Athl Train Sport Heal Care. 2010;2(5):208-18. CrossRef  
  11. Jordan BD. The clinical spectrum of sport-related traumatic brain injury. Nature reviews. Neurology. 2013;9(4):222-30.
  12.  
  13. McKee AC, Cantu RC, Nowinski CJ, Hedley-Whyte ET, Gavett BE, Budson AE, et al. Chronic traumatic encephalopathy in athletes: progressive tauopathy after repetitive head injury. J Neuropathol Exp Neurol. 2009;68(7):709-35. CrossRef PubMed PubMedCentral
  14.  
  15. Daneshvar DH, Riley DO, Nowinski CJ, McKee AC, Stern R a, Cantu RC. Long-term consequences: effects on normal development profile after concussion. Phys Med Rehabil Clin N Am. 2011;22(4):683-700. CrossRef PubMed PubMedCentral
  16.  
  17. McKee AC, Robinson ME. Military-related traumatic brain injury and neurodegeneration. Alzheim Dement. 2014;10(3 Suppl):242-53.
  18.  
  19. Granacher R. Traumatic brain injury: methods for clinical and forensic neuropsychiatric assessment. 3rd ed. Boca Raton: CRC Press; 2015.
  20.  
  21. Xiong Y, Mahmood A, Chopp M. Animal models of traumatic brain injury. Nat Rev Neurosci. 2013;14(2):128-42. CrossRef PubMed PubMedCentral
  22.  
  23. Shultz SR, Bao F, Omana V, Chiu C, Brown A, Cain DP. Repeated mild lateral fluid percussion brain injury in the rat causes cumulative long-term behavioral impairments, neuroinflammation, and cortical loss in an animal model of repeated concussion. J Neurotrauma. 2012;29(2):281-94. CrossRef PubMed
  24.  
  25. Barth J, Freeman JR, Broshek DK. Mild head injury. In: Ramachandran VS, editor. Encyclopedia of human brain. San Diego: Academic Press; 2002. p. 81-92.
  26.  
  27. Gay MR, Rosenthal SL. Current understanding of concussion: treatment perspectives. In: Slobounov SM, Sebastianelli WJ, editors. Concussions in athletics: from brain to behavior. New York: Springer; 2014.
  28.  
  29. Werner C, Engelhard K. Pathophysiology of traumatic brain injury. Br J Anaesth. 2007;99(1):4-9. CrossRef PubMed
  30.  
  31. Slemmer JE, Shacka J, Weber JT. Antioxidants and free radical scavengers for the treatment of stroke, traumatic brain injury and aging. Curr Med Chem. 2008;15:404-14. CrossRef PubMed
  32.  
  33. Havsteen BH. The biochemistry and medical significance of the flavonoids. Pharmacol Ther. 2002;96(2-3):67-202.
  34.  
  35. Middleton Jr E, Kandaswami C, Theoharides TC. The effects of plant flavonoids on mammalian cells: implications for inflammation, heart disease, and cancer. Pharmacol Rev. 2000;52(4):673-751. Williams RJ, Spencer JPE, Rice-Evans C. Flavonoids: antioxidants or signalling molecules? Free Radic Biol Med. 2004;36(7):838-49. CrossRef PubMed
  36.  
  37. Kelly GS. Quercetin. Monograph. Altern Med Rev. 2011;16(2):172-9.
  38.  
  39. Cho J-Y, Kim I-S, Jang Y-H, Kim A-R, Lee S-R. Protective effect of quercetin, a natural flavonoid against neuronal damage after transient global cerebral ischemia. Neurosci Lett. 2006;404(3):330-5. CrossRef PubMed
  40.  
  41. Schültke E, Kamencic H, Skihar VM, Griebel R, Juurlink B. Quercetin in an animal model of spinal cord compression injury: correlation of treatment duration with recovery of motor function. Spinal Cord. 2010;48(2):112-7. CrossRef PubMed
  42.  
  43. Kovalenko TM, Osadchenko IO, Tsupykov OM, Pivneva TA, Shalamaĭ AS, Moĭbenko OO, Skybo HH. Neuroprotective effect of quercetin during experimental brain ischemia. Fiziol Zh. 2006;52(5):21-7 [Ukrainian].
  44.  
  45. Kane MJ, Angoa-Perez M, Briggs DI, Viano DC, Kreipke CW, Kuhn DM. A mouse model of human repetitive mild traumatic brain injury. J Neurosci Methods. 2012; 203(1):41-9. CrossRef PubMed PubMedCentral
  46.  
  47. Marmarou A, Foda MA, van den Brink W, Campbell J, Kita H, Demetriadou K. A new model of diffuse brain injury in rats. Part I: pathophysiology and biomechanics. J Neurosurg. 1994;80(2):291-300. CrossRef PubMed
  48.  
  49. Pivneva TA, Tsupikov OM, Pilipenko MN, Vasilenko DA, Skibo GG. Structural modifications of astrocytes in the hippocampus after experimental cerebral ischemia in gerbils. Neurophysiology. 2005;37(5):359-64. CrossRef  
  50. Zabenko Y, Pivneva T. Behavioral reactions and structural alterations of hippocampal tissue after repetitive mild traumatic brain injury in mice. Biologia. 2014; 59(2): 63-71.
  51.  
  52. Pannese E. Neurocytology: fine structure of neurons, nerve processes, and neuroglial cells. 2nd edition. Springer. 2015.
  53.  
  54. Loane DJ, Kumar A. Microglia in the TBI brain: the good, the bad, and the dysregulated. Exp Neurol. 2016; 275 (3): 316-27.
  55.  
  56. Kettenmann H, Hanisch U-K, Noda M, Verkhratsky A. Physiology of microglia. Physiol Rev. 2011;91(2):461- 553. CrossRef PubMed
  57.  
  58. Beauchamp K, Mutlak H, Smith WR, Shohami E, Stahel PF. Pharmacology of traumatic brain injury: where is the "golden bullet"? Mol Med. 2008;14(11-12):731-740.
  59.  
  60. Saija A, Scalese M, Lanza M, Marzullo D, Bonina F, Castelli F. Flavonoids as antioxidant agents: importance of their interaction with biomembranes. Free Radic Biol Med. 1995;19(4):4816. CrossRef  

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