REDUCED RESPONSE OF NATURAL KILLER LYMPHOCYTES TO TOLL-LIKE RECEPTOR 3 STIMULATION IN CHILDREN WITH RECURRENT INFECTIONS
D.V. Osypchuk1 , V.P. Chernyshov1 , L.I. Chernysheva2 , N.P. Kissel3, B.V. Dons`koi1, I.M. Matvienko4, V.P. Rodionov4, Yu.A. Makovs`ka4
- Laboratory of Immunology, Institute of Pediatrics,
Obstetrics and Gynecology, National Academy of Medical
Sciences of Ukraine, Kiev.Ukraine
- Department of Children Infectious Diseases and Children’s
Immunology, Shupyk National Medical Academy of
Postgraduate Education, Kiev.Ukraine
- Department of septic surgery, National Children’s
Specialized Hospital «OHMATDYT», Kiev.Ukraine
- Department of health child’s problems and premorbid
states, Institute of Pediatrics, Obstetrics and Gynecology,
National Academy of Medical Sciences of Ukraine.
DOI: https://doi.org/10.15407/fz62.04.012
Abstract
In this study, we examined the activation of natural killer(NK)-
lymphocytes mediated by Toll-like receptor 3(TLR3), in the
group of children with recurrent infections and the group of
children with invasive bacterial infections. We examined
level of CD69 (marker of activation) expression on NK -
lymphocytes after incubation with TLR3 ligand. There was
a significant decrease in the level of an activation marker –
36,3±4,4% in the group of children with recurrent infections,
compared with a control group of healthy children (56,5±4,9%)
and the group of children with invasive bacterial infections
(55,9±4,4%). Also, decreased was an activation potential
of NK-lymphocytes - 24,9±4,5% which was calculated
as the difference between the percentage of CD69+ NK -
lymphocytes after incubation and spontaneous value (without
the addition of activator). There were no correlations between
markers of TLR3 mediated activation of NK-cells and the
age. Thus, the ability to activate NK - lymphocyte mediated
by TLR3, independent of age and reduced in the group of
children with recurrent infections. Reduced activation of
NK-lymphocytes may contribute to increased susceptibility
to viral infections and bacterial complications.
Keywords:
NK-cells; TLR; Toll-like receptors.
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