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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2016; 62(3): 39-47


M.N. Khodosovsky

    Grodno State Medical University, Belarus


The effect of hypoxic preconditioning (HP) on the blood oxygen-binding properties and liver oxidative damages was determine during hepatic ischemia-reperfusion (HIR) in rabbits. Animals were randomized into two experimental groups: 1st (HIR) - hepatic ischemia (30 min by Pringle maneuver) and reperfusion (120 min); 2nd (HP+HIR) – before HIR the rabbits were passed through hypoxic chamber at 3500 m altitude during 1 hr/day (3 times day after day). The parameters of blood oxygen transport (р50, рСО2, рО2, рН, HCO3 - , ABE and ect.), lipid peroxidation products (conjugated dienes, Schiff bases) and blood hepatic markers (ALT, AST) were detected. It’s found that HIR leads to decline in hemoglobin oxygen affinity, activation of lipid peroxidation processes and elevation of ALT and AST activities in the 1st group. Hypoxic preconditioning (2nd group) markedly increased hemoglobin oxygen affinity, reduced the lipid peroxidation processes and ALT and AST activities in the blood during HIR. Thus, HP has a protective effect during HIR through elevation of hemoglobin oxygen affinity and declining hepatic oxidative damages.

Keywords: oxygen; hypoxia; preconditioning; liver; ischemia; reperfusion; rabbits.


  1. Choukèr A, Ohta A, Martignoni A, Lukashev D, Zacharia LC, Jackson EK, et al. In vivo hypoxic preconditioning protects from warm liver ischemia-reperfusion injury through the adenosine A2B receptor. Transplantation. 2012; 94(9):894-902. CrossRef PubMed PubMedCentral
  3. Czigány Z, Turóczi Z, Ónody P, Harsányi L, Lotz G, Hegedüs V, et al. Remote ischemic perconditioning protects the liver from ischemia-reperfusion injury. J Surg Res 2013; 185(2):605-13. CrossRef PubMed
  5. Alchera E, Dal Ponte C, Imarisio C, Albano E, Carini R. Molecular mechanisms of liver preconditioning. World J Gastroenterol. 2010; 16(48):6058-67. CrossRef PubMed PubMedCentral
  7. Song X, Zhang N, Xu H, Cao L, Zhang H. Combined preconditioning and postconditioning provides synergistic protection against liver ischemic reperfusion injury. Int J Biol Sci. 2012; 8(5):707-18. CrossRef PubMed PubMedCentral
  9. Kageyama S, Hata K, Tanaka H, Hirao H, Kubota T, Okamura Y, et al. Intestinal ischemic preconditioning ameliorates hepatic ischemia-reperfusion injury in rats: role of heme oxygenase 1 in the second window of protection. Liver Transpl. 2015; 21(1):112-22. CrossRef PubMed
  11. Lai IR, Ma MC, Chen CF, Chang KJ. The protective role of heme oxygenase-1 on the liver after hypoxic preconditioning in rats. Transplantation. 2004; 77(7):1004-8. CrossRef PubMed
  13. Maslov LN, Lishmanov Yu B, Kolar F, Portnichenko AG, Podoksenov Yu.K, Khaliulin I G, et al. Hypoxic preconditioning is a phenomenon increa-sing tolerance of cardiomyocytes to hypoxia-reoxygenation. Ross Fiziol Zh Im I M Sechenova. 2010; 96(12):1170-89. [Russian]. PubMed
  15. Samoĭlov MO, Rybnikova EA, Churilova AV. Signal molecular and hormonal mechanisms of formation of the hypoxic preconditioning protective effects. Patol Fiziol Eksp Ter. 2012; (3): 3-10. PubMed
  17. Simonson TS, Wei G, Wagner HE, Wuren T, Bui A, Fine JM, et al. Increased blood-oxygen binding affinity in Tibetan and Han Chinese residents at 4200 m. Exp Physiol. 2014; 99(12):1624-35. CrossRef PubMed PubMedCentral
  19. Khodosovskiĭ MN, Zinchuk VV. Erythropoietin influence on the blood oxygen transport and prooxidant-antioxidant state during hepatic ischemia-reperfusion. Ross Fiziol Zh Im I M Sechenova. 2014;100(5):592-601. PubMed
  21. Tin'kov A.N., Prokof'ev A.B., Grintsova M.V. Evaluation of indices of cardiac electrical instability in patients with myocardial infarction during out-patient stage of followup care using the altitute-chamber hypoxia technique . Journal of Arrhythmology. 2005; (39):31-4.
  23. Gavrilov VB, Gavrilova AR, Khmara NF. Measurement of diene conjugates in blood plasma using the UV absorption of heptane and isopropanol extracts. Lab Delo. 1988; (2):60-4. PubMed
  25. Fletcher BL, Dillard CJ, Tappel AL. Measurement of fluorescent lipid peroxidation products in biological systems and tissues. Anal Biochem. 1973; 52(1): 1-9. CrossRef  
  26. Bilenko M.V. Ischemic and reperfusion injuries of organs (molecular mechanisms and ways to prevention and treatment). Moscow: Medicine; 1989. [Russian].
  28. Hummitzsch L, Zitta K, Bein B, Steinfath M, Albrecht M. Culture media from hypoxia conditioned endothelial cells protect human intestinal cells from hypoxia- reoxygenation injury. Exp Cell Res. 2014; 322(1):62-70. CrossRef PubMed
  30. Akhtar MZ, Sutherland AI, Huang H, Ploeg RJ, Pugh CW. The role of hypoxia-inducible factors in organ donation and transplantation: the current perspective and future opportunities. Am J Transplant. 2014; 14(7):1481-7. CrossRef PubMed
  32. Zhong Z, Ramshesh VK, Rehman H, Currin RT, Sridharan V, Theruvath TP, et al. Activation of the oxygen-sensing signal cascade prevents mitochondrial injury after mouse liver ischemia-reperfusion. Am J Physiol Gastrointest Liver Physiol. 2008; 295(4):G823-32. CrossRef PubMed PubMedCentral
  34. Crocker GH, Toth B, Jones JH. Combined effects of inspired oxygen, carbon dioxide, and carbon monoxide on oxygen transport and aerobic capacity. J Appl Physiol(1985). 2013; 115(5):643-52. CrossRef PubMed

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