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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2017 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2015; 61(6): 53-59


TISSUE BLOOD FLOW IN THE DIGESTIVE ORGANS OF RATS WITH ACUTE PANCREATITIS AFTER CORVITIN ADMINISTRATION

T.V. Vovkun1, P.I. Yanchuk1, L.Y. Shtanova1, A.S. Shalamaу2

  1. Kyiv Taras Shevchenko National University
  2. Closed Joint Stock Company “Borschagovsky ChemicalPharmaceutical Plant “, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz61.06.053

Abstract

We have investigated the action of quercetin (in a modified form – Corvitin, ВСРР, Ukrainе) on the rate of blood flow in the pancreas, liver and gastric mucosa of rats with acute pancreatitis (АР) caused by administration of L-arginine. The rate of blood flow was measured by hydrogen clearance method with electrochemical his generation using Polarographs Lr-9 (Czech Republic). During the first 10 days after modelling of АР in these organs it was observed a gradual decrease compared to the intact animals in the rate of blood flow by 42% (P<0,01) in the pancreas, by 61% (P<0,001) in the liver and by 64% (P<0,001) in the gastric mucosa, i.e., the most significant changes occurred in the gastric mucosa, the least – in the tissue of the pancreas. Compared with the control group of animals with modelling acute pancreatitis which during 20 days was administered only saline, application of Corvitin (5 mg/kg, 1 time per day from 11 to 20 days of experiment) in varying degrees promoted to the recovery of the rate of blood flow in all investigated organs: in the pancreas – fully, in the liver – almost entirely and in the gastric mucosa – only partially. Thus, based on obtained results Corvitin can be recommended for partial or complete correction of blood flow disturbances, which arise in the pancreas and other organs of the digestive system in AP. Corvitin can improve the functional state of these organs in the early stages of the disease and accelerate the full restoration of their functions.

Keywords: L-arginine; acute pancreatitis; pancreas; liver; gastric mucosa; Corvitin; rate of blood flow.

References

  1. Sah RP, Garg P, Saluja AK. Pathogenic mechanisms of acute pancreatitis. Curr Opin Gastroenterol. 2012;28(5):507-15. https://doi.org/10.1097/MOG.0b013e3283567f52 PMid:22885948 PMCid:PMC3682674
  2.  
  3. Zhou ZG, Chen YD. Influencing factors of pancreatic microcirculatory impairment in acute pancreatitis. World J Gastroenterol. 2002;8:406-412. https://doi.org/10.3748/wjg.v8.i3.406 PMCid:PMC4656410
  4.  
  5. McFadden DW. Organ failure and multiple organ system failure in pancreatitis. Pancreas. 1991;6:S37-S43.
  6.  
  7. Hegyi P, Rakonczay ZJ, Sári R, Góg C, Lonovics J, Takács T, Czakó L. L-arginine-induced experimental pancreatitis. World J Gastroenterol. 2004;10(14):2003-2009. https://doi.org/10.3748/wjg.v10.i14.2003 PMid:15237423 PMCid:PMC4572322
  8.  
  9. Sunamura M, Shibuya K, Yamauchi J, Matsuno S. Microcirculatory derangement and ischemia of the pancreas. Nihon Geka Gakkai Zasshi. 1999;100(5):342-6.
  10.  
  11. Dobosz M, Hac S, Mionskowska L, Dymecki D, Dobrowolski S, Wajda Z. Organ microcirculatory disturbances in experimental acute pancreatitis. A role of nitric oxide. Physiol Res. 2005;54(4):363-368.
  12.  
  13. Cuthbertson CM, Christophi C. Disturbances of the microcirculation in acute pancreatitis. Br J Surg. 2006;93(5):518-30. https://doi.org/10.1002/bjs.5316 PMid:16607683
  14.  
  15. Carvalho KM, Morais TC, de Melo TS, de Castro Brito GA, de Andrade GM, Rao VS. The natural flavonoid quercetin ameliorates cerulein-induced acute pancreatitis in mice. Biol Pharm Bull. 2010;33(9):1534-1539. https://doi.org/10.1248/bpb.33.1534 PMid:20823570
  16.  
  17. Moĭbenko OO, Sahach VF, Tkachenko MM, Korkushko OV, Bezrukov VV, Kulchytskyĭ OK, Stefanov OV, Solovĭov AI, Mala LT, Frol'kis VV. Mechanisms of nitric oxide activity in cardiovascular system as a basis of pathogenetic therapy of related diseases. Physiol Zh. 2004;50(1):11-30. [Ukrainian].
  18.  
  19. Pawlikowska-Pawlega B, Gruszecki WI, Misiak LE. The study of the quercetin action on human erythrocyte membranes. Biochem Pharmacol. 2003;66(4):605-12. https://doi.org/10.1016/S0006-2952(03)00344-7
  20.  
  21. Yu PX, Zhou QJ, Zhu WW, Wu YH, Wu LC, Lin X, Chen MH, Qiu BT. Effects of quercetin on LPS-induced disseminated intravascular coagulation (DIC) in rabbits. Thromb Res. 2013;131(6):e270-3.
  22.  
  23. Arikan S, Ersan I, Karaca T, Kara S, Gencer B, Karaboga I, Hasan Ali T. Quercetin protects the retina by reducing apoptosis due to ischemia-reperfusion injury in a rat model. Arq Bras Oftalmol. 2015;78(2):100-4. https://doi.org/10.5935/0004-2749.20150026 PMid:25945531
  24.  
  25. Loke WM, Hodgson JM, Proudfoot JM, McKinley AJ, Puddey IB, Croft KD. Pure dietary flavonoids quercetin and (-)- epicatechin augment nitric oxide products and reduce endothelin-1 acutely in healthy men. Am J Clin Nutr. 2008;88(4):1018-25.
  26.  
  27. Vovkun TV, Yanchuk PI, Shtanova LY, Veselskyu SP, Baranowskyy VA. Effect of Corvitin on Secretory Processes and Blood Flow in the Rat Gastric Mucosa. Int J Physiol and Pathophysiol. 2014;i4.70(4):335-43.
  28.  
  29. Vinogradova E, Pasichnichenko O, Vovkun T, Yanchuk P. Influence of corvitin on liver blood flow and serotonin on contractile activity of portal vein. Bull of Kyiv National Taras Shevchenko Univ. 2012;15:30-32. [Ukrainian].
  30.  
  31. Tashiro M, Schafer C, Yao H, Ernst SA, Williams JA. Arginine induced acute pancreatitis alters the actin cytoskeleton and increases heat shock protein expression in rat pancreatic acinar cells. Gut. 2001;49:241-250. https://doi.org/10.1136/gut.49.2.241 PMid:11454802 PMCid:PMC1728407
  32.  
  33. Yanchuk PI, Palatniy TP, Rusinchuk YI. Modified electrode for registration of local blood flow in the gastric mucosa by hydrogen clearance. Rus Physiol Journ. 2005;91(9):1108-1110. [Russian].
  34.  
  35. Foitzik T, Eibl G, Hotz HG, Faulhaber J, Kirchengast M. Endothelin receptor blockade in severe acute pancreatitis leads to systemic enhancement of microcirculation, stabilization of capillary permeability, and improved survival rates. Surgery. 2000;127:399-407. https://doi.org/10.1067/msy.2000.107104 PMid:10965310
  36.  
  37. Hegyi P, Takacs T, Jarmay K, Nagy I, Czako L, Lonovics J. Spontaneous and cholecystokinin-octapeptidepromoted regeneration of the pancreas following Larginine-induced pancreatitis in rat. Int J Pancreatol. 1997;22:193-200.
  38.  
  39. Hegyi P, Takacs T, Tiszlavicz L, Czako L, Lonovics J. Recovery of exocrine pancreas six months following pancreatitis induction with L-arginine in streptozotocindiabetic rats. J Physiol Paris. 2000;94:51-55. https://doi.org/10.1016/S0928-4257(99)00103-5
  40.  
  41. Hotz HG, Foitzik T, Rohweder J, Schulzke JD, Fromm M, Runkel NS, Buhr HJ: Intestinal microcirculation and gut permeability in acute pancreatitis: early changes and therapeutic implications. J Gastrointest Surg. 1998;2:518-525. https://doi.org/10.1016/S1091-255X(98)80051-6
  42.  
  43. Dang SCh, Zhang JX, Qu JG, Wang XQ. Ligustrazine alleviates gastric mucosal injury in a rat model of acute necrotizing pancreatitis. Hepatobiliary Pancreat Dis Int. 2007;6(2):213-218.
  44.  
  45. Dibirov AD, Petukhov VA, Donskoff MD. Changes of pankreatogepatoduodenal zone in experimental acute lipogenic pancreatitis. Bull Experim Biol and Medicine. 2000;130(8):232-236. [Russian].
  46.  
  47. Zіnenko DY, Beregovenko ІM. State of hepatic microcirculation and its role in the development of pathological changes in the liver when simulating acute pancreatitis in rats. Morphology. 2008;2(3):39-48. [Ukrainian].
  48.  
  49. Mozzhelin ME, Vengerovsky AI, Sukhodolov IV, Saratikov A. Liver damage in experimental acute pancreatitis. Bull of Experim Biol and Med. 2001;132(1):647-649. [Russian].
  50.  
  51. Panek J, Zasada J, Poźniczek M. Microcirculatory disturbance in the course of acute pancreatitis. Przegl Lek. 2007;64(6):435-7.
  52.  

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