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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2016; 62(4): 12-17

REDUCED RESPONSE OF NATURAL KILLER LYMPHOCYTES TO TOLL-LIKE RECEPTOR 3 STIMULATION IN CHILDREN WITH RECURRENT INFECTIONS

D.V. Osypchuk1 , V.P. Chernyshov1 , L.I. Chernysheva2 , N.P. Kissel3, B.V. Dons`koi1, I.M. Matvienko4, V.P. Rodionov4, Yu.A. Makovs`ka4

  1. Laboratory of Immunology, Institute of Pediatrics, Obstetrics and Gynecology, National Academy of Medical Sciences of Ukraine, Kiev.Ukraine
  2. Department of Children Infectious Diseases and Children’s Immunology, Shupyk National Medical Academy of Postgraduate Education, Kiev.Ukraine
  3. Department of septic surgery, National Children’s Specialized Hospital «OHMATDYT», Kiev.Ukraine
  4. Department of health child’s problems and premorbid states, Institute of Pediatrics, Obstetrics and Gynecology, National Academy of Medical Sciences of Ukraine.
DOI: https://doi.org/10.15407/fz62.04.012


Abstract

In this study, we examined the activation of natural killer(NK)- lymphocytes mediated by Toll-like receptor 3(TLR3), in the group of children with recurrent infections and the group of children with invasive bacterial infections. We examined level of CD69 (marker of activation) expression on NK - lymphocytes after incubation with TLR3 ligand. There was a significant decrease in the level of an activation marker – 36,3±4,4% in the group of children with recurrent infections, compared with a control group of healthy children (56,5±4,9%) and the group of children with invasive bacterial infections (55,9±4,4%). Also, decreased was an activation potential of NK-lymphocytes - 24,9±4,5% which was calculated as the difference between the percentage of CD69+ NK - lymphocytes after incubation and spontaneous value (without the addition of activator). There were no correlations between markers of TLR3 mediated activation of NK-cells and the age. Thus, the ability to activate NK - lymphocyte mediated by TLR3, independent of age and reduced in the group of children with recurrent infections. Reduced activation of NK-lymphocytes may contribute to increased susceptibility to viral infections and bacterial complications.

Keywords: NK-cells; TLR; Toll-like receptors.

Full text: (PDF, in Ukrainian)

References

  1. O'Neill LA. The interleukin-1 receptor/Toll-like receptor superfamily: 10 years of progress. Immunol Rev.2008; 226:10–8. CrossRef PubMed
    2.  
  2. Berezhnaia NM, Sepiashvili RI. Physiology of Toll-like receptors--regulators of natural and acquired immunity. Fiziol Zh. 2011;57(5):26-9. CrossRef PubMed
    3.  
  3. Fitzner N, Clauberg S, Essmann F, Liebmann J, KolbBachofen V. Human skin endothelial cells can express all 10 TLR genes and respond to respective ligands. Clin. Vaccine Immunol.2008;15(1):138-46. CrossRef PubMed PubMedCentral
    4.  
  4. Ingerslev HC, Ossum CG, Lindenstrem T, Nielsen ME. Fibroblasts express immune relevant genes and are important sentinel cells during tissue damage in rainbow trout. PLoS One.2010;5(2):e9304. CrossRef PubMed PubMedCentral
    5.  
  5. Trinchieri G, Sher A. Cooperation of Toll-like receptor signals in innate immune defence Nat Rev Immunol.2007;7(3):179-90. CrossRef PubMed
    6.   Sun J C, Lanier L L. NK cell development, homeostasis and function:Parallels with CD8 T cells. Nat Rev Immunol.2011;11: 645–57 CrossRef PubMed PubMedCentral  
  6. Lauzon NM, Mian F, MacKenzie R, Ashkar AA. The direct effects of Toll-like receptor ligands on human NK cell cytokine production and cytotoxicity. Cell Immunol.2006;241:102–12. CrossRef PubMed
    7.   Wulff S, Pries R, Wollenberg B. Cytokine release of human NK cells solely triggered with Poly I:C Cell Immunol. 2010;263(2):135-7 CrossRef PubMed  
  7. Moore TC, Kumm PM, Brown DM, Petro TM. Interferon response factor 3 is crucial to poly-I:C induced NK cell activity and control of B16 melanoma growth. Cancer Lett. 2014;346(1):122-8. CrossRef PubMed PubMedCentral
    8.   Tyler C. Moore, Phyllis M. Kumm, Deborah M. Brown, Thomas M. Petro. Souza-Fonseca-Guimaraes F, AdibConquy M, Cavaillon JM. Natural Killer (NK) Cells in Antibacterial Innate Immunity: Angels or Devils? Mol Med.2012;30:270-85  
  8. Adib-Conquy M1, Scott-Algara D2, Cavaillon JM1, Souza-Fonseca-Guimaraes F TLR-mediated activation of NK cells and their role in bacterial/viral immune responses in mammals Immunol Cell Biol. 2014 Mar;92(3):256-62. CrossRef PubMed
    9.  
  9. Dong Z, Wei H, Sun R, Hu Z, Gao B, Tian Z.Involvement of natural killer cells in PolyI:C-induced liver injury. J Hepatol. 2004;41(6):966-73. CrossRef PubMed
    10.  
  10. Sarris M, Masson JB, Maurin D, Van der Aa LM, Boudinot P, Lortat-Jacob H, Herbomel P. Unni AM, Bondar T. Inflammatory chemokines direct and restrict leukocyte migration within live tissues as glycan-bound gradients. Curr Biol. 2012;22(24):2375-82. CrossRef PubMed
    11.  
  11. Kollmann TR, Levy O, Montgomery RR, Goriely S. Innate immune function by Toll-like receptors: distinct responses in newborns and the elderly. Immunity. 2012;37(5):771-83. CrossRef PubMed PubMedCentral
    12.  
  12. Medzhitov R. Intrinsic sensor of oncogenic transformation induces a signal for innate immunosurveillance. Proc Natl Acad Sci U S A. 2008; 105:1686–91. CrossRef PubMed PubMedCentral
    13.  
  13. Zeng R, Li C, Li N, Wei L, Cui Y. The role of cytokines and chemokines in severe respiratory syncytial virus infection and subsequent asthma. Cytokine. 2011;53(1):1-7. CrossRef PubMed
    14.  
  14. Heltzer ML, Coffin SE, Maurer K, Bagashev A, Zhang Z, Orange JS, Sullivan KE. Immune dysregulation in severe influenza. J Leukoc Biol. 2009;85(6):1036-43. CrossRef PubMed PubMedCentral
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