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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2016; 62(6): 43-49

Polymorphism Pro12→Ala of the peroxisome proliferator-activated receptors gamma gene effect on lipid peroxidation and antioxidant defense in patients with type 2 diabetes

V.Y. Mokrii, S.V. Ziablytsev

    O.O. Bogomolets National Medical University, Kyiv, Ukraine


In this research, we discover effect of polymorphism rs1801282 of the PPARG gene on the formation oxidative stress in patients with type 2 diabetes, depending on the duration of the disease: till 5 years, 5-10 years and more than 10 years. Activity of lipid peroxidation was assess in terms of diene conjugates (DC) and malonic dealdehid (MDA) and antioxidant system status - the activity of superoxide dismutase (SOD), catalase and the level of α-tocopherol (α-TF). Molecular genetic studies conducted by the method of polymerase chain reaction (PCR) in real time. During disease 5-10 years, found increase levels of DC and MDA in patients with polymorphism Pro12→Ala for 34.9 and 34.7%, in compared with Pro12→Pro (P=0.01). Availability Pro12→Pro stipulated reduction of catalase activity during disease 5-10 years at 75% (P=0.001), and for those, who are ill for more than 10 years at 2,04 times (P=0.01), which is not different from the reference level (F=1.19; P=0.600), but in the case Pro12→Ala, this figure was in 2 times higher. The main conclusion is that the type Pro12→Ala of polymorphism rs1801282 of the PPARG gene causes the development of oxidative stress in patients with type 2 diabetes with 5-10 years durations, and Pro12→Pro - deficiency the enzyme catalase level of antioxidant system in patients with durations of disease more than 5 years.

Keywords: polymorphism Pro12→Ala of the PPARG gene; rs1801282; type 2 diabetes; oxidative stress


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