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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2016; 62(4): 12-17


REDUCED RESPONSE OF NATURAL KILLER LYMPHOCYTES TO TOLL-LIKE RECEPTOR 3 STIMULATION IN CHILDREN WITH RECURRENT INFECTIONS

D.V. Osypchuk1 , V.P. Chernyshov1 , L.I. Chernysheva2 , N.P. Kissel3, B.V. Dons`koi1, I.M. Matvienko4, V.P. Rodionov4, Yu.A. Makovs`ka4

  1. Laboratory of Immunology, Institute of Pediatrics, Obstetrics and Gynecology, National Academy of Medical Sciences of Ukraine, Kiev.Ukraine
  2. Department of Children Infectious Diseases and Children’s Immunology, Shupyk National Medical Academy of Postgraduate Education, Kiev.Ukraine
  3. Department of septic surgery, National Children’s Specialized Hospital «OHMATDYT», Kiev.Ukraine
  4. Department of health child’s problems and premorbid states, Institute of Pediatrics, Obstetrics and Gynecology, National Academy of Medical Sciences of Ukraine.
DOI: https://doi.org/10.15407/fz62.04.012

Abstract

In this study, we examined the activation of natural killer(NK)- lymphocytes mediated by Toll-like receptor 3(TLR3), in the group of children with recurrent infections and the group of children with invasive bacterial infections. We examined level of CD69 (marker of activation) expression on NK - lymphocytes after incubation with TLR3 ligand. There was a significant decrease in the level of an activation marker – 36,3±4,4% in the group of children with recurrent infections, compared with a control group of healthy children (56,5±4,9%) and the group of children with invasive bacterial infections (55,9±4,4%). Also, decreased was an activation potential of NK-lymphocytes - 24,9±4,5% which was calculated as the difference between the percentage of CD69+ NK - lymphocytes after incubation and spontaneous value (without the addition of activator). There were no correlations between markers of TLR3 mediated activation of NK-cells and the age. Thus, the ability to activate NK - lymphocyte mediated by TLR3, independent of age and reduced in the group of children with recurrent infections. Reduced activation of NK-lymphocytes may contribute to increased susceptibility to viral infections and bacterial complications.

Keywords: NK-cells; TLR; Toll-like receptors.

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