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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2015; 61(5): 71-77


FUNCTIONING PROTEASES IN THE ESOPHAGUS MUCOSA AFTER CHEMICAL BURNS

T.V. Ishchuk, O.M Savchuk, Ya.B. Raetska, V.V. Vereschaka, L.I. Ostapchenko

    Educational and Scientific Centre «Institute of Biology», Taras Shevchenko National University of Kyiv
DOI: https://doi.org/10.15407/fz61.05.071

Abstract

The main result of esophagus burn is the formation of scars, that caused by excessive synthesis of collagen and changes the balance of metalloproteinases and their tissue inhibitors. It was studied the activity of proteolytic enzymes, participation of MMP (metalloproteinase) and their tissue inhibitors (TIMP) in alkali burns of the esophagus 1st and 2nd degrees. We have shown a significant increase of TIMP level in homogenate after alkali burns of the esophagus (an average of 31-56% depend on of burn degree). We observed a reduced activity of serine proteinase after 1st degree burns on 15th, 21st day 35 and 18% respectively, after burns 2nd degree on 15th, 21st day 54 and 50%. The decrease of activity MMP after 1st degree burns on 15th and 21st day 30, 19%, respectively, in conditions of chemical burns 2nd degree on 15th and 21st day 30, 37%. These data may indicate the development of scarring after burn simulation of 2nd degree. Further investigation of the MMP and TIMP in the process of wound healing can be useful in creating effective approaches to prevent formation of post scarring of the esophagus.

Keywords: caustic burns of the esophagus; matrix metalloproteinases; tissue inhibitor of metalloproteinases.

References

  1. Yong H, Qing-Shu C, Xiao-Fei L, Xiao-Ping W. Surgical management of esophageal strictures after caustic burns: A 30 years of experience. World J Gastroenterol. 2013; 10 (9): 2846-49.
  2.  
  3. Kalkan Y, Tumkaya L, Akdogan R, Yucel A, Tomak Y, Sehitoglu I, Pergel A. A novel model approach for esophageal burns in rats: A comparison of three methods. Toxicol Ind Health. 2013;1-7.
  4.  
  5. Fallahi S, Hosseini S, Fallahi S, Salimi M, Akbar Hesam A, Hamideh Hoseini S. Extent of Injury of Gastrointestinal tract due to accidental ingestion of chemicals among children at Bandar Abbass Children Hospital 2009–2011. Life Scі J. 2012; 9(4): 2054-58.
  6.  
  7. Savvy S. A modified classification of chemical esophageal burn and its consequencies. Clin Surgery. 2009; 3: 5-8 [Russian].
  8.  
  9. Boyko V, Kozin Y, Shevchenko A, Rajiv Kumar S, Novikov E, Klimenko V. Immunological disorders combined with post-burn strictures of the esophagus and stomach. Med Today and Tomorrow. 2009; 3–4: 92-5 [Russian].
  10.  
  11. Imaizumi R, Akasaka Y, Inomata N, Okada E, Ito K, Ishikawa Y, Maruyama Y. Promoted activation of matrix metalloproteinase (MMP)-2 in keloid fibroblasts and increased expression of MMP-2 in collagen bundle regions: implications for mechanisms of keloid progression. Histopathology. 2009; 54(6): 722-30. CrossRef PubMed
  12.  
  13. Gill S, Parks W. Metalloproteinases and Their Inhibitors: Regulators of Wound Healing. Int J Biochem Cell Biol. 2008; 40(6-7): 1334–47.
  14.  
  15. Bálint N, Lívia S, Szilárd R, Csaba L, Barbara R, János L, Lajos B, Csaba C. Dynamic changes of matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 after burn injury. J Critical Care 2015; 30 (1): 162-6. CrossRef PubMed
  16.  
  17. Raetska Y, Ischuk T, Dzhus O, Savchuk O, Ostapchenko L. Experimental modeling of 1st and 2nd degrees alkali esophageal burn in immature rats. Biological system. 2014; 6(1): 39 -44 (Ukrainian).
  18.  
  19. Savchuk O. The investigation of protein-protein interactions in hemostatic system using enzyme electroforesis method. Med Chemistry. 2010; 12(1): 60-7 [Ukrainian].
  20.  
  21. Hummel BC. Amodified spectrophotometric determination of chymotrypsin, trypsin, and thrombin. Can J Biochem and Physiol. 1959; 37: 1393-99. CrossRef  
  22. CrowtherJR, editor. The ELISAguidebook. Totowa, New Jersey: Humana Press. 2001.
  23.  
  24. McCarty S, Percival S. Proteases and Delayed Wound Healing. Adv Wound Care. 2013; 2(8): 438–47. CrossRef PubMed PubMedCentral
  25.  
  26. Mirastschijski U, Zhou Z, Rollman O, Tryggvason K, Ågren M. Wound Healing in Membrane-Type-1 Matrix Metalloproteinase-Deficient Mice. J Investigative Dermatology. 2004; 123: 600–602. CrossRef PubMed
  27.  
  28. Protasov MV, Smagina LV, Yudintseva NM, Galibin OV, Pinaev GP. Possibility of predicting rat wound epithelization by changes in matrix metalloproteinases activities in wound exudates. Cell and Tissue Biology. 2009; 3 (3): 249-253 [ Russian].
  29.  
  30. Dasu M, Spies M. Matrix metalloproteinases and their tissue inhibitors in severely burned children. Wound Rep Reg. 2003; 11:177-80. CrossRef  
  31. Dietmar U, Franziska U, Frank U, Andrzej P, Norbert P. Matrix metalloproteinases and tissue inhibitors of metalloproteinases in patients with different types of scars and keloids. J Plast Reconstr Aesthet Surg. 2010; 63: 1015-21. CrossRef PubMed
  32.  
  33. Frossing S, Rono B, Hald A, Romer J, Lund LR. Skin wound healing in MMP2-deficient and MMP2 / plasminogen double-deficient mice. Exp Dermatol. 2010; 19 (8): 234-40. CrossRef PubMed
  34.  
  35. Zheng L, Amano K, Iohara K, Ito M, Imabayashi K., Into T, Matsushita K. Matrix metalloproteinase-3 accelerates wound healing following dental pulp injury. Am J Pathol. 2009; 175: 1905-14. CrossRef PubMed PubMedCentral
  36.  
  37. Nwomen B, Hui-Xiu L, Diegelmann R, Coner K. Dynamics of the matrix metalloproteinases MMP-1 and MMP-8 in acute open human dermal wounds. Wound Rep Reg. 1998; 6: 127-36. CrossRef  
  38. Li Q, Guo S, Liu D, Feng S, Wei Q. Correlation of the expressions of MMPs-9, TIMP-1 and TIMP-2 with cesarean section scar. Nan Fang Yi Ke Da Xue Xue Bao. 2012; 32 (9): 1336-40.
  39.  

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