Українська English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2016; 62(5): 12-18


О.P. Кolesnyk, S.А.Fedulova, N.S. Veselovsky

    O.O. Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv


Changes in amplitudes of evoked inhibitory postsynaptic currents (eIPSCs) from rat cultured hippocampal neurons were studied using whole-cell patch-clamp technique in postsynaptic neuron and local extracellular electrical paired pulse stimulation of single presynaptic axon. Paired pulse depression (PPD) and paired pulse facilitation (PPF) were observed in studied 43 neurons. According to coefficient of variance (CV) analysis was found that CV of second respond was significantly larger than CV first by 57 % (n = 26) during depression and significanty smaller by 27 % (n = 17) during facilitation. Thus, only presynaptic mechanism underlies shortterm depression and facilitation. We also estimated quantal parameters assuming that transmitter release is reasonably described by a binomial distribution. We found that under our experimental conditions there were no significant changes in GABA release probability during PPD and PPF. The values of mean quantal content and mean number of sites of transmitter release were the same for first stimulus in pair and were equal 9.7 in depression and 5 in facilitation of synaptic transmission. The values both of them for second stimulus were significant decreased by 28 and 26% during PPD and were significant increased by 14 and 11% during PPF, respectively. Thus, there is significant difference in quantal parameters characterizing GABA release during short-term depression and facilitation of synaptic transmission.

Keywords: PPD and PPF of GABAergic synaptic transmission; interstimulus interval; coefficient of variation.


  1. Jensen K, Lambert JD, Jensen MS. Activity-dependent depression of GABAergic IPSCs in cultured hippocampal neurons. J Neurophysiol. 1999; 82: 42-9.
  3. Wu LG, Borst JG. The reduced release probability of releasable vesicles during recovery from short-term synaptic depression. Neuron. 1999; 23: 821–32. CrossRef  
  4. Zucker RS., Regehr WG. Short-term synaptic plasticity. An Rev Physiol. 2002; 64: 355–405. CrossRef PubMed
  6. Dobrunz LE and Stevens CF. Heterogeneity of release probability, facilitation, and depletion at central synapses. Neuron. 1997; 6: 995-1008. CrossRef  
  7. Wilcox KS, Dichter MA. Paired pulse depression in cultured hippocampal neurons is due to a presynaptic mechanism independent of GABAB autoreceptor activation. J Neurosci. 1994; 14: 1775-88.
  9. Fedulova SA, Veselovsky NS. Quantal GABA release in hippocampal synapses: role of local Ca2+ dynamics within the single terminals. Eur J Pharmacol. 2002; 2/3: 163–71.
  11. Veselovsky N S, Engert F, Lux H D.Fast local superfusion technique. Pflüg Arch. 1996; 432: 351-4. CrossRef  
  12. Mizerna OP, Fedulova SA, Veselovsky MS. Effect of thapsigargin on inhibitory synaptic transmission between cultured neurons of the rat hippocampus. Neurophysiology. 2007; 39(4-5): 374-6.
  14. Faber DS, Korn H. Applicability of the coefficient of variation method for analyzing synaptic plasticity. Biophys. J. 1991; 60: 1288-94.
  16. Larkman A, Hannay T, Stratford K, Jack J. Presynaptic release probability influences the locus of long-term potentiation. Nature. 1992; 360: 70-3. CrossRef PubMed
  18. Sjostrom PJ, Turrigiano GG, Nelson SB. Multiple forms of long-term plasticiry at unitary neocortical layer 5 synapses. Neuropharmacology. 2007; 52: 176-84. CrossRef PubMed
  20. Debanne D, Guerineau NC, Gahwiler BH, Thompson SM. Paired-pulse facilitation and depression at unitary synapses in rat hippocampus: quantal fluctuation affects subsequent release. J Physiol. 1996; 491: 163-76. CrossRef PubMed PubMedCentral
  22. Oleskevich S, Clements J, Walmsley B. Release probability modulates short-term plasticity at a rat giant terminal. J Physiol. 2000; 524: 513-23. CrossRef PubMed PubMedCentral

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2024.