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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2016; 62(2): 35-40


Surface charge impact in nonsynaptic model of epilepsy in rat hippocampus

O.S. Zapukhliak, V.О. Kachanovska, E.V. Isaeva, O.V. Netsyk, D.S. Isaev

    O.O.Bogomoletz Institute of Physiology of National Academy of Science of Ukraine, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz62.02.035

Abstract

Decreasing of surface charge screening near voltage-gated ion channels via reduction of extracellular cation divalent ions provide potent mechanism of altering cellular excitability and seizure threshold. Spontaneous field potentials were recorded from horizontal brain slices of young Wistar rats (postnatal day 10-12). Extracellular registrations wereobtained from CA1 and CA3 area of hippocampus. For induction of nonsynaptic epileptiform activity slices were perfused with artificial cerebrospinal fluid with omitted Ca2+and Mg2+ ions. Effect of different Mg2+ concentration (1, 2, and 3mmol/l) on initial stage of nonsynaptic epileptiform discharges was studied. Our results suggest that the change in Mg2+ concentration dramatically affects the probability of induction of low-Ca2+seizure-like activity (SLA), providing evidence that Mg2+ can alter cerebral excitability by affecting the surface charge and supporting the idea that surface charge could be a pharmacological target for anti-epileptic treatment.

Keywords: low-Ca2+ seizure-like activity; surface charge; magnesium; hippocampus.

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