EXCITABILITY PROPERTIES OF TRIGEMINAL GANGLION NEURONS
M.V. Telka, O.V. Rikhalsky, N.S.Veselovsky
O.O.Bogomoletz Institute of Physiology of the National
Academy of Sciences of Ukraine, Kyiv, Ukraine.
DOI: https://doi.org/10.15407/fz62.02.024
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Abstract
The firing properties of small neurons (with diameters of
soma less than 25 µm) were investigated using patch-clamp
technique in whole-cell configuration in primary culture of
trigeminal ganglia (TG) of postnatal rats. TG neurons were
divided into three groups according to their firing responses
to long-lasting depolarizing pulses: adaptive neurons (AN)
characterized by a strongly adaptive responses; tonic neurons
(TN) characterized by a multiple tonic firing; neurons with a
delay before initiation of AP generation, namely, NDG. AN,
TN and NDG also differed in AP electrophysiological and
pharmacological characteristics. TN was distinguished by
responses to hyperpolarization and the greatest value of input
resistance. TN, AN and NDG were characterized by different
active properties (amplitude of action potential and afterhyperpolarization,
reobase, threshold). Each group of neurons was
characterized by heterogeneity of AP duration and of frequency
properties for TN. The application of tetrodotoxin (TTX) (250
nM) resulted in full or partial inhibition of AP generation and
some neurons had TTX – insensitive firing responses. Neurons
that were not affected by TTX had markedly longer AP. TTX
had no effect on electrical activity of some AN and NDG.
Based on sensitivity to TTX and their electrophysiological
properties, AN and NDG seem to be C-fiber nococeptors.
Keywords:
trigeminal ganglion neurons; primary culture; types of firing response; parameters action potentials; sensitivity to tetrodotoxin (TTX).
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