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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2015; 61(6): 76-85


T.V. Dovbynchuck1, L.V. Zakordonets, A.V. Putnikov, I. Vareniuk, О. Tiapko, N. Roslova, T. Sergiychuk, O. Lynchak, M. Dzerzhynsky, T. Beregova, G. Тоlstanova

    Taras Shevchenko National University of Kyiv, Kyiv;
  1. O.O. Bogomolets National Medical University, Kyiv


The aim of the present study was to investigate the effect of cephalosporin antibiotic ceftriaxone (50 mg/kg, i/m) and macrolide antibiotic azithromycin (15 mg/kg, per.os.) on net water transport across rat colonic epithelium. Study was done on male Wistar rats (180-250 g). Azithromycin or ceftriaxone was injected daily for 5 days. Net water transport was evaluated on the 6th day by isolated colonic loop perfusion technique in vivo on anaesthetized rats. Treatment with azithromycin increased 2,4-fold the absorption of water, while ceftriaxone caused decrease 1,9-fold water absorption. The antibiotics treatment within five days didn’t change the composition of the fecal and colonic parietal microbiota. Azithromycin-induced increase in water absorption was associated with upregulation of AQP 8 water channel expression (Р˂0,05) in colonic mucosa. Ceftriaxone treatment didn’t change protein level of AQP 8 but induced pro-inflammatory changes in colonic mucosa structure and mast cells degranulation. We showed for the first time the opposite effects of macrolide and cephalosporin antibiotics on net water transport across rat colonic epithelium.

Keywords: colon; ceftriaxone; azithromycin; diarrhea; AQP 8; mast cell.


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