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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2015; 61(5): 57-64


Expression of aryl hydrocarbon receptor and ATG16L1 protein in experimental oxazolone-induced colitis in rats

A.S. Zherebiatiev, A.M. Kamyshny

    Zaporozhye State Medical University, Zaporozhye, Ukraine
DOI: https://doi.org/10.15407/fz61.05.057

Abstract

We studied the expression of AhR and ATG16L1 protein in experimental oxazolone-induced colitis in rats and anti-inflammatory action of recombinant antagonist of IL-1 receptors (ARIL-1) and simvastatin. The immunopositive cells were determined using an indirect immunofluorescence technique with using a monoclonal rat antibody. It has been established that development of colitis was accompanied by an increase of total number of ATG16L1+-lymphocytes (by 30%, P <0.05) in lymphoid structures of the colon. However, the amount of AhR+-lymphocytes has not changed. At the same time has increased the concentration of ATG16L1 protein (by 4-11%, P <0.05) in immunopositive cells. Administration of simvastatin and ARIL-1 during the development of experimental pathology was accompanied by decrease of total number of AhR+ (by 24-38%, P <0.05) and ATG16L1+-lymphocytes (by 43% - 2 fold, P <0.05) in the colon.

Keywords: colitis; recombinant antagonist of receptors of interleukin-1 (ARIL-1); simvastatin; aryl hydrocarbon receptor; autophagy related 16-like 1 protein.

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