THE STUDY OF THE RELATIONSHIP BETWEEN THE ACTIVITY OF EPIPHYSIS AND GONADS IN MALE RATS IN DIFFERENT SEASONS
V. Hnatiuk, N. Kononenko
National University of Pharmacy, Kharkiv, Ukraine
In male rats of different ages, we studied the functional activity
of the pineal gland and the gonads during different seasons
by determining the levels of melatonin and testosterone in the
blood serum. It was found that the highest levels of melatonin
observed in male rats in summer and winter, and the lowest – in
autumn. The lowest level of melatonin has been established
in the group of rats aged 9 months – 127.28+5.11 pmol/l that
was 22% lower than the level of melatonin in 3 months old
rats (P≤0,05), and 20% below the level of melatonin in rats of
15 months old (P≤0,05). The highest level determined in rats
aged 3 months (corresponds to the age of man –14 years old),
the lowest – in rats 20 months (corresponds to the age 55-56
years old). At the same time, the low level of melatonin was
defined in rats aged 9 months in the autumn (corresponds
to the age of man 29-30 years). In the study of testosterone
levels in different seasons it was found that the highest level
in all age groups presents in autumn, the lowest – in winter.
When comparing the levels of testosterone in different age
groups, it was determined that the fall of the highest level
of testosterone present in the blood of males age 9 months
and 15 months, which corresponds to the age of 29-30 and
43-44 of human years. Significant difference between the
levels of testosterone in the groups that correspond to the
reproductive age – rats of age 3, 9 and 15 months, are present
only in the autumn; spring and summer – significant
differences between the levels of testosterone absent. The
degree of correlation differed in rats of different ages in
different seasons: the highest correlation between the levels
of melatonin and testosterone was determined in rats aged 9
months in autumn. The correlation coefficients in rats aged
20 months old have been weak - from 0.05 in the spring to
0.17 in autumn that shows the changes in neurohormonal
regulation which occur with age.
epiphysis; gonads; melatonin; testosterone; seasons; sex; age.
- Korkushko OV, Shatilo VB. Functional status pineal gland of the brain with aging: ways of correction desynchronosis. Bukovin Med Visnik. 2006;4(10):8–10. [Ukrainian].
- Komarov FI, Rapoport SI, Malinovskaia NK, Anisimov VN. Melatonin in health and disease. Moscow; 2004.[Russian].
- Anisimov VN. Chronometer of life. Priroda. 2007;7:3–10. [Russian].
- Yanko RV. Morphofunctional state of the thyroid gland after exposure to melatonin. Fiziol Zh. 2015;5(61):47–51. [Ukrainian].
- Kaladze NN, Soboleva EM, Skoromnaia NN. Results and prospects of physiological, pathogenetic and pharmacological effects of melatonin. Zdorov Reb. 2010; 2(32):156–66. [Russian].
- Minukhin AS. The role of androgens in the provision of sexual function in men. Probl Endocr Path. 2010; 1:99–106. [Russian].
- Minukhin AS, Bondarenko VA. The role of hormonal factors in the regulation and maintenance of sexual function of men. Probl Endocr Path. 2011; 1:76–82. [Russian].
- Kostenko E, Manevich TM, Razumov NA. Desynchronosis as One of the Most Important Factors of Cerebrovascular Disease. Lecheb Delo. 2013; 2:104–116. [Russian].
- Osnovni prichini visokogo rivnya smertnosti v Ukrayini. News Med and Pharm. 2010; 22 (350):URL: http:// www.mif-ua.com/archive/article/15170. (Accessed 01.04.2016). [Russian].
- Brambilla DJ, Matsumoto AM, Araujo AB, McKinlay JB. The effect of diurnal variation on clinical measurement of serum testosterone and other sex hormone levels in men. J Clin Endocrinol Metab. 2009; 94(3):907–13.
- Korkushko OV, Khavinson VKh, Shatilo VB, Pisaruk AV, Labunets IF. Diurnal rhythms of changes in the function of cardiovascular system and its autonomic regulation in apparently healthy elderly subjects with special reference to the state of melatonin-producing function of the epiphysis. J Acad Med Sci Ukr. 2005; 1 (11):136–47.
- Mukaka MM. Statistics corner: A guide to appropriate use of Correlation coefficient in medical research. Malaw Med J Sept. 2012; 24(3): 69–71.
- Kochetov AG, Liang OV, Masenko VP, Zhirov IV, Nakonechnikov SV, Tereshchenko SN. Statistical methods of medical data. Moscow, 2012. [Russian]
- Bondarenko LA. Comparative estimation of season influence on the night melatonin concentration peak formation in young and old rats. Physiol J. 1992; 2(38):111–14. [Russian].
- Bartsch H, Bartsch C, Mecke D, Lippert TH. Seasonality of pineal melatonin production in the rat: possible synchronization by the geomagnetic field. Chronobiol Int. 1994; 11(1):21–6.
- Rapoport SI, Malinovskaya NK. Epiphysis – the targetorgan of biotropic action of natural magnetic waves. Bukov Med Herald. 2006; 4(10):13–15. [Russian].
- Bondarenko LA. The role of interactions between internal and external environmental factors in regulation of pineal gland functional activity. Manuscript. Kyiv, 2003. P.10. [Russian].
- Belenichev IF, Gubskiy YuI, Llevitsky EL, Kovalenko SI, Marchenko AN. Regulation of antioxidant homeostasis and system detoxication of organizm by hormone melatonin. Role of melatonin dependent receptors in realization of this function. Mod Probl Toxicol. 2003; 2:2–16. [Russian].
- Johnsson A. Light, circadian and circannual rhythms. Solar Radiat Human Health. Oslo, 2008. P. 57–75.
- Agadzhanian NA, Radish IV, Khisamutdinov AF. The lipid and hormone metabolism in healthy men in different seasons. Kazan Med J. 2009; 6 (90):776–79. [Russian].
- Bondarenko LA. Some hormonal mechanisms of accelerated aging at gipopinealizme. Kharkiv, 2015. [Russian].
- Iarygina VN, Melenteva AS. Manual Gerontology and Geriatrics. Moscow, 2010. [Russian].
- Belichev IF, Gubskii YI, Levickii EL, Kovalenko SI, Marchenko AN. Regulation of antioxidant homeostasis and detoxification system of the body of the hormone melatonin. The role of melatonin-dependent receptors in this function. Such Probl Toksik. 2003; 2:2–16. [Russian].