THE EFFECT OF DIETARY RESTRICTION DURING DEVELOPMENT OF DROSOPHILA MELANOGASTER ON THE ACTIVITY OF ANTIOXIDANT SYSTEM ENZYMES
O.G. Zabuga, A.K. Koliada, V.M. Kukharskyy, A.I. Bazhynova, A.M. Vaiserman
State Institution “D.F. Chebotarev Institute of Gerontology
NAMS of Ukraine”, Kyiv
In the previous study we demonstrated that dietary restriction
only at the development stage of Drosophila melanogaster
may impact the life span of adult flies. It was important that
we didn’t use qualitative (restriction of proteins or other
macro- or microelements) and not a calorie restriction as well,
but quantitative dietary restriction that was the proportional
reduction of all food components in the larval medium. In the
situations when the larvae were reared in the medium types,
that contained protein and carbohydrate components in concentrations
of 90-10% of food components compared to the
standard one (100%), the males were characterised with the
significant increase in the maximum life span. The average life
span was also increased, but only in those male individuals
that developed in the medium types, that contained 50% and
60% of food components compared to controls. Such an effect
we haven’t detected in the female flies.
To study the biochemical changes associated with the physiological
effects we have determined the activity of the antioxidant
enzymes − superoxide dismutase (SOD) and catalase. In
the male flies the 50% dietary restriction implemented during
the development has led to the significant increase in a SOD
and catalase activity. Also the flies of both sexes reared in the
medium with the 50% of food components have been characterised
with the reduction in the accumulation of glycation
end products. According to these results, we suggest that the
changes in the activity of antioxidant enzymes may play a
role in the increase of the flies life span caused by the dietary
restriction during the development.
Drosophila melanogaster; dietary restriction; development; superoxide dismutase; catalase; advanced glycation end products; life span.
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