Українська English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2004; 50(2): 43-48

SOME MECHANISMS OF ENDOTHELIAL DYSFUNCTION IN AGING

O. V. Korkushko1, V. F. Sagach2, V. Yu. Lishnevskaya1

  1. Institute of Gerontology, AMS of Ukraine, Kiev
  2. A.A. Bogomoletz Institute of Physiology, National Academy of Sciences of Ukraine, Kiev



Abstract

Taking into consideration age-related changes in endothelium, the ability of endothelium to produce vasoactive substances has been studied in healthy subjects of various age. Under experimental conditions, the activity of constitutive and inducible NO-synthase has been studied as well. The clinical part of the study involved investigation of 38 healthy subjects aged 60-79. The study has been designed to investigate vasomotor function of the endothelium, the levels of endothelin-1, NO2, prostacyclin, thromboxan, the level of adhesive molecules and inhibitor of tissue plasminogen activator. The levels of endothelial and inducible NO-synthases in tissues of the myocardium and the aorta has been investigated in healthy rats of various age. The data obtained show a decrease in the production of relaxation factors: NO and prostacyclin with aging. An important mechanism of deterioration of NO-synthesizing function of the endothelium is a decrease in endothelial NOS activity. The synthesis of endothelial vasoconstricting agents increased with ageing. This in combination with decreased production of vasodilating substances results in deterioration of defence properties of the endothelium. The increase in titer of adhesive molecules and inhibitors of tissue plasminogen activator testifes to agedependent deterioration of anti-inflammatory and antithrombotic activities of the endothelium.

Full text: (PDF, in Ukrainian)

References

  1. Коркушко О. В., Лишневская В. Ю., Саркисов К. Г., Дужак Г. В. Функциональное состояние эндотелия сосудов у практически здоровых людей пожилого возраста. В кн.: Биологические механизмы старения: IV Междунар. симпоз.: Тез. 2427 мая 2000. Харьков. С. 83.
  2. Малая Л.Т., Корж А.Н., Балковая Л.Б. Эндотелиальная дисфункция при патологии сердечно-сосудистой системы. Х: Торсинг. 2000. 432 с.
  3. Chin S.Y., Pandey K.N., Shi S.J. et al. Increased activity and expression of Ca2+-dependent NOS in renal cortex of ANG II-infused hypertensive rats // Amer. J. Physiol. 1999. 277, 5. P.797-804.
  4. Forstermann U. Nitric oxide in the pathogenesis of vascular disease. J. Рathol. 2000. 90. P.244-254.
  5. Gewaltig M., Kojda G. Vasoprotection by nitric oxide: mechanisms and therapeutic potential // Circulat. Pes. 2002. 55. P. 250-261.
  6. Green L.L., Wagner D.A., Glogowski J. et al. Analysis of nitrate, nitrite and [+5N] nitrate in biological fluids // Anal. Biochem. 1982, № 1. P.131-138.
  7. Grundy S. Age as a risk factor: you are as old as tour arteries // Amer. J. Сardiol. 1999. 83, № 5. P.1455-1457.
  8. Halcox J. Endothelial dysfunction and prognosis //Circulation. 2002. 106. P.659-669.
  9. Halcox J.P.J., Schcnkc W.H., Zalos G. еt al. Prognostic value of coronary vascular endothelial function. 2002. 106. P.653-658.
  10. Hambrecht R., Wolf A., Gielen S. et al. Effect of exercise on coronary endothelial function in patients with coronary artery disease //N. Engl. J. Med. 2000. 342. P.454-460.
  11. L.J., Cirino G., Casini A., Napoli C. Nitric oxide as a signaling molecule in the vascular system: an overview //J. Cardiovasc. Pharmacol. 1999. 34. P.879-886.
  12. Jem S., Wall U., Bergbrant A. et al. Endothelium-dependent vasodilation and tissue-type plasminogen activator release in borderline hypertension //Arterioscler. Thromb. Vase Biol. 1997. 17. P.3376-3383.
  13. Kannel W. Coronary heart disease risk factors in the elderly // Amer.J. Geriatric. Сardiolog. 2002. 9, №2. P. 101-108.
  14. Kojda G., Cheng Y.C., Burchfield J., Harrison D.G. Dysfunctional regulation of endothelial nitric oxide synthase (eNOS) expression in response to exercise in mice lacking one eNOS gene //Circulation. 2001. 103. P.2839-2844.
  15. Korkushko O., Stupina A., Sarkisov K., Lishnevskaya V. Morpho-functional state of vessels endothelium in healthy elderly //Biogerontology. 2002. 3, supl.1. P. 66-67.
  16. Li H., Forstermann U. Nitric oxide in the pathogenesis of vascular disease //J. Pathol. 2000. 190(3). P.244-254.
  17. Lucas K.A., Pitari G.M., Kazerounian S. et al. Guanylyl cyclases and signaling by cyclic GMP //Pharmacol. Rev. 2000. 52. P.375-414.
  18. Selter M., Knowles R.G., Moncada S. Widespread tissue distribution, species and changes in activity of Ca2+-dependent and 2+-independent nitric oxide syntases // FEBS Lett. 1991. 291 P. 145-149.
  19. Vodovotz Y., Know N.S., Popischil M. et al. Inactivation of nitric oxide synthase after prolonged incubation of mouse macrophages with IFN-gamma and bacterial lipopolysaccharide // J.Immunol. 1994. 152, №8. P. 4110-4118.

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2024.