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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2016; 62(6): 43-49


Polymorphism Pro12→Ala of the peroxisome proliferator-activated receptors gamma gene effect on lipid peroxidation and antioxidant defense in patients with type 2 diabetes

V.Y. Mokrii, S.V. Ziablytsev

    O.O. Bogomolets National Medical University, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz62.06.043

Abstract

In this research, we discover effect of polymorphism rs1801282 of the PPARG gene on the formation oxidative stress in patients with type 2 diabetes, depending on the duration of the disease: till 5 years, 5-10 years and more than 10 years. Activity of lipid peroxidation was assess in terms of diene conjugates (DC) and malonic dealdehid (MDA) and antioxidant system status - the activity of superoxide dismutase (SOD), catalase and the level of α-tocopherol (α-TF). Molecular genetic studies conducted by the method of polymerase chain reaction (PCR) in real time. During disease 5-10 years, found increase levels of DC and MDA in patients with polymorphism Pro12→Ala for 34.9 and 34.7%, in compared with Pro12→Pro (P=0.01). Availability Pro12→Pro stipulated reduction of catalase activity during disease 5-10 years at 75% (P=0.001), and for those, who are ill for more than 10 years at 2,04 times (P=0.01), which is not different from the reference level (F=1.19; P=0.600), but in the case Pro12→Ala, this figure was in 2 times higher. The main conclusion is that the type Pro12→Ala of polymorphism rs1801282 of the PPARG gene causes the development of oxidative stress in patients with type 2 diabetes with 5-10 years durations, and Pro12→Pro - deficiency the enzyme catalase level of antioxidant system in patients with durations of disease more than 5 years.

Keywords: polymorphism Pro12→Ala of the PPARG gene; rs1801282; type 2 diabetes; oxidative stress

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© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2019.