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Fiziologichnyi Zhurnal - <a id="lj1_ret_bk_to_jl0_href_id" href="?list0=17">Fiziol. Zh. 2015;</a> <a id="lj1_ret_bk_to_jl0_href_id" href="?list1=90">61(5):</a> 65-70
Українська Русский English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2015; 61(5): 65-70


DYNAMICS OF GLUTAMINE SYNTHASE ACTIVITY IN RAT BRAIN IN PRENATAL HYPOXIA MODEL

V.R. Khairova, M.I. Safarov

    Institute of Physiology, Azerbaijan National Academy of Science, Baku, Azerbaijan
DOI: https://doi.org/10.15407/fz61.05.065

Abstract

Prenatal ontogenesis is a period of high sensitivity to stressful impact, so any stressor can lead to changes of physiological, biochemical indicators, behavioral and cognitive functions. The most common and clinically significant stress factor, which the embryo may be exposed during embryonic development, is hypoxia. In this case pathological changes in the central nervous system depend on the duration and severity of hypoxic exposure, individual tolerance and the stage of prenatal development, at each of which in the brain take place the basic histogenetic processes. By activating energetically disadvantageous anaerobic glycolysis hypoxia leads to excess of glutamate emission and cell apoptosis. Glutamine synthase is a basic enzyme that regulates metabolism of glutamate, catalyzing conversion of glutamate to glutamine with ammonia detoxification. The aim of the presented work was to reveal changes in the activity of one of the key enzyme of glutamate metabolism– glutamine synthetase - in the brain of offspring of white rats undergone to hypoxia at different stages of prenatal ontogenesis. Hypoxia was created by placing female rats at stages of the pregnancy, corresponding to progestation period of organogenesis and fetal period of prenatal development, in the hypobaric chamber with exposure to 5% oxygen and 95% nitrogen gas mixture during 30 minutes per day. The offspring obtained from females of control and experimental groups were used for biochemical determinations in the age of 1 and 3month. It has been established that hypoxia exposed to pregnant females during embryonic organogenesis causes significant changes in enzyme activity, particularly pronounced in the cerebral cortex and cerebellum, as compared with progestational and fetal hypoxia. Enzyme activity decreased in a greater degree in one-month-old rats undergone to prenatal hypoxia, than three- month-old animals. Thus, stress during intensive processes of proliferation and migration of cells of the forming brain violates glutamate metabolism of the brain.

Keywords: glutamine synthetase; prenatal hypoxia; brain; ontogenesis.

References

  1. Ignat'eva R.K. Perinatal'nye problemy v Rossii [Perinatal problems in Russia]. Moscow, 2006. 40 p [Russian].
  2.  
  3. Graf A. V., Maklakova A.S., Maslova M.V. Vliyanie prenatal'noi gipoksii, provedennoi na stadii organogeneza, na povedenie belykh krys v postnatal'nom ontogeneze. The impact of prenatal hypoxia conducted at the stage of organogenesis, on behavior of white rats in postnatal ontogenesis. Izv. Ros. Akad. Nauk = Math. Ros. Acad. Sci 2006; 4: 476-481. [Russian].
  4.  
  5. Delcour M., Russier M., Amin M. et al. Impact of prenatal ischemia on behavior, cognitive abilities and neuroanatomy in adult rats with white matter damage. Behav. Brain Res. 2012; 232(1): 233- 244.
  6.  
  7. Basovich S.N. Gipoksiya v geneze i terapii mental'nykh otklonenii: obzor [Hypoxia in the genesis and treatment of mental abnormalities: a review]. Vestn. Mezhd. Akad. Nauk, Rus. sektsiya = Bull. of the Inter. Acad. Sci., Russ. section 2011; ;1 [Russian].
  8.  
  9. Zhuravin I.A, N. L. Tumanova N.L., Vasil'ev D.S. Izmenenie adaptivnykh mekhanizmov mozga v ontogeneze krys, perenesshikh prenatal'nuyu gipoksiyu [Changing the adaptive mechanisms of the brain during ontogenesis in rats undergoing prenatal hypoxia]. Dokl. Akad. Nauk = Dokl. Acad. Sci 2009; 425 (1):123-125 [Russian].
  10.  
  11. Slobodin V.B., Tomilova I.K., Kadykova E.L., Bogatov D.N.. Svobodno-radikal'nye protsessy i osobennosti metabolizma glutamata v golovnom mozge novorozhdennykh krys, razvivavshikhsya pri nedostatochnosti matochnoplatsentarnogo krovoobrashcheniya [Free-radical processes and features of glutamate metabolism in the brains of newborn rats that developed at insufficiency of uteroplacental circulation]. Pat. fiziol. i eksp. terapiya = Pathol. physiol. and exp. terapiya 2007; (2): 17-19 [Russian].
  12.  
  13. Fadeeva N.I., Khovalyg N.M., Remneva O.V. Oksidantnoantioksidantnyi status nedonoshennykh novorozhdennykh s perinatal'nym porazheniem tsentral'noi nervnoi sistemy [Oxidant-antioxidant status in preterm infants with perinatal lesions of the central nervous system]. Ross. Vestnik perinat. i pediatrii = Russ. Bull. perinat. and pediatrics 2010; 55(3):26-30 [Russian].
  14.  
  15. Boldyrev A.A. Vzaimodeistviya mezhdu glutamatnymi retseptorami [Interactions between glutamate receptors]. Byul. eksperim. boil. i meditsiny = Bull. exper. biol. and medicine 2000; 130( 9): 244 – 251 [Russian].
  16.  
  17. Bol'shakov A.P. Glutamatnaya neirotoksichnost': narusheniya ionnogo gomeostaza, disfunktsiya mitokhondrii, izmenenie aktivnosti kletochnykh system [Glutamate neurotoxicity: violation of ion homeostasis, mitochondrial dysfunction, changes in the activity of cellular Dinamika glutaminsintetaznoi aktivnosti v golovnom mozgu kris systems]. Neirokhimiya = Neurochemistry 2008; 25( 3): 157-169 [Russian].
  18.  
  19. Samoilov M.O., Mokrushin A.A., Semenov D.G. Vovlechenie glutamatnykh retseptorov (NMDA-tipa) v reaktsii neironov mozga na anoksiyu razlichnoi dlitel'nosti [Involving glutamate receptors (NMDAtype) in response to the neurons in the brain anoxia of various duration]. Vestn. RAMN = Vestn. RAMS 2000; (9): 34-39 [Russian].
  20.  
  21. Silakov A.I., Trubin G.P., Yavilyakova A. Mikrometod opredeleniya ammiaka i glutamina v tkanyakh GMKh ekstraktakh [Micromethods determination of ammonia and glutamine in the tissue extracts GMH]. Vopr. med. khimii = Probl. Med. chemistry 1962; 8(5): 538-544 [Russian].
  22.  
  23. Gadirova L.B., Abbasova M.T., Khairova V.R. Vliyanie gipoksii, perenesennoi v post- i prenatal'nyi periody ontogeneza, na aktivnost' nekotorykh fermentov obmena glutamata v mozge krys [The impact of hypoxia in the pre- and postnatal periods of development on the activity of certain enzymes of glutamate metabolism in the rat brain]. «Izvestiya» NAN Azerbaidzhana. Baku. Elm = Proceedings of National Academy of Sciences of Azerbaijan. Baku. Elm 2010; (1-2):100-104 [Russian].
  24.  
  25. Mikheeva I.G., Ryukert E.N., Brusov O.S. Soderzhanie serotonina v syvorotke krovi novorozhdennykh detei s gipoksicheski-ishemicheskim porazheniem TsNS [The content of serotonin in the blood serum of neonates with hypoxic-ischemic CNS]. Pediatriya = Pediatrics 2008; (1): 40–44 [Russian].
  26.  

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