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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2015; 61(6): 119-128


EFFECT OF HYPOXIA ON SYNAPTIC TRANSMISSION BETWEEN RETINAL GANGLION CELLS AND SUPERIOR COLLICULUS NEURONS IN COCULTURE

G.V. Dumanska, O.V. Rikhalsky, N.S. Veselovsky

  1. O.O.Bogomoletz Institute of Physiology of the National Academy of Sciences of Ukraine, Kyiv;
  2. International Center for molecular Physiology of the National Academy of Sciences of Ukraine, Kyiv
DOI: https://doi.org/10.15407/fz61.06.119

Abstract

In this study we conducted a series of experiments to characterize the effect and define the mechanisms of hypoxia on synaptic transmission between retinal ganglion cells and superior colliculus (SC) neurons. Application of hypoxic solution leads to a long lasting potentiation (LTP) NMDA-mediated synaptic transmission. Analysis of the oxygen deficiency effect on the spontaneous and miniature postsynaptic currents (sPSC and mPSC respectively) revealed an increase in the frequency of their occurrence and the appearance of the second peak in the mPSC histogram distribution. The assessment of quantum and binomial parameters reflects the complex pre- and postsynaptic changes during the potentiation, independent of the release probability. Most likely this LTP can be caused by an increase in the total number of active synapses. Glutamatergic synaptic transmission mediated by non-NMDA activation receptor-channel complexes, responded to application of deoxygenated solution by the brief depression, which is the result of presynaptic dysfunction and associates with decrease in release probability and number of active zones. GABAergic synaptic transmission mediated by activation GABAA-receptor-channel complexes, responded to hypoxic action by long term depression (LTD). Analysis of sPSC and mPSC showed a significant decrease in the frequency of their occurrence and significant (P = 0,05) decrease in the quantum over a period of oxygen deficiency. In general, the effect of hypoxia-induced LTD of GABAergic synaptic transmission is based on complex changes of presynaptic (independent on the release probability) and postsynaptic (reduction sensitivity of receptors in postsynaptic membrane) mechanisms.

Keywords: retinal ganglion cells; superior colliculus neurons; synaptic transmission; hypoxia; long-term potentiation and depression.

References

  1. Pike MG, Holmstrom G, de Vries LS. Patterns of visual impairment associated with lesions of the preterm infant brain. Dev Med Child Neurol. 1994;36: 849–862. CrossRef PubMed
  2.  
  3. Huo R, Burden S, Hoyt CS, Good WV. Chronic cortical visual impairment in children: etiology, prognosis, and associated neurological deficits. Br J Ophthalmol. 1999;83:670–675. CrossRef PubMed PubMedCentral
  4.  
  5. Hoyt CS. Visual function in the brain-damaged child. Eye. 2003; 17: 369–384. CrossRef PubMed
  6.  
  7. Dutton GN, McKillop ECA, Saidkasimova A. Visual problems as a result of brain damage in children. Br J Ophthalmol. 2006; 90: 932–933. CrossRef PubMed PubMedCentral
  8.  
  9. Zhou IY, Wu EX, Cheung MM, Chan KC, Xing KK. Functional MRI of postnatal visual development in normal and hypoxic-ischemic-injured superior colliculi. Neuroimage. 2010;49(3):2013-2020. CrossRef PubMed
  10.  
  11. Chan KC, Kancherla S, Fan S-J, Wu EX. Long-term effects of neonatal hypoxia-ischemia on structural and physiological integrity of the eye and visual pathway by multimodal MRI. Invest Opthalmol Vis Sci. 2015;56:1-9.
  12.  
  13. Dumanska GV, Kosheleva SO, Veselovsky MS. Chemical transmission between cocultivated ratinal ganglion cells and superior colliculus neurons. Neurophysiol. 2011;43(4):369-371.
  14.  
  15. Veselovsky NS, Engert F, Lux HD. Fast local superfusion technique. Pflugers Arch. 1996; 432(2):351-4. CrossRef PubMed
  16.  
  17. Dumanska HV, Rykhalskyi OV, Veselovskyi MS. Characteristics of quantal release of glutamate and GABA in synapses between retinal ganglion cells and superior colliculus neurons in coculture. Fiziol Zh. 2014;60(1):3-10.
  18.  
  19. Sola E, Prestori F, Rossi P. et. al. Increased neurotransmitter release during long-term potentiation at mossy fibre-granule cell synapses in rat cerebellum. J. Physiol. 2004;557:843-861. CrossRef PubMed PubMedCentral
  20.  
  21. Faber VS and Korn H. Applicability of the coefficient of variation method for analyzing synaptic plasticity. J Biophys. 1991;60:1288-1294. CrossRef  
  22. Hori N, Carpenter DO. Functional and morphological changes induced by transient in vivo ischemia. Exp Neurol. 1994;129:279 –289. CrossRef PubMed
  23.  
  24. Hsu KS, Huang CC. Protein kinase C inhibitors block generation of anoxia-induced long-term potentiation. Neuroreport. 1998;9:3525–3529. CrossRef  
  25. Jourdain P, Nikonenko I, Alberi S, Muller D. Remodeling of hippocampal synaptic networks by a brief anoxiahypoglycemia. J Neurosci. 2002;22:3108 –3116.
  26.  
  27. Piccini A, Malinow R. Transient oxygen-glucose deprivation induces rapid morphological changes in rat hippocampal dendrites. Neuropharmacol. 2001;41:724 –729. CrossRef  
  28. Choi S, Lovinger DM. Decrease probability of neurotransmitter release underlies striatal long-term depression and postnatal development of corticostrial synapses. Proc Nat Acad Sci USA. 1997;94:2665-2670. CrossRef PubMed PubMedCentral
  29.  
  30. Geiman S. Heterogeneous release probability and activitydependent short-term synaptic depression. Commun Integr Biol. 2011;4(5):603-605. CrossRef  
  31. Kucukdereli H, Allen NJ, Lee ATl. Control of excitatory CNS synaptogenesis by astrocyte-secreted proteins Hevin and SPARC. PNAS. 2011;108( 32):440-449.
  32.  
  33. Vidal-Sanz M, Bray GM and Aguayo AJ. Regeneration synapses persist in the superior colliculus after the regrowth of retinal ganglion cell axons. J Neurocyt. 1991;20:940-952. CrossRef  

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