PECULIARITIES OF THE INFLUENCE OF STREPTOZOTOCIN-INDUCED DIABETIS AND INCOMPLETE ISCHEMIAREPERFUSION OF THE BRAIN DURING APOPTOSIS OF VARIOUS NEOCORTICAL LOBES OF RATS
T.M. Boychuk,T.I. Kmet
Higher State Educational Establishment of Ukraine «Bukovinian State Medical University», Chernivtsi
The effect of diabetes mellitus on the dynamics of neurocyte
and gliacyte apoptosis intensity in the cortex of the frontal,
parietal and temporal lobes of the cerebral hemispheres under
conditions of ischemic-reperfusion lesion has been studied in
experiments on rats. The level of apoptotic processes in the
neuro- and gliacytes of the frontal cortex has been found to
be unchanged after 20 minutes of carotid ischemia followed
by one hour reperfusion according to the indices examined in
animals with out diabetes mellitus. Apoptosis of neurocytes
is activated in the cortex of the parietal lobe, and that of the
neuro- and gliacytes – in the cortex of the temporal lobe.
Three-month diabetes mellitus intensifies apoptosis of neurons
and glial cells in the cortex of the frontal and temporal lobes,
neurons in the cortex of the parietal lobe and decreases apoptosis
of gliacytes in it. In early ischemic-reperfusion period
the activity of apoptotic processes in the cortex of the frontal
and temporal lobes does not change in animals with diabetes
mellitus, but it decreases in the cortex of the parietal lobe at
the expense of glial cells. On the 12th day of observation the
activity of apoptotic processes in neurocytes of the cortex of the
temporal lobe increases in rats without diabetes mellitus, and
it decreases in the glial cells. We detected a reduced content of
the protein p53 in neurons and increased density of р53+-cells.
In this period of observation in rats with diabetes mellitus the
activity of apoptotic processes decreases in general both in
neurons and glial cells of all the lobes. The results obtained
point for the availability of regional differences in the dynamics
of reaction of the cerebral hemisphere lobes in response to
ischemic-reperfusion injury charachterized by the intensity of
apoptosis of neurons and glial cells. The results also point for
modifying effect of diabetes mellitus on the indices studied.
diabetes mellitus; ischemia-reperfusion of the brain; cerebral cortex; apoptosis
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