MITOCHONDRIA RESPIRATION AND OXIDATIVE PHOSPHORILATION OF RAT TISSUES AT TAURINE PER ORAL INJECTION
R.D. Ostapiv1,2, V.V. Manko1
- Ivan Franko National University of Lviv;
- State Scientific Controlling Institute of Veterinary Medical
Products and Feed Additives, Lviv
Taurine – sulphur-containing amino acid is a necessary component
of mitochondrial matrix, where it maintains pH and is
included in mitochondrial transport RNA. But still it is unclear
how taurine influences on ATP synthesis and mitochondrial
respiration chain components activity. Thus, the aim of the
work was to study the effect of long-term per oral taurine injection
on mitochondrial respiration intensity in rat tissues: liver,
brain, testes and thigh muscle. For this purpose male Wistar
rats, that weighted 190–220 g, were divided in three groups,
daily during 28 days they were injected drinking water (control
group) or taurine solution 40 and 100 mg per kg of body
weight (І and ІІ research groups, correspondingly). Respiration
intensity was measured polarogrifically with use of Clark
electrode at endogenic substrates oxidation (V1), with exogenic
α-ketoglutarate (5 mmol/l) or succinate (1 mmol/l;VS
4 ) addition,
at ADP addition to concentration 200 µmol/l (V3), and
after ADP depletion (V4
АТP ). Phosphorylation time, oxidative
phosphorilation efficacy (АDP/О), respiratory controls by
4 ) and Chance (V3/ V4
АТP ) were calculated. It was
found that long term taurine injection increased V1 in animal
brain and liver of both research groups, but it decreased in
testes and muscles of I research group. In liver of I research
group animals, when both α-ketoglutarate and succinate were
4 , V3 and V4
АТP were 4–7 times larger than in
control. At the same time, Lardy respiratory control increased
at succinate oxidation, this may indirectly point on increased
coupling between respiration and oxidative phosphorylation.
In liver of II research group animals VS
4 , V3 and V4
α-ketoglutarate was oxidated were significantly higher than
in control. In muscles of I research group VS
4 , V3 and V4
when α-ketoglutarate and succinate was added were lower
than in control. In thigh muscle of II research group animals
at α-ketoglutarate oxidation V3 was higher than in control.
When succinate was added VS
4 and V4
АТP increased in testes
mitochondria of both research groups and in brain of I research
group. But in II research group animals mitochondria VS
brain was lower than in control. At the same time, coupling
between respiration and oxidative phosphorylation in brain was
on control level, in testes of I research group it was lower. In
testes of II research group animals at α-ketoglutarate addition
increased respiratory controls. Thus, the effect of long term per
oral taurine injection on mitochondria respiration intensity is
dose-dependent and tissue-specific and, obviously, has different
significance and is implemented by different mechanisms.
taurine; mitochondrial respiration intensity; liver; brain; testes; thigh muscle.
- Lambert IH, Kristensen DM, Holm JB. Physiological role of taurine – from organism to organelle. Acta Physiol. 2015;213:191–212.
- Hansen S, Birkedal H, Wibrand F. Taurine and regulation of mitochondrial metabolism. Adv Exp Med Biol. 2015;883(1):397–405.
- Palmi M, Youmbia GT, Fusia F. et al. Potentiation of mitochondrial Ca2+ sequestration by taurine. Biochem Pharm. 1999;58:1123–31.
- Ozasa H, Gould KG. Protective effect of taurine from osmotic stress on chimpanzee spermatozoa. Arch Androl. 1982;9:121–6.
- Xu S, He M, Zhong M, Wang S. et al. The neuroprotective effects of taurine against nickel by reducing oxidative stress and maintaining mitochondrial function in cortical neurons. Neu Let. 2015;590:52–57.
- Chou Ch, Lin H, Hwang L. Taurine resumed neuronal differentiation in arsenite‑treated N2a cells through reducing oxidative stress, endoplasmic reticulum stress, and mitochondrial dysfunction. Amino Acids. 2015;47(4):735–44.
- Jang J, Zong X, Wu G, Lin S. et al. Taurine increases testicular function in aged rats by inhibiting oxidative stress and apoptosis. Amino Acids. 2015;47(8):1549–1558.
- Zhang X, Shuo T, Wang Y, Xu B. Mechanism of taurineinduced apoptosis in human colon cancer cells. Acta Biochim Biophys Sin. 2014;46:261–272.
- Mu W, Zhang T, Jiang B. An overview of biological production of L-theanine. Biotech Adv. 2015;33:335–42.
- Scholz TD, Balaban RS. Mitochondrial Fl -ATPase activity of canine myocardium: effects of hypoxia and stimulation. Am J Physiol Heart Circ Physiol. 1994; 266:396 – 403.
- Kondrashova MN. Transeaminase cycle of substrate oxidation, as a mechanism to hypoxia adaptation. Pharmacol Corr Hypox Stag. 1987;1:51–70. [Russian].
- Della-Morte D, Kunjan RD, DeFazio AR. et al. Resveratrol pretreatment protects rat brain from cerebral ischemic damage via a SIRT1 – UCP2 pathway. Neuroscience. 2009;159(3): 993–1002.
- Shance B, Williams G. Respiratory enzymes in oxidative phosphorilation. J. Biol. Chem. 1955; 216: 383–393.
- Lowry OH, Rosebrough NJ, Fair AL. et al. Protein measurement with Folin phenol reagent. J Biol Chem. 1951;193:265–75.
- Derkach MP, Gumenitskiy RY, Chaban MY. Variation statistics course. Kyiv: High school, 1977. [Ukrainian].
- Schaffer SW, Azuma J, Mozaffar M. Role of antioxidant activity of taurine in diabetes. Can J Phys Pharm. 2009; 87: 91–99.
- Ribeiro RA, Bonfleur M L, Amaral AG. Taurine supplementation enhances nutrient-induced insulin secretion in pancreatic mice islets. Diabetes Metab Res Rev. 2009;25:370–9.