FUNCTIONAL ACTIVITY OF EHRLICH CARCINOMA CELLS AFTER TREATMENT WITH HYBRID NANOCOMPLEXES CONTAINING ORTHOVANADATES OF RARE-EARTH ELEMENTS, CHOLESTEROL AND LUMINESCENT DYE
A.N. Goltsev1, N.N. Babenko1, Yu.A. Gaevskaya1, O.V. Chelombitko1, N.A. Bondarovich1, T.G. Dubrava1, M.V. Ostankov1, V.K. Klochkov2, N.S. Kavok2, Yu.V. Malyukin2
- Institute for Problems of Cryobiology and Cryomedicine of
the National Academy of Sciences of Ukraine, Kharkov;
- Institute for Scintillation Materials of the National
Academy of Sciences of Ukraine, Kharkov
DOI: https://doi.org/10.15407/fz61.06.060
Abstract
Tumor development is the consequence of expanding the population
of low differentiated cells with unlimited self-maintenance
potential, i.e. cancer stem cells (CSCs). Application of new
forms of nanocomposites capable of binding to CSCs and inducing
the tumor destruction is perspective direction for treating this
pathology. There have been developed the methods of obtaining
hybrid nanocomplexes containing rare-earth orthovanadates
GdYVO4:Eu3+, cholesterol and luminescent dye Dil. By immune
fluorescence method using monoclonal antibodies to CD44,
CD24, CD117 and Sca-1 markers there has been established the
change in the ratio of tumor progenitors of various differentiation
levels in a general pool of Ehrlich carcinoma (EC) after
treatment with hybrid nanocomplexes. Essential reduction in
the concentration of the most tumorogenic CD44high cells with
simultaneous rise in the number of CD117+-cells resulted in an
increased index of CD44high / CD117+ ratio. It has been demonstrated
that application of hybrid nanocomplexes suppressed
the tumor growth almost by 80%. The value of cooperative
interactions of the cells with different phenotype signs in tumor
sites has been proved. The index of CD44high / CD117+ ratio
can be used as one of diagnostic and prognostic parameters of
development and inactivation rate of tumor process when using
different types of anti-tumor therapy.
Keywords:
cancer stem cells; nanoparticles; Ehrlich carcinoma; orthovanadates.
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